2022
DOI: 10.1002/iub.2685
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Adaptation of cardiomyogenesis to the generation and maturation of cardiomyocytes from human pluripotent stem cells

Abstract: The advent of methods for efficient generation and cardiac differentiation of pluripotent stem cells opened new avenues for disease modelling, drug testing, and cell therapies of the heart. However, cardiomyocytes (CM) obtained from such cells demonstrate an immature, foetal‐like phenotype that involves spontaneous contractions, irregular morphology, expression of embryonic isoforms of sarcomere components, and low level of ion channels. These and other features may affect cellular response to putative therape… Show more

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Cited by 3 publications
(4 citation statements)
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“…Furthermore, when these are short and highly functionalized, high biodegradability can be achieved; 3) In-mass modification of scaffolds with bioactive molecules for their controllable release. The most promising ones include: albuminmodified fatty acids (which could force a switch in the CM metabolic pathway), triiodothyronine (inducing a switch from fetal N2BA to an adult N2B titin isoform) and dexamethasone (both of which affect the CM electrophysiology, and IGF-1 (to induce the hyperplastic growth)) (Martyniak et al, 2023). Preferably, these should be released in a switchable manner, so as to mimic their natural occurrence within the developing and maturing heart; 4) A co-culture with cardiac fibroblasts which could aid in scaffold remodeling and secrete specific signaling molecules that further drive the CM maturation; 5) Introducing exogenous electrical and mechanical stimulation which could "train" the cardiac tissue, thus forcing CM maturation; and 6) Finally, in our opinion, CM culture should be dynamic-the mechanical and electrical properties of the growing tissue should be controllable.…”
Section: Discussionmentioning
confidence: 99%
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“…Furthermore, when these are short and highly functionalized, high biodegradability can be achieved; 3) In-mass modification of scaffolds with bioactive molecules for their controllable release. The most promising ones include: albuminmodified fatty acids (which could force a switch in the CM metabolic pathway), triiodothyronine (inducing a switch from fetal N2BA to an adult N2B titin isoform) and dexamethasone (both of which affect the CM electrophysiology, and IGF-1 (to induce the hyperplastic growth)) (Martyniak et al, 2023). Preferably, these should be released in a switchable manner, so as to mimic their natural occurrence within the developing and maturing heart; 4) A co-culture with cardiac fibroblasts which could aid in scaffold remodeling and secrete specific signaling molecules that further drive the CM maturation; 5) Introducing exogenous electrical and mechanical stimulation which could "train" the cardiac tissue, thus forcing CM maturation; and 6) Finally, in our opinion, CM culture should be dynamic-the mechanical and electrical properties of the growing tissue should be controllable.…”
Section: Discussionmentioning
confidence: 99%
“…Important advantages of using iPSCs over other cell sources are their unlimited source and ability to obtain patient-specific cardiomyocytes. The latter becomes favorable when considering tissue engineering strategies with a reduced rejection risk, as well as designing strategies for the in vitro evaluation of various genetic diseases (Martyniak et al, 2023). Pluripotent cells can be subjected to initial differentiation, which already has been proven to yield cardiomyocytes in vitro.…”
Section: Cell Sourcesmentioning
confidence: 99%
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