Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Guillen-Ahlers, Hector; Rao, Prahlad K.; Perumalla, Danu S.; Montoya, María; Jadhav, Avinash Y.L.; Shortreed, Michael R.; Smith, Lloyd M.; Olivier, Michael

doi:10.3791/57140-v

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study utilized label-free ion chromatogram peak area measurements. Considerations for adaptation of HyCCAPP in mammalian cells vs. the yeast system it was originally optimized in have been reviewed elsewhere [112]. A sequential probe release strategy enabled a multiplexed HyCCAPP approach that allowed for the capture of multiple single-copy loci in a single experiment, expanding the experimental capabilities of the system [113].…”

Section: Hybridization Capture Of Chromatin-associated Proteins For P...mentioning

confidence: 99%

Reverse-ChIP Techniques for Identifying Locus-Specific Proteomes: A Key Tool in Unlocking the Cancer Regulome

MacKenzie

Cisneros

Maynard

et al. 2023

Cells

View full text Add to dashboard Cite

A phenotypic hallmark of cancer is aberrant transcriptional regulation. Transcriptional regulation is controlled by a complicated array of molecular factors, including the presence of transcription factors, the deposition of histone post-translational modifications, and long‑range DNA interactions. Determining the molecular identity and function of these various factors is necessary to understand specific aspects of cancer biology and reveal potential therapeutic targets. Regulation of the genome by specific factors is typically studied using chromatin immunoprecipitation followed by sequencing (ChIP‑Seq) that identifies genome-wide binding interactions through the use of factor-specific antibodies. A long-standing goal in many laboratories has been the development of a ‘reverse-ChIP’ approach to identify unknown binding partners at loci of interest. A variety of strategies have been employed to enable the selective biochemical purification of sequence-defined chromatin regions, including single-copy loci, and the subsequent analytical detection of associated proteins. This review covers mass spectrometry techniques that enable quantitative proteomics before providing a survey of approaches toward the development of strategies for the purification of sequence-specific chromatin as a ‘reverse-ChIP’ technique. A fully realized reverse-ChIP technique holds great potential for identifying cancer-specific targets and the development of personalized therapeutic regimens.

show abstract

Section: Hybridization Capture Of Chromatin-associated Proteins For P...mentioning

confidence: 99%

Reverse-ChIP Techniques for Identifying Locus-Specific Proteomes: A Key Tool in Unlocking the Cancer Regulome

MacKenzie

Cisneros

Maynard

et al. 2023

Cells

View full text Add to dashboard Cite

show abstract

Adaptation of Hybridization Capture of Chromatin-associated Proteins for Proteomics to Mammalian Cells

Cited by 1 publication

References 0 publications

Reverse-ChIP Techniques for Identifying Locus-Specific Proteomes: A Key Tool in Unlocking the Cancer Regulome

Reverse-ChIP Techniques for Identifying Locus-Specific Proteomes: A Key Tool in Unlocking the Cancer Regulome

Contact Info

Product

Resources

About