Adaptation of <i>Escherichia coli</i> ATCC 8739 to 11% NaCl

How, Jian Ann; Lim, Joshua Zr; Goh, Desmond Jw; Ng, Wei Chuan; Oon, Jack S. H.; Lee, Kun Cheng; Lee, Chin How; Ling, Maurice Ht

doi:10.7167/2013/219095

Cited by 13 publications

(12 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Potassium chloride (1.5 %) decreased the growth of another strain of E. coli (Abdulkarim et al, 2009). In contrast to that, E. coli ATCC 8739 was adapted to 11 mass % of salt (How et al, 2013) and E. coli K-l2 MC 4100 to sea water (Troussellier et al, 1998). Fluoride negatively influenced the growth of E. coli (Baker et al, 2012), but the growth of E. coli BL21-pGFPuv was increased by low concentrations (0.1-10.0 mmol L −1 ) of sodium fluoride (Ma et al, 2014).…”

Section: Introductionmentioning

confidence: 87%

Whole-cell optical biosensor for mercury – operational conditions in saline water

2015

View full text Add to dashboard Cite

The present study demonstrates the influences of chlorides, fluorides and bromides of potassium and sodium on the growth and Hg 2+ -induced bioluminescence of bioreporter Escherichia coli ARL1. In a Luria-Bertani medium (LB), cell growth was inhibited by concentrations of sodium and potassium fluorides above 0.2 mol L −1 . The addition of NaCl increased cell tolerance to the toxic effects of fluorides and bromides. Lag periods of 10 h and more were observed for cultivations in LB without NaCl and with halides (NaCl, KCl, NaBr, KBr, NaF and KF) at concentrations lower than 0.06 mol L −1 . In a phosphate buffer (PB), the bioluminescence of E. coli ARL1, induced with HgCl2, was increased by the addition of NaCl, KCl, NaBr, KBr, NaF and KF (concentration of 0-0.25 mol L −1 ). In a saline phosphate buffer (PBS), the maxima of induced bioluminescence declined to 50 %, in the case of NaF (0.12 mol L −1 ), and to zero for KF. An addition of tryptone to the induction medium increased induced light emission ten-fold. Concentrated artificial sea water (ASW) (70-100 % ASW) inhibited bioluminescence induction. The new detection assay with E. coli ARL1 made possible the detection of 0.57 µg L −1 of HgCl2 in double-diluted artificial sea water (25 % ASW).

show abstract

Section: Introductionmentioning

confidence: 87%

Whole-cell optical biosensor for mercury – operational conditions in saline water

2015

View full text Add to dashboard Cite

show abstract

“…According to data from the Ministry of Health, milk and its derivatives are among the foods involved in food outbreaks in the country from 2000 to 2017 (Brasil, 2018). Some authors indicate that E. coli and S. aureus are among the main etiological agents involved in these outbreaks (Brasil, 2018) and both microrganisms have the capacity of to develop in environments with high salinity (How et al, 2013) Dther microorganisms such as yeast Candida albicans can also contaminate food by manipulation (Medeiros et al, 2012); this microorganism is an opportunistic pathogen which can proliferate when there is an imbalance of the microbiota in the environment also can develop in environments with low water activity (Tortora et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Influence of the salt concentration on action mechanisms of natamycin against microorganisms of importance in food manufacture

et al. 2020

View full text Add to dashboard Cite

The objective of this study was to evaluate the influence of salt concentration on action of natamycin on important microorganisms in food manufacturing as this preservative has been used in immersion baths in several dairy products in the country. Strains of Candida albicans, Escherichia coli and Staphylococcus aureus were inoculated at different saline and peptone water concentrations and received natamycin treatments. These solutions were maintained at 12 °C and the behavior of the microorganisms evaluated at 0, 24 and 48 hours (T0, T1 and T2). Each microorganism was assessed in isolation as well as the association of C. albicans and E. coli. Under the conditions proposed by the research, it was possible to conclude that 0.025% natamycin has no efficacy on C. albicans inoculated at saline concentrations below 5%. The results obtained in E. coli counts suggest that natamycin may interfere with its development even at concentrations that may be considered low (0.1%) and at salinity conditions of 7.5% to 10%. The association of natamycin with sodium chloride potentiates its antimicrobial action, which can represent an economy and its use is amplified by the industries.

show abstract

“…E. coli has been suggested to be capable of growing in media of different nutritional status, and thrive irrespective of the amount of organic matter. This organism is regarded as a more reliable predictor of gastro intestinal illness than other bacterial indicators in water (Goh et al, 2012;How et al, 2013;Wu et al, 2014;Woods, 2015). As a member of the Enterobacteriaceae, the bacteria Escherichia coli is regarded as non-halophilic, and cannot tolerate high salt concentrations (Abdulkarim et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Survival and growth rate of coastal water <i>Escherichia coli</i> isolates in different salt concentrations

Agwu¹,

Oluwagunke²,

Ebohon³

2017

Ife Journal of Science

View full text Add to dashboard Cite

The effect of salt concentration on the survival and growth rate of Escherichia coli isolated from Lagos Lagoon surface water was investigated. This was necessitated to ascertain the suitability of using E. coli as feacal pollution indicator in this water body which experiences fluctuation in salinity values. The salinity during the study ranged from 25.13 and 35.69‰. All the five E. coli isolated during the entire study period, grew luxuriantly in 0 to 4% salt concentrations with increase in optical density values of 78 -93% and high (0.005 -0.45) growth rates within 300 minutes of incubation. The growth of these isolates at 6% salt concentration was also good with 22 -77% optical density increase and growth rate range of 0.25 -0.37. However, minimal or no growth was noted at 8 to 12% salt concentrations with corresponding low increase in optical density and negligible or even negative growth rate. Although the effect of salt concentrations on the five isolates tested were comparable, the isolate (Ec2) which was isolated during the least salinity period, showed the least growth rate in all salt concentrations. These findings suggest that salinity at point of isolation can influence salt tolerance, but further affirms the use of E. coli as pollution indicator for Lagos Lagoon since they exhibited considerable halo-tolerance and survived salt concentrations up to 6%.

show abstract

Adaptation of Escherichia coli ATCC 8739 to 11% NaCl

Cited by 13 publications

References 9 publications

Whole-cell optical biosensor for mercury – operational conditions in saline water

Whole-cell optical biosensor for mercury – operational conditions in saline water

Influence of the salt concentration on action mechanisms of natamycin against microorganisms of importance in food manufacture

Survival and growth rate of coastal water <i>Escherichia coli</i> isolates in different salt concentrations

Contact Info

Product

Resources

About