Adaptation of in vitro regeneration protocol for Brazilian wheat genotypes

Abstract

The availability of an efficient protocol for in vitro regeneration is imperative for genetic transformation. Using genotypes adapted to the target region as a transgenic platform accelerates the development of cultivars. Therefore, this study aimed to adapt an in vitro regeneration protocol for Brazilian wheat genotypes. For this purpose, the in vitro regeneration capacity of immature embryos from six Brazilian wheat genotypes using two protocols of regeneration of somatic embryos was analysed. Furthermore, combinations of 2,4-D and picloram in the callus induction medium were tested in order to improve regeneration efficiency. Genotypes with higher regeneration efficiency were BR18-Terena and PF020037, yielding 0.42 and 1.13 shoots per explant using the Hu and the Wu protocol, respectively. Adding 1mgL-1 2,4-D in the callus induction medium was the most favourable, producing 3.73 and 3.07 shoots per explant for PF020037 and BR18-Terena, respectively. In conclusion, a protocol for regeneration for two Brazilian wheat genotypes recommended and developed to be cultivated at the Cerrado region has been adapted.

Establishment of an efficient regeneration protocol of six different varieties of wheat (Triticum aestivum L.)

“…Various channels of tissue culture were laid out to upgrade callus formation efficiency in wheat which are extensively including genotypic, culture medium, explant dependent and over expression of callus regenerative gene (Roesler et al, 2018;Chaimae et al, 2021;Yu et al, 2023). For all cultivars, new plants, or species using more than one hormone, tissue culture and growth procedures should be investigated through careful design and testing experiments.…”