Adaptation of lactic acid bacteria to unfavorable growth conditions

Golod, N. A.; Лойко, Н. Г.; Mulyukin, A. L.; Neiymatov, A. L.; Vorobjeva, Lena I.; Suzina, N. E.; Shanenko, E. F.; Гальченко, В. Ф.; Эль-Регистан, Г. И.

doi:10.1134/s0026261709030047

Cited by 21 publications

(6 citation statements)

References 15 publications

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“…Similarly, cultivation techniques are limited by readily generating growth of non-fastidious species only on solid primary isolation media. Lactic acid bacteria have well-described inherited adaptation mechanisms for survival in suboptimal conditions, frequently entering a dormant or persistent state, which supports our findings of limited recovery of lactobacilli using traditional culture techniques [ 32 , 33 ]. Larger prospective studies are required to explore the true impact of the fallopian tube microbiota on reproductive health outcomes.…”

Section: Discussionsupporting

confidence: 86%

The fallopian tube microbiome: implications for reproductive health

et al. 2018

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ObjectiveThere is a paucity of data characterizing the microbiota of the female upper genital tract, which controversially is described as a sterile site. We examine whether the fallopian tube harbours an endogenous microbial community.DesignThis prospective study collected from women undergoing total hysterectomy or salpingectomy-oophorectomy.SettingPrivate hospital gynaecology department.PatientsFallopian tubes were collected from women diagnosed with benign disease or for prophylaxis.InterventionsSamples were interrogated for the presence of microbial DNA using a next generation sequencing technology approach to exploit the V5 to V9 regions of the 16S rRNA gene.Main outcome measuresThe fallopian tube microbiota was characterized using traditional culture techniques and next generation sequencing.ResultsBacteria were isolated from 50% of cultured samples, and 100% of samples returned positive PCR results. Only 68% of the culture isolates could be confidently identified using automated diagnostic equipment in a clinical microbiology laboratory. Monomicrobial communities were identified only for cultured isolates (50%). Pyrosequencing revealed that all communities were polymicrobial. Lactobacillus spp. were not present in all groups, nor were they the most dominant isolates. Distinct differences in the microbial communities were evident for left compared to right fallopian tubes, ampulla versus isthmus, pre- and post- menopausal tissue, and in secretory phase fallopian tubes with and without Mirena intrauterine devices in situ (all p < 0.05).ConclusionThe female upper genital tract is not sterile. Distinct microbial community profiles in the fallopian tubes of healthy women suggest that this genital tract site supports an endogenous microbiota.

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Section: Discussionsupporting

confidence: 86%

The fallopian tube microbiome: implications for reproductive health

et al. 2018

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“…Viability of exponential phase cells of both the WCFS1 and the WCFS1-2 slowly decreased during storage, while stationary phase cells showed a much better survival. Evidence of entry in a VBNC status has been found for L. plantarum (Golod et al, 2009) with formation of cyst like dormant structures in some conditions. In our experimental set-up a temperature downshift to 4 °C (close to the minimum temperature for growth for L. plantarum, Zwietering et al, 1991) and storage in a nutrient depleted buffer provide both a cold and starvation stress.…”

Section: Locusmentioning

confidence: 97%

Inactivation of ccpA and aeration affect growth, metabolite production and stress tolerance in Lactobacillus plantarum WCFS1

Zotta

Ricciardi

Guidone

et al. 2012

International Journal of Food Microbiology

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“…Prior to inoculation, media were filtered through Millex ® ‐GV 0.22 μm filters (Millipore). Microbial growth after incubation at 37 °C under anaerobic conditions was evaluated by both turbidimetric measurement (Golod and others 2009) and microscopic count. A dilution step was performed before measuring the optical density (OD) of solution with an OD value greater than 1.8.…”

Section: Methodsmentioning

confidence: 99%