Adaptation of Lactobacillus alimentarius to environmental stresses

Lemay, Marie-Josée; Rodrigue, N.; Gariépy, C.; Saucier, Linda

doi:10.1016/s0168-1605(00)00181-1

Cited by 25 publications

(8 citation statements)

References 4 publications

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“…In fact, it took recombinant L. casei/L1 clones 12 h longer than it took the wild-type strain to reach an optical density of 2.0 (data not shown). Studies with Lactobacillus strains revealed cell filamentation in a Lactobacillus plantarum cell wall mutant (29) and in Lactobacillus alimentarius subjected to stress after treatment with organic acids (20). However, to our knowledge there are no published data about cell elongation during the production of recombinant proteins in Lactobacillus.…”

Section: Discussionmentioning

confidence: 98%

Production of Human Papillomavirus Type 16 L1 Virus-Like Particles by RecombinantLactobacillus caseiCells

Aires

Cianciarullo

Carneiro

et al. 2006

Appl Environ Microbiol

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Infections with human papillomavirus type 16 (HPV-16) are closely associated with the development of human cervical carcinoma, which is one of the most common causes of cancer death in women worldwide. At present, the most promising vaccine against HPV-16 infection is based on the L1 major capsid protein, which self-assembles in virus-like particles (VLPs). In this work, we used a lactose-inducible system based on the Lactobacillus casei lactose operon promoter (plac) for expression of the HPV-16 L1 protein in L. casei. Expression was confirmed by Western blotting, and an electron microscopy analysis of L. casei expressing L1 showed that the protein was able to self-assemble into VLPs intracellularly. The presence of conformational epitopes on the L. casei-produced VLPs was confirmed by immunofluorescence using the anti-HPV-16 VLP conformational antibody H16.V5. Moreover, sera from mice that were subcutaneously immunized with L. casei expressing L1 reacted with Spodoptera frugiperda-produced HPV-16 L1 VLPs, as determined by an enzymelinked immunosorbent assay. The production of L1 VLPs by Lactobacillus opens the possibility for development of new live mucosal prophylactic vaccines.

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Section: Discussionmentioning

confidence: 98%

Production of Human Papillomavirus Type 16 L1 Virus-Like Particles by RecombinantLactobacillus caseiCells

Aires

Cianciarullo

Carneiro

et al. 2006

Appl Environ Microbiol

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“…Cell filamentation is observed in many bacteria under stress (29,36,46,63), probably as a result of the normal stress response. In our experiments, control strains expressing a functional HtrA BCAL2829 formed long filaments under salt stress conditions, consistent with previous observations of NaClstressed Salmonella and Listeria (16,20).…”

Section: Discussionmentioning

confidence: 99%

Burkholderia cenocepacia Requires a Periplasmic HtrA Protease for Growth under Thermal and Osmotic Stress and for Survival In Vivo

Flannagan¹,

Aubert²,

Kooi

et al. 2007

Infect Immun

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Burkholderia cenocepacia, a member of the B. cepacia complex, is an opportunistic pathogen that causes serious infections in patients with cystic fibrosis. We identified a six-gene cluster in chromosome 1 encoding a two-component regulatory system (BCAL2831 and BCAL2830) and an HtrA protease (BCAL2829) hypothesized to play a role in the B. cenocepacia stress response. Reverse transcriptase PCR analysis of these six genes confirmed they are cotranscribed and comprise an operon. Genes in this operon, including htrA, were insertionally inactivated by recombination with a newly created suicide plasmid, pGP⍀Tp. Genetic analyses and complementation studies revealed that HtrA BCAL2829 was required for growth of B. cenocepacia upon exposure to osmotic stress (NaCl or KCl) and thermal stress (44°C). In addition, replacement of the serine residue in the active site with alanine (S245A) and deletion of the HtrA BCAL2829 PDZ domains demonstrated that these areas are required for protein function. HtrA BCAL2829 also localizes to the periplasmic compartment, as shown by Western blot analysis and a colicin V reporter assay. Using the rat agar bead model of chronic lung infection, we also demonstrated that inactivation of the htrA gene is associated with a bacterial survival defect in vivo. Together, our data demonstrate that HtrA BCAL2829 is a virulence factor in B. cenocepacia.

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“…However, in most studies this effect was observed when cells were subjected to an acid shock and not by growing cells under noncontrolled pH (O'Driscoll et al. 1996; O'Sullivan and Condon 1997; Lemay et al. 2000; Lim et al.…”

Section: Discussionmentioning

confidence: 99%

Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying

et al. 2005

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Aims: The aim of this study was to optimize survival of Lactobacillus delbrueckii subsp. bulgaricus during spraydrying and subsequent storage through optimizing the pH of growth conditions. Methods and Results: Cell concentrates previously grown without or with pH controlled were spray-dried and stored at 20°C and heat treated at 57°C. Cells grown under noncontrolled pH were more resistant to both drying and heating than cells grown under controlled pH but no significant differences were observed during storage. The intracellular proteins profile of cells grown under both conditions was studied by two-dimensional SDS-polyacrylamide gel electrophoresis. Eight proteins were identified using automated mass spectrometry (MS) and tandem mass spectrometry (MS/MS) data acquisition. Of the identified proteins, only cochaperonin GroES corresponded to a known heat shock protein (HSP). The other proteins identified are proteins involved in glycolysis. For cells grown under noncontrolled pH the expression of the Hsp70, GroES and GroEL, measured by Western blotting, was enhanced. Conclusions: The higher resistance of cells grown under noncontrolled pH correlates with the enhanced production of heat shock proteins. Significance and Impact of the Study: Growth of L. bulgaricus under controlled pH (commonly used by the starter cultures production industry) results in cells more sensitive to stresses frequently encountered by the cells during starter cultures preparation/storage/utilization.

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Adaptation of Lactobacillus alimentarius to environmental stresses

Cited by 25 publications

References 4 publications

Production of Human Papillomavirus Type 16 L1 Virus-Like Particles by RecombinantLactobacillus caseiCells

Production of Human Papillomavirus Type 16 L1 Virus-Like Particles by RecombinantLactobacillus caseiCells

Burkholderia cenocepacia Requires a Periplasmic HtrA Protease for Growth under Thermal and Osmotic Stress and for Survival In Vivo

Effect of the pH of growth on the survival of Lactobacillus delbrueckii subsp. bulgaricus to stress conditions during spray-drying

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