Adaptation of Mammalian Cells to Growth in Serum-Free Media

Improving the secretory capacity of Chinese hamster ovary cells by ectopic expression of effector genes: Lessons learned and future directions

“…Besides being the host cell used for the first approval of a recombinant biopharmaceutical produced in mammalian cells in 1986 (Wurm, 2004), CHO cells are the preferred choice for a number of reasons. First, CHO cells can easily be adapted for highdensity suspension growth in a chemically defined, serum-free medium in large-volume cultures (Kim et al, 2012;Sinacore et al, 2000). Second, gene amplification methods have been established for CHO cells, leading to high specific productivity (q p ) of recombinant protein (r-protein) in stable cell lines (Durocher and Butler, 2009).…”

Metabolomics and Mammalian Cell Culture

“…NS0 cells capable of cholesterol-free growth can be isolated by selecting mutant clones or by adaptation. Adaptation generally involves passaging cells over a time period during which the serum concentration is decreased gradually (Sinacore et al, 2000). Eventually, the resulting population develops the capability to grow in the absence of serum.…”

Anoikis-resistant MDCK cells carrying susceptibilities to TNF-α and verotoxin that are suitable for influenza virus cultivation

“…A suspension culture of this cell line has been devised for the large-scale production of the influenza virus by introducing solid carriers to the medium (Tree et al 2001;Merten et al 1996) or by culturing the cells with shaking. The latter is a typical culture method recommended for the MDCK cells to adapt to growth in a non-adherent suspension (Sinacore et al 2000;Valley et al 2006). However, the mechanisms underlying the transition of the proliferation status of this cell type from matrixadherent to suspension are not fully understood and are under investigation in terms of the interplay between integrin signaling and apoptosis signaling pathways including focal adhesion kinase (FAK) and Rho family GTPases (Frisch et al 1996;Cheng et al 2004).…”