Adaptation of Saccharomyces cerevisiae to high pressure (15, 25 and 35 MPa) to enhance the production of bioethanol

Optimization of Gamma Aminobutyric Acid Production Using High Pressure Processing (HPP) by Lactobacillus brevis PML1

“…Ferreira et al [ 37 ] studied the effect of pressure at three levels on Saccharomyces cerevisiae to raise its bioethanol production efficiency. They exerted 15, 25, and 35 MPa on the yeast at 30°C for 72 h and observed that ethanol was most produced at 25 MPa.…”

Cellular NADH and NADPH Conformation as a Real-Time Fluorescence-Based Metabolic Indicator under Pressurized Conditions

“…Deep-ocean pressures can exceed 100 MPa, and so the pressures used here are relevant to life [ 5 , 6 , 7 , 8 ]. Although a change of tens of MPa pressure is not a typical physiological range for S. cerevisiae , it may encounter these changes during food or biotechnological processing [ 18 ]. On the other hand, these pressure changes may be physiological for organisms inhabiting high-pressure environments, and so S. cerevisiae serves as a model system for piezophysiology [ 6 ].…”

“…The observation of a cycle-dependent autofluorescence response ( Figure 2 ) shows promise for this line of investigation. Next, because pressure affects fermentation [ 18 ], we decided to investigate the autofluorescence response to ethanol. The observation of a pressure-dependent autofluorescence response to ethanol ( Figure 5 and Figure 6 ) suggests the potential for future work in this area as well.…”

“…Information on NADH- and NADPH-linked metabolism under pressure also has applications in agriculture and food technologies. For example, real-time monitoring of NADH may be useful in bioethanol production, which is influenced by pressure [ 18 ] or in improving the flavor and quality of brewed products because high NADH availability is a factor for maintaining low acetaldehyde content during alcoholic fermentation [ 19 , 20 ]. With the usefulness of pressure as a biophysical tool being well recognized [ 8 , 21 , 22 , 23 ], extending techniques for the real-time monitoring of cellular NADH and NADPH conformation to pressurized conditions provides a label-free approach for investigating a range of NADH- and NADPH-linked metabolic function.…”

Impact of Fermentation and Pasteurization on the Physico-Chemical and Phytochemical Composition of Opuntia ficus-indica Juices

“…A culture was prepared by inoculating a single colony in 100 mL of sterile culture medium (yeast malt broth) containing 5 g/L animal tissue peptide digest (peptone), 3 g/L yeast extract, 3 g/L malt extract, and 10 g/L dextrose, with the pH adjusted to 4 using 1 M citric. The culture was then incubated at 30 • C with constant agitation at 150 rpm for a duration of 36 h. The inoculum was considered ready for use when the optical density of the culture medium reached 0.8, as measured at a wavelength of 600 nm [23].…”