Adaptation of the Acidophilic Fungus Sistotrema brinkmannii to the pH Factor

Ianutsevich, Elena A.; Данилова, О. А.; Grum-Grzhimaylo, Olga A.; Groza, N. V.; Терешина, В. М.

doi:10.1134/s0026261723600210

Cited by 5 publications

(13 citation statements)

References 44 publications

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“…In our studies, we have shown that PAs, which are minor components in non-extremophiles [ 44 ], are present in extremophiles, either as predominant or as one of the main membrane lipids [ 24 , 25 , 45 , 46 , 47 ]. PAs are multifunctional and poorly studied compounds, being a key metabolite of lipogenesis and performing regulatory and structural functions [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

“…It is assumed that PAs can control transport from the Golgi apparatus and EPR, endocytosis, and exocytosis due to the ability to aggregate into microdomains, which leads to bending of membranes, resulting in vesicle formation. Previously, we have shown for the first time a high proportion of PA in various extremophilic fungi: thermophiles [ 45 ], alkaliphiles [ 46 ], acidophiles [ 24 , 25 ], and xerophiles [ 47 ]. Acidophiles, unlike neutrophils, are also characterized by the presence of PA as one of the main phospholipids, along with PE, PC, and sterols, which also confirms their role in extremophilia [ 24 , 25 ].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we have shown for the first time a high proportion of PA in various extremophilic fungi: thermophiles [ 45 ], alkaliphiles [ 46 ], acidophiles [ 24 , 25 ], and xerophiles [ 47 ]. Acidophiles, unlike neutrophils, are also characterized by the presence of PA as one of the main phospholipids, along with PE, PC, and sterols, which also confirms their role in extremophilia [ 24 , 25 ]. These data suggest that extremophile membranes have a special structure compared with non-extremophile membranes.…”

Section: Discussionmentioning

confidence: 99%

“…Lipids, carbohydrates, and polyols analyses were performed as described earlier [ 24 ]. Briefly, lipids were extracted by the Nichols method [ 52 ] with phospholipase-deactivating isopropanol, separated using two-dimensional (polar lipids) or one-dimensional (neutral lipids) thin-layer chromatography (TLC) [ 53 ] and quantified using standard compounds by densitometry method (DENS software, version 5.1.0.2).…”

Section: Methodsmentioning

confidence: 99%

“…Acidophiles, like all other fungi, are exposed to additional stress factors (heat, cold, osmotic) under natural conditions. Previous data on the effects of trehalose protection [ 24 , 25 ] suggest that acidophiles can use the cell membrane and osmolytic systems to adapt to a variety of abiotic stressors.…”

Section: Introductionmentioning

confidence: 99%

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Membrane Lipids and Osmolytes in the Response of the Acidophilic Basidiomycete Phlebiopsis gigantea to Heat, Cold, and Osmotic Shocks

Ianutsevich,

Danilova,

Grum-Grzhimaylo

et al. 2024

IJMS

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Previously, we found for the first time the participation of osmolytes in adaptation to acidic conditions in three acidophilic fungi. Because trehalose can protect membranes, we hypothesized a relationship between osmolyte and membrane systems in adaptation to stressors. In the mycelium of Phlebiopsis gigantea, the level of osmolytes reaches 8% of the dry mass, while trehalose and arabitol make up 60% and 33% of the sum, respectively. Cold shock does not change the composition of osmolytes, heat shock causes a twofold increase in the trehalose level, and osmotic shock leads to a marked increase in the amount of trehalose and arabitol. Predominance of phospholipids (89% of the sum) and low proportions of sterols and sphingolipids are characteristic features of the membrane lipids’ composition. Phosphatidic acids, along with phosphatidylethanolamines and phosphatidylcholines, are the main membrane lipids. The composition of the membrane lipids remains constant under all shocks. The predominance of linoleic (75% of the sum) and palmitic (20%) acids in phospholipids results in a high degree of unsaturation (1.5). Minor fluctuations in the fatty acid composition are observed under all shocks. The results demonstrate that maintaining or increasing the trehalose level provides stability in the membrane lipid composition during adaptation.

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