Adaptation of the enzyme-linked immunosorbent assay (ELISA) to the quantitative analysis of abscisic acid

Daie, Jaleh; Wyse, Rogér E.

doi:10.1016/0003-2697(82)90599-1

Cited by 49 publications

(26 citation statements)

References 7 publications

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“…The data ( Table 1, Figures 1, 2) show high specificity and selectivity of the ABA-mAb as compared with previous studies [4,8,14,241. This most likely results from the use of mAb rather than polyclonal sera, which contain also low affinity Ab that are probably responsible for non-specific cross reactions.…”

Section: Discussionsupporting

confidence: 72%

“…This included generations of a new ABA-mAb and development of an indirect ELISA [20], which provides substantial amplification of the response. To our knowledge, this combination of mAb with an indirect ELISA has been applied only few times before for ABA quantitation [2,9,19,221, while all other studies dealing with immunoassays for ABA used polyclonal Ab [4,8,14,24,251 or mAb coupled with RIA [15,18,211 rather than with ELISA. Employing these immunological techniques minimized the need for purification of the ABA extracts, so that a large number of samples could be processed with little procedural effort, and correspondingly little sample loss.…”

Section: Discussionmentioning

confidence: 99%

“…The most commonly used methods for ABA quantification are by HPLC-UV, followed by gas liquid chromatography (GLC) These techniques require large quantities of tissue, as well as rigorous clean-up procedures to ensure elimination of contaminants that may cochromatograph with ABA [16]. The introduction of mAb as reagents for analyses of plant hormones [26] has provided attractive alternatives to the traditional assays of ABA, which minimize the need for purification and allow rapid analysis of many samples with minimal costs [4,8,9,14,15,24,251. While the list of immunoassays being reported for ABA analysis in leaves and other tissues is increasing rapidly [2, 4, 8, 9, 14, 16, 19, 24, 251, there are as yet only a few reports of new monoclonal antibodies (mAbs) to ABA [15,17,18,21,221.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, we have developed a new mAb for ABA and employed a sensitive enzyme-linked immunosorbent assay (ELISA) system [20]. Unlike previous ELISA quantitations of ABA, which coupled the hormone with alkaline phosphatase [4,9,251, this report describes an indirect ELISA method, that provides substantial amplification of the response by employing a second antibody, goat anti-mouse, coupled to the enzyme horse-radish peroxidase (HRP) [20]. A similar approach is now used by several other groups [19,2,221.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves

1993

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A new monoclonal antibody (mAb) was generated against abscisic acid (ABA), and an indirect enzymelinked immunosorbent assay (ELISA) using this mAb was developed for convenient quantitative analysis of ABA levels in rice leaf extracts. The mAb, raised against (+)-ABA conjugated to bovine serum albumin (BSA) through its carboxyl group (C,), reacted preferentially with the (+)-ABA enantiomer, and equally well with both free and methyl-ester (+)-ABA. Cross-reactivity with several ABA-related compounds was negligible. Linearity was obtained between 3 and 1OOOpmol of (+)-ABA. The ABA-mAb was further used to quantitate pmol quantities of (+)-ABA in attached and detached rice leaves. Results obtained with such ELISA quantitation showed an increase in the free ABA content of detached rice leaves at progressive stages of senescence, which was regarded as a senescence-related response. This quantitation compared favorably with other presently used techniques for ABA determination, with regard to their detection limits, cost and assay time. The results suggest that the combination of a specific mAb with a sensitive ELISA technique is quite promising for quantitation of ABA.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves

1993

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“…Antibodies against ABA were prepared by conjugating (±)-ABA to bovine serum albumin (BSA) through the -COOH group [17] . Immunoglobulin G (IgG) of rabbit antisera was isolated using protein-A affinity chromatography according to the method of Ey et al .…”

Section: Aba Determinationmentioning

confidence: 99%

Abscisic acid and mefluidide in the regulation of cold hardiness development in Solanum tuberosum L. potato

Costa

Ryu

1993

Plant Growth Regul

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Plants of Solanum tuberosum L . potato do not cold acclimate when exposed to low temperature such as 5 °C, day/night .When ABA (45 µM) was added to the culture medium, stem-cultured plantlets of S. tuberosum, cv . Red Pontiac, either grown at 20'C/15'C, day/night, or at 5'C, increased in cold hardiness from-2'C (killing temperature) to -4.5'C . The increase in cold hardiness could be inhibited in both temperature regimes if cycloheximide (70 pM) was added to the culture medium at the inception of ABA treatment . Cycloheximide did not inhibit cold hardiness development, however, when it was added to the culture medium 3 days after ABA treatment .When pot-grown plants were foliar sprayed with mefluidide (50 pM), ABA content increased from 10 nmol to 30 nmol g-1 dry weight and plants increased in cold hardiness from-2'C to about -3.5'C .The increases in free ABA and cold hardiness occurred only in plants grown at 20'C/15'C ; neither ABA nor cold hardiness increased in plants grown at 5'C . The results suggest that an increase in ABA and a subsequent de novo synthesis of proteins are required for the development of cold hardiness in S . tuberosum regardless of temperature regime, and that the inability to synthesize ABA at low temperature, rather than protein synthesis, appears to be the reason why S . tuberosum does not cold acclimate . 303

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Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole‐3‐acetic acid in plants

Zhang

Jin

et al. 2008

Phytochemical Analysis

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Adaptation of the enzyme-linked immunosorbent assay (ELISA) to the quantitative analysis of abscisic acid

Cited by 49 publications

References 7 publications

Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves

Characterization and use in ELISA of a new monoclonal antibody for quantitation of abscisic acid in senescing rice leaves

Abscisic acid and mefluidide in the regulation of cold hardiness development in Solanum tuberosum L. potato

Study on the extraction, purification and quantification of jasmonic acid, abscisic acid and indole‐3‐acetic acid in plants

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