Adaptation of the FK506 binding protein 1B to hibernation in bats

Unexpected expression of heat-activated transient receptor potential (TRP) channels in winter torpid bats and cold-activated TRP channels in summer active bats

“…We found that torpid and 2 h-aroused bats had lower rectal temperatures than 24 h-aroused and summer active bats. This is consistent with previous findings that the metabolic strategy used by torpid bats is similar to that of 2 h-aroused bats, and that used by 24 h-aroused bats is similar to that of summer active bats ( Huang et al, 2020 ; Liu et al, 2018 ). We also found that all bat groups and all mouse groups had a basal expression level of various Thermo-TRPs ( Figures 1A , 3A), suggesting that these animals constantly sense and respond to temperature variations.…”

“…These sequences were scanned separately using the matrix-scan tool on the RSAT webserver (http://rsat.sb-roscoff.fr/matrix-scan_form.cgi) for TF binding sites. As each TF has its own PFM, the number of TF binding sites in each sequence was calculated at different threshold values ( P= 10 −5 to 10 −8 ) (Supplementary Excel File 1) ( Liu et al, 2018 ). The binding sites found in the gene sequences of hibernating, non-hibernating, or both species of mammals were labeled as H, NH, and B, respectively (Supplementary Excel File 2).…”

“…In the chart, bars representing the state with the highest gene mRNA abundance were marked "a", and bars representing the states with the second and third highest and lowest abundance were marked "b", "c", and "d", respectively. In addition, significant differences ( P <0.05) in values were denoted with different letters ( Huang et al, 2020 ; Liu et al, 2018 ; Yin et al, 2016 ). In the small bar chart of TRPV2 ( Figure 1A ), the bar labeled “bc” indicates that the state had a significantly lower mRNA abundance than the state represented by the bar labeled “a,” but showed no significant difference with the state represented by the bar labeled “b” or “c”.…”