Adaptation of the herbicide-degrading strain delftia acidovorans mc1 through carbonylation of rdpa as key enzyme

Leibeling, Sabine; Taubert, Martin; Seifert, Jana; Bergen, Martin von; Harms, Hauke; Müller, Roland

doi:10.1016/j.jbiotec.2010.09.160

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bacteria of the genus Delftia mediate a diversity of processes important in environmental toxicology, including xenobiotic biodegradation and biotransformation of heavy metals (Vacca et al, 2005 ; De Gusseme et al, 2010 ; Juarez-Jimenez et al, 2010 ; Leibeling et al, 2010 ; Paulin et al, 2010 ; Zhang et al, 2010 ; Morel et al, 2011 ; Yang et al, 2011 ). Additionally, Delftia spp.…”

Section: Introductionmentioning

confidence: 99%

Development of Tools for Genetic Analysis of Phenanthrene Degradation and Nanopod Production by Delftia sp. Cs1-4

Chen¹,

Hickey²

2011

Front. Microbio.

View full text Add to dashboard Cite

The bacterium Delftia sp. Cs1-4 produces novel extracellular structures (nanopods) in conjunction with its growth on phenanthrene. While a full genome sequence is available for strain Cs1-4, genetic tools that could be applied to study phenanthrene degradation/nanopod production have not been reported. Thus, the objectives of this study were to establish such tools, and apply them for molecular analysis of nanopod formation or phenanthrene degradation. Three types of tools were developed or validated. First, we developed a new expression system based on a strong promoter controlling expression of a surface layer protein (NpdA) from Delftia sp. Cs1-4, which was ca. 2,500-fold stronger than the widely used lactose promoter. Second, the Cre-loxP system was validated for generation of markerless, in-frame, gene deletions, and for in-frame gene insertions. The gene deletion function was applied to examine potential roles in nanopod formation of three genes (omp32, lasI, and hcp), while the gene insertion function was used for reporter gene tagging of npdA. Lastly, pMiniHimar was modified to enhance gene recovery and mutant analysis in genome-wide transposon mutagenesis. Application of the latter to strain Cs1-4, revealed several new genes with potential roles in phenanthrene degradation or npdA expression. Collectively, the availability of these tools has opened new avenues of investigation in Delftia sp. Cs1-4 and other related genera/species with importance in environmental toxicology.

show abstract