Sustainable development can be achieved through several activities. The building and construction sector (B & C sector) is one of the major industries, and it can play a crucial role in the improvement of the most relevant environmental impacts. Nowadays, there are major concerns related to sustainability in construction. All types of buildings have different technical aspects; therefore, it is required to develop specific sustainability assessment tools. A school building has peculiarities connected to aspects of sustainability, as it is a building planned to offer adequate environments for the education of adolescents and children. This article shows the application of the SBTool methodology developed specifically for Portuguese high schools, SAHSBPT (Sustainable Assessment for High School Buildings) methodology, that is being elaborated by the first author in her PhD Thesis. This methodology allows architects, engineers and designers to improve sustainability in school buildings, in projects or in the rehabilitation of buildings. The objective of this research is to apply that evaluation tool in order to verify the efficiency of this methodology, as well as to recognize the level of sustainability of the Francisco de Holanda High School Building, in Guimarães, Portugal. The values found in the application of the Sustainable Assessment for High School Buildings in that high schools demonstrate a good result, as the overall value is A, 75% of the total result.