Adaptation of trypsin, chymotrypsin and α-amylase to casein level and protein source in Penaeus vannamei (Crustacea Decapoda)

Moullac, Gilles Le; Klein, Birgit; Sellos, Daniel; Wormhoudt, Alain Van

doi:10.1016/s0022-0981(96)02671-8

Cited by 149 publications

(110 citation statements)

References 52 publications

Supporting

Mentioning

100

Contrasting

Unclassified

Order By: Relevance

“…Le Moullac et al 1996;Jones et al 1997;Kumlu and Jones 1997;Le Vay et al 2001). They appear at high activities in the midgut gland and, particularly, in the stomach where they facilitate the extra-cellular cleavage of alimentary proteins (Dall and Moriarty 1983 and literature cited therein).…”

Section: Discussionmentioning

confidence: 99%

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

et al. 2006

View full text Add to dashboard Cite

The early ontogenetic stages of the sub-Antarctic king crab Lithodes santolla were analyzed for the presence and activities of a set of important digestive enzymes. The eggs and non-feeding larvae (zoea I-III, megalopa) showed high activities of esterases, phosphatases, and exopeptidases indicating the enzymatic ability to utilize endogeneous yolk reserves. SDS-PAGE showed a continuous decrease of proteins or proteids in the range of 59-81 kDa during ontogenetic development from the eggs through the zoeal stages to the first juvenile crab stage, CI. This reduction reflects the degradation of storage compounds during lecithotrophic larval development. Activities of the endopeptidases, trypsin and chymotrypsin, were low in eggs and larvae but increased significantly in the first juvenile crab stage. These enzymes typically facilitate the first steps of proteolysis in the extra-cellular spaces of the midgut gland and in the stomach. Their scarcity indicates that the larvae of L. santolla are physiologically not prepared to digest external food. This ability seems to appear first in the CI stage. Extracts of juvenile midgut glands and the gastric fluids of adults showed high activities of a variety of digestive enzymes including phosphatases, carbohydrases, as well as endo-and exopeptidases. High activities of digestive enzymes in adults may compensate for scarce food supply and rate-limiting low temperatures in the predominantly sub-Antarctic habitats of L. santolla.

show abstract

Section: Discussionmentioning

confidence: 99%

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

et al. 2006

View full text Add to dashboard Cite

show abstract

“…It is synthesised in large quantities and may represent up to one-third of the soluble proteins in the digestive gland (Ceccaldi, 1998). Many investigations confirmed high trypsin activities in different crustacean species (e.g., Murhty and Saxena, 1979;Garcia-Carreño et al, 1994Johnston et al, 1995;Hernández-Cortés et al, 1997;Le Moullac et al, 1997). However, preliminary but repeated enzyme analysis of the midgut glands of C. crangon as well as C. allmani showed unusually low or even no trypsin activities, while in the same samples, total proteolytic activities were high (Saborowski, unpublished).…”

Section: Introductionmentioning

confidence: 99%

Cysteine proteinases substitute for serine proteinases in the midgut glands of Crangon crangon and Crangon allmani (Decapoda: Caridea)

Teschke

Saborowski

2005

Journal of Experimental Marine Biology and Ecology

View full text Add to dashboard Cite

The utilization of dietary proteins in crustaceans is facilitated by a set of peptide hydrolases which are often dominated by btrypsin-likeQ serine proteinases. As expected, the North Sea shrimps Crangon crangon and Crangon allmani showed in their midgut glands high proteolytic activities. However, the majority of animals lacked trypsin and chymotrypsin. Conversely, a minority of about 10% of the animals had elevated trypsin activities. The appearance of trypsin was neither related to the mode of feeding nor to the nutritive state of the animals. When present, trypsin was expressed in both species as a single isoform of apparently 20 kDa. The lack of serine proteinases was also confirmed by inhibitor assays. AEBSF, a serine proteinase inhibitor, slightly reduced total proteinase activity by less than 10%. In contrast E 64, a cysteine proteinase inhibitor, caused a reduction of more than 70% of total proteinase activity, indicating that a substantial share of proteolytic activity is caused by cysteine proteinases. Cathepsin L-like proteinases were identified as major cysteine proteinases.A comparison with the eucarid crustaceans Pandalus montagui, Pagurus bernhardus, Cancer pagurus and Euphausia superba showed a similar high level of total proteinase activity in all species. Trypsin, however, varied significantly between species showing lowest activities in Caridea and the highest activity in E. superba. E 64 suppressed total proteinase activity by more than 70% in Crangon species but not in C. pagurus and E. superba. In contrast, the serine proteinase inhibitor AEBSF had only little effect in Caridea but was most effective in P. bernhardus, C. pagurus and E. superba. The results may indicate different traits of food utilization strategies in some eucarid crustaceans. Caridea may express predominantly cysteine proteinase, while in Anomura, Brachyura and Euphausiacea, serine proteinases may prevail. D

show abstract

“…The applications of microalgae for aquaculture are associated with nutrition and other biological activities (Hemaiswarya et al, 2011). The study of digestive enzymes constitutes a denominator role to consider the nutritional requirements of specific stages of development in shrimps (Le Moullac et al, 1997). In the present study, the zoea to postlarvae 20 of Penaeus monodon and Litopenaeus vannamei were fed with five different microalgae such as, Isochrysis galbana, Cheatoceros calcitrans, Tetraselmis sp., Chlorella sp.…”

Section: Resultsmentioning

confidence: 96%

Attenuation of Negative Impacts by Micro Algae and Enriched Artemia salina on Penaeus monodon and Litopenaeus vannamei Larval Culture

Karthik¹,

Pushpam²,

Kirubagaran³

et al. 2015

J. of Fisheries and Aquatic Science

View full text Add to dashboard Cite

Shrimp farming is one of the most important aquaculture and economically lucrative practices of maritime countries. Providing a Specific Pathogen Free (SPF) shrimps to the farmers is a big challenge that must be addressed to meet the demand. In general, microalgae are utilized in aquaculture as a live feed for the shrimps. However, their importance in the attenuation of negative impacts of pathogenic microbial load, eutrophication and promotion of shrimps growth has to be delineated by experimental investigations to justify the above specific pathogen free shrimps. The present study was carried out to investigate the feeding of five different microalgae and algae enriched Artemia salina. Nauplii on digestive enzyme activity, growth, survival rate, microbial load on Penaeus monodon and Litopenaeus vannamei from zoea to post larvae (20 stages) and other water quality. Microalgae such as Isochrysis galbana, Cheatoceros calcitrans, Tetraselmis sp., Chlorella sp. and Nannochloropsis sp. were obtained from AMET Microbial Culture Collection Centre, Department of Marine Biotechnology, AMET University. Penaeus monodon and Litopenaeus vannamei at PL 20 stage showed maximum protease and amylase (digestive enzyme) activity, maximum length and survival rate when fed with Artemia salina nauplii enriched with C. calcitrans followed by Chlorella sp. On studying the water quality parameters such as, pH, temperature, salinity, dissolved oxygen and ammonia it was found better in tank II where the shrimps were fed with Artemia salina enriched with C. calcitrans. Regarding the vibrio load at different stages of P. monodon and L. vannamei larvae and cultured water it was comparatively lower in tank II, where the shrimps were fed with Cheatoceros calcitrans and enriched Artemia salina than other groups. It is concluded that, the use of Cheatoceros calcitrans and enriched Artemia salina will reduce the potential negative impacts in the environment and promote production of shrimp larvae in hatcheries and fetch benefits to local economies.

show abstract

Adaptation of trypsin, chymotrypsin and α-amylase to casein level and protein source in Penaeus vannamei (Crustacea Decapoda)

Cited by 149 publications

References 52 publications

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

Digestive enzymes in the ontogenetic stages of the southern king crab, Lithodes santolla

Cysteine proteinases substitute for serine proteinases in the midgut glands of Crangon crangon and Crangon allmani (Decapoda: Caridea)

Attenuation of Negative Impacts by Micro Algae and Enriched Artemia salina on Penaeus monodon and Litopenaeus vannamei Larval Culture

Contact Info

Product

Resources

About