1983
DOI: 10.1099/0022-1317-64-4-853
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Adaptation of Two Avian Rotaviruses to Mammalian Cells and Characterization by Haemagglutination and RNA Electrophoresis

Abstract: SUMMARYTurkey and chicken rotaviruses were successfully adapted to replicate in a rhesus monkey embryo kidney (MA104) cell line. Trypsin treatment of virus and cells was essential for serial passage of the viruses. Polyacrylamide gel electrophoresis separated the viral RNA into 11 segments with clear resolution of segments 10 and I 1. Segment 5 migrated with size class I, and this characteristic appeared to be a unique feature of the avian rotavirus genome. The ability of the avian rotaviruses to haemagglutina… Show more

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Cited by 13 publications
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