Adaptations of Escherichia coli K 12 to Synchrotron Sourced THz Radiation

Abstract

The biological effects of electromagnetic field (EMF) irradiation in the terahertz (THz) range remain ambiguous, despite numerous studies that have been conducted. In this paper, the metabolic response of Escherichia coli K 12 to EMF irradiation was examined using a 1.0 W m −2 incident synchrotron source (SS) in the range of 0.5−18.0 THz for over 90 min of continuous exposure at 25 °C. This continuous SS THz exposure induced periodic decreases in the cell growth after 10, 20, and 40 min of exposure compared to a time-matched control; however, the number of viable cells thereafter grew. The physiological status of treated cells immediately after exposure was assessed by using the direct plate counting technique and electron microscopy. Analysis of scanning electron microscopy (SEM) and high-resolution cryogenic transmission electron (cryo-TEM) micrographs showed that approximately 20% of the SS THz-exposed E. coli cells exhibited a deformed outer membrane, membrane perturbations, and leakage of cytosol. The proteome changes in E. coli cells after 18 h postexposure were associated with cellular response to plasma membrane regulation including phospholipid biosynthetic process and osmotic stress. The results of this study highlighted that E. coli cells can promptly activate the fundamental mechanisms in response to prolonged exposure to THz radiation that are evolutionarily developed to withstand other environmental stressors.