“…However, from those studies it is clear that a number of transport-related factors influence horse welfare. Journey distance and time [20,21], loading density [7,15,22,23], handling [7,24,25], new environments [24], potentially re-grouping or mixing with unfamiliar animals [24], fasting and deprivation of water [24], the myriad of trailer designs [7,23], driving behaviour [7], road type and quality [7,22,24], traffic conditions [24], suspension systems and building materials of the trailers [15,23], environmental conditions in the trailer [7,22], and weather conditions [15,23,26] all affect horse welfare.…”