2017
DOI: 10.1101/205799
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Adapterama III: Quadruple-indexed, double/triple-enzyme RADseq libraries (2RAD/3RAD)

Abstract: Keywords: 3RAD, dual digest RADseq, ddRAD, DNA libraries, multiplexing, NGS. Bldg., University of Georgia, Athens, GA 30602, USA; 3 2 E-mail: njbayona@uga.edu . CC-BY-NC-ND 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/205799 doi: bioRxiv preprint first posted online Oct. 19, 2017; 2 Running Title: 3RAD -tri… Show more

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Cited by 38 publications
(57 citation statements)
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“…Our results also suggest that hybrid females are not selective of parental species in their mate choice. The reduced-representation genomic data obtained with a 3RAD approach 42 confirmed the introgression level of some backcrossed hatchlings using 2,405 3RAD loci. This is the first time that backcrosses with loggerhead turtles are confirmed by genome-wide sequencing analysis.…”
Section: Discussionsupporting
confidence: 55%
See 1 more Smart Citation
“…Our results also suggest that hybrid females are not selective of parental species in their mate choice. The reduced-representation genomic data obtained with a 3RAD approach 42 confirmed the introgression level of some backcrossed hatchlings using 2,405 3RAD loci. This is the first time that backcrosses with loggerhead turtles are confirmed by genome-wide sequencing analysis.…”
Section: Discussionsupporting
confidence: 55%
“…We used the 3RAD protocol as described by Bayona-Vásquez et al 42 to analyze hatchlings from six different nests collected at Abrolhos. Briefly, we digested 100 ng of genomic DNA with the enzymes MseI, EcoRI and CviQI (New England Biolabs) at 10 U/μL and the specific combination of adapters for each sample for 1 h at 37 °C.…”
Section: Methodsmentioning
confidence: 99%
“…Libraries were prepared using the 3RADseq methodology proposed by Bayona‐Vásquez et al . (2019). The technique modifies typical double‐digest restriction site‐associated DNA sequencing (Peterson et al ., 2012) by including a third restriction enzyme that recognizes and cleaves adapter dimer.…”
Section: Methodsmentioning
confidence: 99%
“…Reduced‐representation genotyping approaches constitute a powerful tool for conducting in‐depth population genetics studies for any species of interest. Following the introduction of restriction‐site‐associated DNA sequencing (Baird et al., 2008), a wide range of related methodologies utilizing restriction enzymes have been introduced like genotyping by sequencing (Elshire et al., 2011), ddRAD‐seq (Peterson, Weber, Kay, Fisher, & Hoekstra, 2012), 2b‐RAD (Wang, Meyer, McKay, & Matz, 2012), ezRAD (Toonen et al., 2013), quaddRAD (Franchini, Monné Parera, Kautt, & Meyer, 2017), and 2RAD/3RAD (Bayona‐Vásquez et al., 2019). The aforementioned platforms have been used extensively in studies on aquatic organisms focusing both in population genetic aspects (Andrews, Good, Miller, Luikart, & Hohenlohe, 2016) and in studying traits of interest for farming purposes (Houston et al., 2020; You, Shan, & Shi, 2020).…”
Section: Introductionmentioning
confidence: 99%