Adapting the protocol for studying the functional capacity of T lymphocytes thawed from cryopreservation
E. V. Saidakova,
L. B. Korolevskaya,
V. N. Ponomareva
et al.
Abstract:Background. Immunological studies are impossible without long-term storage of cryopreserved biomaterial. There are no standard procedures for working with cryopreserved mononuclear leukocytes.The aim of the study. To optimize the protocol for culturing T lymphocytes thawed after cryopreservation by assessing their viability and proliferative capacity.Methods. Mononuclear leukocytes were isolated from the peripheral blood of relatively healthy volunteers (n = 18). Cells were subjected to controlled freezing dow… Show more
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