Adaptional evolution of trichome in <i>Caragana korshinskii</i> to natural drought stress on the Loess Plateau, China

Ning, Pengbo; Wang, Junhui; Zhou, Yulu; Gao, Lifang; Wang, Jun; Gong, Chunmei

doi:10.1002/ece3.2157

Cited by 44 publications

(36 citation statements)

References 53 publications

(85 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The annual precipitation, annual evaporation, soil moisture content (SMC) and plant leaf water potential were reported previously [25]. However, no significant difference was detected in the annual average temperature from the northwest to the southeast, soil pH and altitude along the precipitation gradient [26]. The collection and freezing of plant materials and soil samples with respect to C. korshinskii were described previously [27].…”

Section: Sampling Sites Conditions and Plant Materialsmentioning

confidence: 99%

Caragana korshinskiiphenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress

Liu

Xie

Yao

et al. 2019

Biotechnology & Biotechnological Equipment

Self Cite

View full text Add to dashboard Cite

Drought is one of the most severe abiotic stresses, the damage due to which, various plant species mitigate by activating mechanisms that are not yet well understood. Caragana korshinskii is a xerophytic shrub found in the semi-arid regions of northwest China with high tolerance to several abiotic stresses, including drought. Based on the de novo transcriptome data from C. korshinskii leaflets collected along a precipitation gradient on the Loess Plateau (China), most of the differentially expressed genes were explored using trend analysis along the precipitation gradient. Gene ontological analysis showed that "phenylpropanoid biosynthesis process ! secondary metabolite biosynthetic process" terms were the most significant gene ontologies, whereas Kyoto Encyclopedia of Genes and Genomes-based analysis indicated that the biosynthesis of secondary metabolites was a significant metabolic pathway. Real-time polymerase chain reaction and enzyme activity analyses confirmed the increased transcription of the phenylalanine ammonialyase (PAL) gene in C. korshinskii under drought stress in field and laboratory conditions. These results suggested that C. korshinskii adjusts its secondary metabolism to water-deficit environments and activates PAL by drought stress. Therefore, further studies on the obtained data can expand the current understanding of the molecular and genetic mechanisms responsible for the drought endurance in C. korshinskii.

show abstract

Section: Sampling Sites Conditions and Plant Materialsmentioning

confidence: 99%

Caragana korshinskiiphenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress

Liu

Xie

Yao

et al. 2019

Biotechnology & Biotechnological Equipment

Self Cite

View full text Add to dashboard Cite

show abstract

“…Trichomes affect the optical properties of the leaf surface and may protect plants from stress damage and reduced water loss through decreasing the rate of transpiration [34][35][36]. In the current study, we revealed that the density and number of epidermal trichomes on the abaxial side of leaves were higher in the transgenic plants than in the WT plants.…”

Section: Transgenic Plants Heterologously Expressing Rty Have Increasmentioning

confidence: 44%

Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

Yang

Raza³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Strawberry ( Fragaria × ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutation of Arabidopsis thaliana ROOTY ( RTY ) results in increased endogenous auxin levels and roots and shoot growth, but the effects of this gene in strawberry remain unclear. Results Here, we heterologously expressed Arabidopsis rty in strawberry plants and examined the effects of rty expression on the hormonal and physiological properties of the plants. Heterologous expression of rty induced IAA accumulation and increased the production of adventitious roots as well as trichomes on the abaxial leaf surface of the transgenic plants. Furthermore, the transgenic strawberry plants had increased ABA accumulation and stomatal closure. The transgenic strawberry plants exhibited enhanced water use efficiency and a reduced water loss rate. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the untransformed controls, and the transgenic plants were more drought tolerant than the wild-type plants. Our results uncover a transgenic approaches can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions. Conclusions In this study, the rty gene improves hormone-mediated drought tolerance in transgenic strawberry. We demonstrated that the heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. This study provides the basis for future genetic modifications of strawberry to improve drought tolerance.

show abstract

“…The trichome is an important diagnostic character used in plant identification and plant taxonomy, and is involved in deterring herbivore attack and in adaptation to adverse environments [3,4,54]. Unlike A. thaliana , poplar trichomes never undergo branching.…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Survey Reveals Transcriptional Differences Underlying the Contrasting Trichome Phenotypes of Two Sister Desert Poplars

Ma¹,

He²,

Bai³

et al. 2016

Genes

View full text Add to dashboard Cite

Trichomes, which are widely used as an important diagnostic characteristic in plant species delimitation, play important roles in plant defense and adaptation to adverse environments. In this study, we used two sister poplar species, Populus pruinosa and Populus euphratica—which have, respectively, dense and sparse trichomes—to examine the genetic differences associated with these contrasting phenotypes. The results showed that 42 and 45 genes could be identified as candidate genes related to trichomes in P. pruinosa and P. euphratica, respectively; most of these genes possessed high degrees of diversification in their coding sequences, but they were similar in intron/exon structure in the two species. We also found that most of the candidate trichome genes were expressed at higher levels in P. pruinosa, which has dense trichomes, than in P. euphratica, where there are few trichomes. Based on analyses of transcriptional profiles, a total of 195 genes, including many transcription factors, were found to show distinct differences in expression. The results of gene function annotation suggested that the genes identified as having contrasting levels of expression level are mainly associated with trichome elongation, ATPase activity, and hormone transduction. Changes in the expression of these and other related genes with high sequence diversification may have contributed to the contrast in the pattern of trichome phenotypes between the two species.

show abstract

Adaptional evolution of trichome in Caragana korshinskii to natural drought stress on the Loess Plateau, China

Cited by 44 publications

References 53 publications

Caragana korshinskiiphenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress

Caragana korshinskiiphenylalanine ammonialyase is up-regulated in the phenylpropanoid biosynthesis pathway in response to drought stress

Heterologous Expression of Arabidopsis rty Enhances Drought Tolerance in Strawberry (Fragaria × ananassa Duch.)

Genome-Wide Survey Reveals Transcriptional Differences Underlying the Contrasting Trichome Phenotypes of Two Sister Desert Poplars

Contact Info

Product

Resources

About