Adaptive Acid Tolerance Response of<i>Vibrio parahaemolyticus</i>as Affected by Acid Adaptation Conditions, Growth Phase, and Bacterial Strains

Chiang, Meng‐Tsan; Chou, Chien-Ming; Chen, Hsi-Chia; Tseng, Yu‐Ting; Chen, Ming‐Ju

doi:10.1089/fpd.2011.1112

Cited by 8 publications

(3 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In order to confirm that a mild acid preadaptation phase increases V. parahaemolyticus survival under lethal acid conditions, cells were preadapted at pH 5.5 prior to testing survival at pH 4 (4,40,46,47). It was determined that the 30 min preadaptation phase significantly increased survival at 20 min postlethal acidification in both the wild-type (P < 0.001) and the isogenic AcadA strain (P < 0.01) compared with the survival of unadapted cells (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The basic product cadaverine is excreted from the bacterial cell by the lysinecadaverine antiporter CadB (24,27,35,38,40). Mild acid exposure enhances the survival of pathogens under lethal acid conditions through the acid tolerance response, which has been reported in S. enterica Typhimurium, V. cholerae, and in V. parahaemolyticus (4,8,24,40,46,47). We previously found that cells exposed to lethal acid stress survive poorly when grown in 1% NaCl compared with 3% NaCl and that a short preadaptation phase at 3% NaCl rescues these cells and increases survival (43).…”

mentioning

confidence: 92%

See 1 more Smart Citation

High-Salt Preadaptation of Vibrio parahaemolyticus Enhances Survival in Response to Lethal Environmental Stresses

Kalburge

Whitaker

Boyd

2014

Journal of Food Protection

View full text Add to dashboard Cite

Adaptation to changing environmental conditions is an important strategy for survival of foodborne bacterial pathogens. Vibrio parahaemolyticus is a gram-negative seafoodborne enteric pathogen found in the marine environment both free living and associated with oysters. This pathogen is a moderate halophile, with optimal growth at 3% NaCl. Among the several stresses imposed upon enteric bacteria, acid stress is perhaps one of the most important. V. parahaemolyticus has a lysine decarboxylase system responsible for decarboxylation of lysine to the basic product cadaverine, an important acid stress response system in bacteria. Preadaptation to mild acid conditions, i.e., the acid tolerance response, enhances survival under lethal acid conditions. Because of the variety of conditions encountered by V. parahaemolyticus in the marine environment and in oyster postharvest facilities, we examined the nature of the V. parahaemolyticus acid tolerance response under high-salinity conditions. Short preadaptation to a 6% salt concentration increased survival of the wild-type strain but not that of a cadA mutant under lethal acid conditions. However, prolonged exposure to high salinity (16 h) increased survival of both the wild-type and the cadA mutant strains. This phenotype was not dependent on the stress response sigma factor RpoS. Although this preadaptation response is much more pronounced in V. parahaemolyticus, this characteristic is not limited to this species. Both Vibrio cholerae and Vibrio vulnificus also survive better under lethal acid stress conditions when preadapted to high-salinity conditions. High salt both protected the organism against acid stress and increased survival under -20°C cold stress conditions. High-salt adaptation of V. parahaemolyticus strains significantly increases survival under environmental stresses that would otherwise be lethal to these bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 92%

High-Salt Preadaptation of Vibrio parahaemolyticus Enhances Survival in Response to Lethal Environmental Stresses

Kalburge

Whitaker

Boyd

2014

Journal of Food Protection

View full text Add to dashboard Cite

show abstract

“…Among the test strains, V. parahaemolyticus 690, BCRC 13023, and BCRC 13025, involved in foodborne outbreaks in Taiwan, in lateexponential phase exhibited a greater degree of acid tolerance after acid adaptation at pH 5.5 for 90 min (Chiang et al, 2012b). Furthermore, two-dimensional electrophoresis analysis showed that protein expression also differed between acid-adapted and nonadapted strains of V. parahaemolyticus (Chiang et al, 2012a).…”

Section: Introductionmentioning

confidence: 99%

Effect of Acid Adaptation on the Environmental Stress Tolerance of Three Strains ofVibrio parahaemolyticus

Chiang

Chen²,

Wu³

et al. 2014

Foodborne Pathogens and Disease

Self Cite

View full text Add to dashboard Cite

Three strains of Vibrio parahaemolyticus (690, BCRC 13023, and BCRC 13025), involved in foodborne outbreaks in Taiwan, were subjected to acid adaptation at pH 5.5 for 90 min. The effects of acid adaptation on the tolerance of V. parahaemolyticus to various environmental stresses, including heat (47°C), cold (4°C and -20°C), ethanol (8%), high salt (20% NaCl), and hydrogen peroxide (20 ppm) were examined. Results showed that acid adaptation increased the thermal tolerance of the three test strains of V. parahaemolyticus, while it did not affect their cold tolerance. Acid adaptation also increased the ethanol tolerance in V. parahaemolyticus 690 and BCRC 13025, but not in BCRC 13023. Differences in the tolerance to high salts were noted among the three strains after prior acid adaptation. However, these acid-adapted V. parahaemolyticus strains were more susceptible to hydrogen peroxide than their nonadapted controls. These findings demonstrated that acid adaption responses of V. parahaemolyticus varied among strains and types of stress challenge.

show abstract

Response of Foodborne Pathogens to Acid Stress

Amund

et al. 2022

Stress Responses of Foodborne Pathogens

View full text Add to dashboard Cite

Adaptive Acid Tolerance Response ofVibrio parahaemolyticusas Affected by Acid Adaptation Conditions, Growth Phase, and Bacterial Strains

Cited by 8 publications

References 58 publications

High-Salt Preadaptation of Vibrio parahaemolyticus Enhances Survival in Response to Lethal Environmental Stresses

High-Salt Preadaptation of Vibrio parahaemolyticus Enhances Survival in Response to Lethal Environmental Stresses

Effect of Acid Adaptation on the Environmental Stress Tolerance of Three Strains ofVibrio parahaemolyticus

Response of Foodborne Pathogens to Acid Stress

Contact Info

Product

Resources

About