Adaptive and cross-adaptive responses of persistent and non-persistent Listeria monocytogenes strains to disinfectants

Lundén, Janne; Autio, Tiina; Markkula, Annukka; Hellström, Sanna; Korkeala, Hannu

doi:10.1016/s0168-1605(02)00312-4

Cited by 172 publications

(121 citation statements)

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“…Several studies indicate that the most important source of food contamination with L. monocytogenes is via cross-contamination from the equipment and general environment of the processing plants, where some L. monocytogenes strains can persist over extended periods, while others are recovered only sporadically (30,33,37,38,44,47). While several authors attempted to identify specific characteristics, e.g., biofilm formation or sanitizer resistance capabilities, that may be associated with L. monocytogenes persistence in processing plants (1,6,39,49), little attention has been given to the potential contributions of prophages to the ability of L. monocytogenes to establish persistence. In many pathogens, these bacterial viruses are recognized as important contributors to virulence, in the form of bacterial lysogens, or as vectors in horizontal gene transfer; in addition, the presence of prophages may also confer resistance to certain phages (7,8).…”

mentioning

confidence: 99%

Diverse Geno- and Phenotypes of Persistent Listeria monocytogenes Isolates from Fermented Meat Sausage Production Facilities in Portugal

Ferreira

Barbosa

Stasiewicz

et al. 2011

Appl Environ Microbiol

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The persistence of Listeria monocytogenes in food-associated environments represents a key factor in transmission of this pathogen. To identify persistent and transient strains associated with production of fermented meat sausages in northern Portugal, 1,723 L. monocytogenes isolates from raw material and finished products from 11 processors were initially characterized by random amplification of polymorphic DNA (RAPD), PCR-based molecular serotyping, and epidemic clone characterization, as well as cadmium, arsenic, and tetracycline resistance typing. Pulsed-field gel electrophoresis (PFGE) typing of 240 representative isolates provided evidence for persistence of L. monocytogenes for periods of time ranging from 10 to 32 months for all seven processors for which isolates from different production dates were available. Among 50 L. monocytogenes isolates that included one representative for each PFGE pattern obtained from a given sample, 12 isolates showed reduced invasion efficiency in Caco-2 cells, including 8 isolates with premature stop codons in inlA. Among 41 isolates representing sporadic and persistent PFGE types, 22 isolates represented lysogens. Neither strains with reduced invasion nor lysogens were overrepresented among persistent isolates. While the susceptibility of isolates to lysogenic phages also did not correlate with persistence, it appeared to be associated with molecular serotype. Our data show the following.

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confidence: 99%

Diverse Geno- and Phenotypes of Persistent Listeria monocytogenes Isolates from Fermented Meat Sausage Production Facilities in Portugal

Ferreira

Barbosa

Stasiewicz

et al. 2011

Appl Environ Microbiol

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“…monocytogenes a desinfetantes influenciam a sobrevivência da bactéria nas plantas de processamento de alimentos (Aese et al, 2000;Mereghetti et al, 2000). Os sanificantes são amplamente utilizados em instalações de processamento de alimentos, contudo, apesar da realização do processo de higienização regular, a eliminação de L. monocytogenes tem sido cada vez mais difícil (Lundén et al, 2003).…”

Section: Introductionunclassified

“…Assim, o conhecimento da possível adaptação cruzada de L. monocytogenes a desinfetantes seria valioso ao projetar rotação de desinfetantes no processamento de alimentos (Lundén et al, 2003).…”

Section: Introductionunclassified

Adaptação e adaptação cruzada de Listeria monocytogenes aos compostos eugenol e carvacrol

Souza

Tebaldi

Píccoli

2015

Rev. bras. plantas med.

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RESUMO:A evolução de certos microrganismos permite sua rápida adaptação aos ambientes em constante mudança, desenvolvendo assim, tolerância ou resistência ao aumento de determinados estresses. O uso de compostos bioativos provenientes da flora nativa tem sido apontado como uma possível solução para os problemas de controle da resistência e proliferação bacteriana. Este trabalho visou verificar a adaptação e adaptação cruzada de L. monocytogenes, frente aos compostos fenólicos eugenol e carvacrol. A concentração mínima inibitória (CMI) dos compostos fenólicos foi determinada pela técnica de microdiluição em placas de 96 cavidades, em caldo TSB + 0,5% de Tween 80. As concentrações finais (%) obtidas foram: 0,06; 0,12; 0,24; 0,49; 0,98; 1,95; 3,9; 6,25; 12,5; 25; 50. A suspensão bacteriana padronizada foi inoculada nas cavidades das placas, as quais foram incubadas a 37ºC por 24 horas com posterior leitura da absorbância a 620 nm e determinação da CMI. A adaptação das células de L. monocytogenes ao eugenol e carvacrol foi realizada com o cultivo das células em TSB + 0,06% de eugenol ou carvacrol à 37°C por 2 horas. A cultura foi então centrifugada e as células ressuspendidas e padronizadas em TSB. A seguir, realizou-se novamente a técnica de microdiluição em caldo. Os resultados obtidos demonstraram que L. monocytogenes apresentou adaptação e adaptação cruzada frente ao carvacrol e eugenol. A CMI do eugenol e carvacrol foi de 24%. A pré-exposição de L. monocutogenes a concentração sub-letal de 0,06% de carvacrol ou de eugenol aumentou sua resistência. A pré-exposição ao carvacrol promoveu a adaptação de L.monocytogenes a ele aumentando a CMI para 12,5%. Já para o eugenol a CMI passou para 25%. Quando submetidas à concentração sub-letal de eugenol, este promoveu a adaptação das células tanto ao carvacrol quanto ao eugenol, sendo a CMI de 12,5%. Os resultados obtidos demonstraram que L. monocytogenes apresentou adaptação e adaptação cruzada ao carvacrol e eugenol. O presente trabalho sugere estudos futuros ainda mais abrangentes quanto à potencialidade antimicrobiana destes compostos. Palavras-chave: antimicrobianos naturais, compostos bioativos, patógeno alimentarABSTRACT: Adaptation and Cross adaptation of Listeria monocytogenes to eugenol and carvacrol compounds. Some microorganisms have evolved and therefore are able to rapidly adapt themselves to a constantly changing environment, thus developing tolerance or resistance to the increase of some specific stresses. The use of bioactive compounds from the native vegetation has been pointed out as a possible solution to the problems of control of bacterial resistance and proliferation. This work aimed to check the adaptation and cross adaptation of L. monocytogenes, towards the phenolic compounds eugenol and carvacrol. The minimum inhibitory concentration (MIC) of the phenolic compounds was determined by the microdilution technic in plates of 96 cavities, in CALDO TSB + 0,5% of Tween 80. The final concentrations (%) obtained were: 0,06; 0,12; 0,24; 0,49; 0,98; 1,95;...

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“…Such alterations cause morphological and physicochemical changes in the cell surface and change the minimal inhibitory concentrations of BC MIC in L monocytogenes (138,216). The precise nature of changes in cell surface still remains unclear.…”

Section: Quaternary Ammonium Compound Bezalkonium Chloride (Bc) the mentioning

confidence: 99%

“…However, serotype 4b highly represented in clinical isolates is not the most common serotype in food isolates (138). Even thou relation between serotype and virulence has not been established monocytogenes strains and this could be the reason for faster growth of such strains in milk.…”

mentioning

confidence: 98%

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

Rakić-Martinez¹

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ACKNOWLEDGMENTSI would like to thank Dr. Vera Katić for being my mentor, not only through PhD studies, but in my undergraduate years as well. Her objectivity, articulate thinking and dedication to work inspired me to keep moving forward even after long, tiresome days in the lab. I will forever appreciate Dr. Katic's encouragement to pursue personal happiness and her support in important life decisions.I am forever grateful to Dr. Sophia Kathariou for accepting me in her lab and making me feel at home while far from it. Her incredible ability to find the best solutions when things were not looking promising and open completely new horizons along with her creativity and knowledge made science so attractive. Looking at all the great, bright and skilful young people pursuing their dreams under Dr. Kathariou's guidance makes me very proud for being a part of it.My appreciation also goes to Dr. Lepšanović and Dr. Mišić for accepting to be members of the committee. I am thankful to all my lab mates and to Ms. Robin Siletzky for her solid support throughout my work. A special thanks to Dr. Vikrant Dutta for his patience, willingness to help and his much admired teaching skills.Lastly and foremost I would like to thank my family. To my dear parents Ljubo and Zora whose unconditional love, support and presence whenever I needed them have been and will always be my motive to be better. To my big brother Zoran, who has been, and is my role model, but still owes me German lessons. Finally, I want to thank my boys, my beloved husband Sergio and my precious son Gregory, who have been an endless source of energy, support and love. Thanks for being my wingmen guys! ABSTRACTAbility to contaminate ready-to-eat, highly processed, cold-stored food products, relatively high fatality rate and environmental adaptations define .Listeria monocytogenes as a challenging foodborne pathogen of high public health significance. Listeria's adaptations and prolonged persistence in food processing facilities are a key link to contamination of foods.In recent years, bacterial multidrug resistance has been recognized as an emerging health challenge. Exposure to antimicrobials (antibiotics, disinfectants or environmentally ubiquitous heavy metals), may provide selective pressures for resistance with accompanying cross-resistance and increase in environmental fitness.In this work I addressed some of the possible mechanisms of adaptations that may contribute to persistence of L. monocytogenes in the environment as well as to survival and growth in different foods. This includes adaptations to heavy metals such as cadmium, disinfectants such as benzalkonium chloride (BC), selected antibiotics (e.g. the fluoroquinolone ciprofloxacin) and other toxic compounds (e.g. tetraphenyl-phosponium, often used as an anti-carcinogenic and efflux pump substrate). In order to characterize the impact of multidrug resistance on survival and growth of L. monocytogenes in different foods, we tested mutants adapted to higher concentrations of antibiotic ciprofloxacin and ...

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Adaptive and cross-adaptive responses of persistent and non-persistent Listeria monocytogenes strains to disinfectants

Cited by 172 publications

References 12 publications

Diverse Geno- and Phenotypes of Persistent Listeria monocytogenes Isolates from Fermented Meat Sausage Production Facilities in Portugal

Diverse Geno- and Phenotypes of Persistent Listeria monocytogenes Isolates from Fermented Meat Sausage Production Facilities in Portugal

Adaptação e adaptação cruzada de Listeria monocytogenes aos compostos eugenol e carvacrol

Identification of the mechanisms of resistance and potential cross resistance of Listeria monocytogenes upon adaptation to different antimicrobials

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