Adaptive capability as indicated by behavioral and physiological responses, plasma HSP70 level, and PBMC HSP70 mRNA expression in Osmanabadi goats subjected to combined (heat and nutritional) stressors

Shilja, Shaji; Sejian, Veerasamy; Bagath, M.; Mech, A.; David, C. G.; Kurien, E. K.; Varma, Girish; Bhatta, Raghavendra

doi:10.1007/s00484-015-1124-5

Cited by 103 publications

(81 citation statements)

References 33 publications

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“…Our HS pregnant goats experienced greater RT (+0.68˚C) and RR (+76 breaths/min), but lower feed intake by 15% compared to TN goats. These findings indicate that HS treatment effectively triggered a significant heat stress response during pregnancy, which agrees with previous results obtained in goats exposed to HS conditions [24][25][26]. We hypothesized that heat stress during early gestation would influence both gestation and the development of the offspring postnatally.…”

Section: Discussionsupporting

confidence: 91%

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Prenatal heat stress effects on gestation and postnatal behavior in kid goats

Coloma-García¹,

Mehaba²,

Llonch³

et al. 2020

PLoS ONE

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Consequences of heat stress during pregnancy can affect the normal development of the offspring. In the present experiment, 30 Murciano-Granadina dairy goats (41.8 ± 5.7 kg) were exposed to 2 thermal environments varying in temperature-humidity index (THI) from 12 days before mating to 45 days of gestation. The environmental conditions were: gestation under thermal-neutral (TN; THI = 71 ± 3); and gestation under heat stress (HS; THI = 85± 3) conditions. At 27 ± 4 days old, female kids exposed to in utero TN (IUTN; n = 16) or in utero HS (IUHS; n = 10) were subjected to 2 tests: arena test (AT) and novel object test (NOT), the latter was repeated at 3 months of age. Additionally, 8 months after birth, a subset of IUTH and IUHS growing goats (n = 8 each; 16.8 ± 3.4 kg BW) were exposed to 2 environmental conditions in 2 consecutive periods: a basal thermal-neutral period (THI = 72 ± 3) for 7 days, and a heat-stress period (THI = 87 ± 2) for 21 days. In both periods, feeding, resting, posture, and thermally-associated behaviors were recorded. The gestation length was shortened by 3 days in GHS goats. In the AT, IUHS kids showed a lower number of sniffs (P < 0.01) compared to IUTN. In the NOT, IUHS kids also tended to show a lower number of sniffs (P = 0.09). During heat exposure, IUTN and IUHS growing goats spent more time resting and exhibited more heat-stress related behaviors such as panting and drinking (P < 0.001); however, no differences were observed between both groups. In conclusion, heat stress during the first third of pregnancy shortened gestation length and influenced the exploratory behavior of the kids in the early life. However, behavior responses to heat stress during the adulthood were not affected by the in utero thermal treatment.

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Section: Discussionsupporting

confidence: 91%

“…Resting and drinking increased dramatically during the first week of heat exposure. Lying and drinking behaviors could be considered as ideal biological markers for assessing the severity of the heat stress response [26]. Similarly, exploratory, grooming, and feeding behaviors declined throughout the entire period of heat exposure.…”

Section: Discussionmentioning

confidence: 99%

Prenatal heat stress effects on gestation and postnatal behavior in kid goats

Coloma-García¹,

Mehaba²,

Llonch³

et al. 2020

PLoS ONE

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“…The relative rumen HSP70 mRNA expression was carried out as per the protocol of Shilja et al (2015). Table 3 describes the primer sequences for HSP70 and the reference gene Glyceraldehyde 3-phosphate dehydrogenase (GAPDH).…”

Section: Rumen Hsp70 Gene Expressionmentioning

confidence: 99%

Impact of heat stress, nutritional stress and combined (heat and nutritional) stresses on rumen associated fermentation characteristics, histopathology and HSP70 gene expression in goats

Chaidanya

Soren

Sejian

et al. 2017

JABB

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A study was conducted to assess the impact of heat stress, nutritional stress and combined stresses (heat and nutritional stress) on rumen fermentation characteristics, histopathology of rumen and rumen HSP70 gene expression in goats. Twenty four adult Osmanabadi bucks were divided into four groups, C (n=6; control), HS (n=6; heat stress), NS (n=6; nutritional stress) and CS (n=6; combined stress). The study was conducted for a period of 45 days. The C and HS bucks had ad libitum access to their feed while NS and CS bucks were subjected to restricted feed (30% intake of C bucks) to induce nutritional stress. The HS and CS bucks were exposed to heat stress in an outside environment. Both feed intake and body weight were significantly (p < 0.01) lower in CS and NS groups. The carboxy methyl cellulase activities-extracellular, intracellular and total activity in the rumen fluid differed significantly (p < 0.01) between the groups. The highest concentration of ammonia nitrogen (p < 0.05) was recorded in C while the lowest in the CS group. The concentration of total nitrogen and trichloroacetic acid precipitable N, propionic acid, butyric acid, and valeric acid was lower (p < 0.01) in the restricted fed (NS and CS) bucks as compared to ad libitum fed groups (C and HS). Further, the ratio of acetate to propionate (A:P ratio) was also significantly (p < 0.01) higher in CS and NS groups. The higher expression of rumen heat shock protein 70 (HSP70) mRNA was observed in CS goats. The histopathological section of rumen revealed a reduction in the length of rumen villi and thickness in CS, whereas rumen keratinization was highest in the CS group. From the study it can be concluded that when two stresses occur simultaneously, they may have severe impact on rumen fermentation characteristics of bucks.

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“…Dietary energy plays important role in nutrient utilization and thereby affects adaptive capability of goats [10,11]. Animals fed on different energy levels in the diet significantly affect the total feed intake, metabolizable energy and the average daily gain, and thereby affect their adaptive capability [12,13].…”

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confidence: 99%

Effect of Different Diet Level on the Physiological Adaptability, Biochemical and Endocrine Responses and Relative Hepatic HSP70 and HSP90 Genes Expression in Osmanabadi Kids

Sejian¹,

Bagath²,

Parthipan³

et al. 2015

JAST-A

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Abstract:The study was conducted to investigate the impact of different levels of feed on the adaptive capability based on physiological, blood biochemical, endocrine and molecular mechanisms in growing Osmanabadi kids. The primary objective of the study was to identify if HSP70 and HSP90 can be a nutritional stress marker for goat. The study was conducted for a period of two months. The animals were randomly divided into three groups as GI (n = 6; ad libitum feeding), GII (n = 6; 20% less than ad libitum) and GIII (n = 6; 40% less than ad libitum). The animals were fed with feed consisting of 50% roughage and 50% concentrate. Blood collection was carried out at fortnightly intervals. Body weights were recorded at weekly interval. Physiological responses, biochemical responses, plasma tri-iodo-thyronine (T 3 ), thyroxin (T 4 ) and cortisol were recorded at fortnightly interval. At the end of study period, only GI and GIII animals were slaughtered and different organs were collected for histopathological studies as well as for hepatic HSP70 and HSP90 mRNA transcript expression. Body weight recorded showed significant (P < 0.01) differences between the groups. Physiological responses showed significant (P < 0.01) variation among the groups. Among the biochemical parameters, plasma glucose and total plasma protein and globulin showed significant (P < 0.01) differences between the groups. Plasma T3 (P < 0.01), T4 (P < 0.01) and cortisol (P < 0.05) also differed significantly between the groups. The relative hepatic HSP70 mRNA transcript expression was significantly (P < 0.05) higher in GIII (2.8 fold) as compared to GI (1 fold) kids. Similar result was obtained for hepatic HSP90 mRNA transcript expression. From the results, it can be concluded that Osmanabadi kids possessed the ability to alter their adaptive mechanisms to maintain homeostasis. Further, the study revealed the significance of providing the optimum nutrition for these animals to adapt to existing environmental conditions. The study also established that respiration rate (RR), rectal temperature (RT), T 3 , T 4 and cortisol are considered as nutritional stress markers for goat. Further, the results revealed that probably this is the first study to establish the nutritional stress impact on heat shock protein (HSP) expression in goats. The study identified both HSP70 and HSP90 to be the ideal molecular markers for feed deficit in goats.

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Adaptive capability as indicated by behavioral and physiological responses, plasma HSP70 level, and PBMC HSP70 mRNA expression in Osmanabadi goats subjected to combined (heat and nutritional) stressors

Cited by 103 publications

References 33 publications

Prenatal heat stress effects on gestation and postnatal behavior in kid goats

Prenatal heat stress effects on gestation and postnatal behavior in kid goats

Impact of heat stress, nutritional stress and combined (heat and nutritional) stresses on rumen associated fermentation characteristics, histopathology and HSP70 gene expression in goats

Effect of Different Diet Level on the Physiological Adaptability, Biochemical and Endocrine Responses and Relative Hepatic HSP70 and HSP90 Genes Expression in Osmanabadi Kids

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