Adaptive changes in photosynthetic performance and secondary metabolites during white dead nettle micropropagation

Kapchina-Toteva, Veneta; Dimitrova, Mariya; Stefanova, M.; Koleva, Dimitrina; Kostov, Kaloyan; Yordanova, Zhenya; Stefanov, D.; Zhiponova, Miroslava

doi:10.1016/j.jplph.2014.05.010

Cited by 26 publications

(20 citation statements)

References 31 publications

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“…The data collected in this research showed that cultures grown continuously with no transfers to fresh medium and absence of shoot cutting resulted in shoots >40% larger than those sequentially severed, separated and transferred to fresh medium periodically during the 120 days of the research; this may indicate that subculture induces some stress levels by structure severing and high nutrient saturation on fresh medium that negatively affect shoot growth . Plant size is especially important for plant survival during transfer to ex vitro conditions; in vitro propagated plants grow under very low light intensities (<60 µmol m -2 s -1 ) that prevents photosynthesis activation and therefore, energetic compound supply, usually sucrose, in needed, creating a heterotrophic (photomixotrophic) model for in vitro plant growth and development (Kapchina-Toteva et al, 2014;Nunes et al, 2016;Chavan et al, 2014). Because of this external energetic support, when transferred to ex vitro conditions, plants must have plenty of carbohydrate reserves in organs, such as leaves and stems, that serve as "life boat" effect before newly photosynthetic active leaves are formed.…”

Section: Resultsmentioning

confidence: 99%

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Lopez

Suarez

2018

rcia

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Arrow cane (Gynerium sagitatum Aubl.) is a Poaceae species used as fiber source to make traditional and valuable handmade craftsmanship by indigenous communities in Northern Colombia. Since no commercial crops are established fiber needs are taken from natural plant populations affecting ecosystem. A micropropagation protocol to clonally multiply large quantities of arrow cane plant material for planting commercial crops has been developed; however, micropropagated plants are costly compared to naturally extracted plant material. To reduce micropropagated plants costs, in the present research a double phase medium formulation along with continuous shoot culture with no periodic transfers to fresh medium was compared to semisolid medium system with subculture every four weeks with respect to multiplication rate and costs of micropropagated plants. The results showed that continuous culture of explants with double phase medium and no periodic transfers resulted in higher multiplication rates and larger shoots compared to shoots cultured using the conventionalsemisolid medium system and transfer to fresh medium every four weeks. Plants from both, semisolid and double phase culture system, fully adapted and recovered when transferred to ex vitro conditions. The cost analysis showed that double phase cultured shoots are ≥20% less expensive.

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Section: Resultsmentioning

confidence: 99%

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Lopez

Suarez

2018

rcia

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“…The in vitro environment affected the organization of chloroplasts of A. bromeliifolia, different from other organelles that were similar to those of plants gown in the greenhouse, as reported by Rodrigues et al (2014). The most frequent changes in the ultrastructure of leaf cells of plants cultivated in vitro are observed in the chloroplasts, which vary in shape, size, organization of the internal membrane system and number of starch grains (Wetzstein & Sommer 1982;Lee et al 1985;Majada et al 2002;Sáez et al 2012b;Kapchina-Toteva et al 2014;Stefanova et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Influence of test tube sealing on the morpho-anatomy and ultrastructure of leaves of Aechmea bromeliifolia (Bromeliaceae) grown in vitro

et al. 2020

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In vitro grown plants may have abnormal structural and physiological features. However, the type of the sealing material used in tissue culture may minimize such abnormalities. This study evaluates the influence of the type of sealing of test tubes on the anatomical and ultrastructural features of leaves of Aechemea bromeliifolia (Rudge) Baker (Bromeliaceae), an ornamental bromeliad native to Brazil, grown in vitro. Three types of sealing were used: rigid polypropylene cap (PC), polyvinyl chloride film (PVC), and PC coupled with a microporous membrane (PM). Seedlings germinated in a greenhouse were also studied for comparison. Plants grown in test tubes sealed with PM were more similar to those from the greenhouse, as far as the pattern of stomatal opening, the presence of starch grains, and the organization of the internal membrane system of the chloroplasts is concerned. Plants cultivated in test tubes sealed with PC had higher stomatal density and the chloroplasts had large areas without thylakoids in the stroma. Plants grown in test tubes sealed with PVC had few or no starch grains. These results suggest that microporous membrane used coupled with PC sealing provided natural ventilation, thus contributing to a better plant development.

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“…Для вирішення проблеми підвищення вмісту ефірних олій і створення нових, високопродуктивних сортів в Україні найефективнішим уявляється тестування зразків дикої флори, використання їх як вихідного матеріалу для селекційної роботи та швидке мікроклональне розмноження елітних зразків (Kudrjashova, 2009;Kapchina-Toteva et al, 2014). Метод мікроклонального розмноження стає все розповсюдженішим у світі через свою здатність швидко збільшувати кількість генетично ідентичних і оздоровлених саджанців (Yildirim, 2013;Dakah et al, 2014;Ozdemir et al, 2014).…”

Section: вступunclassified

Optimization of microclonal propagation in vitro of oregano (Origanum vulgare)

et al. 2018

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Medicinal plants are important objects for botany, systematics and plant geography research, as well as physiology, pharmacology, and biotechnology. Medicinal plants from the Lamiaceae family are being intensively studied for medical and pharmacological reasons. This family also includes the medicinal herbaceous plant oregano (Origanum vulgare L.), known from ancient times for its antimicrobial and antifungal properties, the ability to strengthen the human immune system, and to improve the general state of an organism. At present, the study of its antimicrobial, antifungal, insecticidal, anticoagulant, antitumor, therapeutic and many other properties is being actively continued. Due to the relevance of the development of the principles of O. vulgare micropropagation in vitro and the undeveloped conditions and methods of its cultivation, the aim of this work was to optimize microclonation in vitro of oregano via the activation of auxiliary buds. The research tasks were to test the ability of auxiliary buds to be activated depending on the localization on the donor shoot internodes and to intensify the root formation of cuttings through medium content optimization. The influence of the location of the auxiliary buds on donor shoots on their activation in vitro was studied on such indicators as the length of newly formed shoots, the number of nodules per one newly formed shoot, and the number of newly formed shoots per one bud. In plant microclonal propagation, the stage of root formation is very important for further adaptation in soil. Practical experience has shown that for the effective adaptation of oregano in soil, the length of the root system for cuttings should be 1.5–2.0 cm, the degree of root system development – 4–5 points under shoot length of 3–5 cm. The study of peculiarities of oregano microclonal propagation via activation of auxiliary buds has allowed us to optimize the stage of explant selection for cutting and the formation of cuttings’ roots. It has been established that for optimal length, the number of nodules of the newly formed shoots and the number of newly formed shoots, the first internode, located on the top of a parent shoot, as well as the third to fifth ones are more suitable. For rooting oregano cuttings, the optimal medium on the ratio of length and density of root system and on shoot length is the nutrient one containing half of the macro-, microsalts and vitamins on Murashige-Skoog, 20 g/l sucrose and 0.75 mg/l kinetin.

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Adaptive changes in photosynthetic performance and secondary metabolites during white dead nettle micropropagation

Cited by 26 publications

References 31 publications

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Influence of test tube sealing on the morpho-anatomy and ultrastructure of leaves of Aechmea bromeliifolia (Bromeliaceae) grown in vitro

Optimization of microclonal propagation in vitro of oregano (Origanum vulgare)

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