Large-eddy simulation (LES) and Reynolds-Averaged Navier–Stokes (RANS) equations were used to study incompressible flow and heat transfer in a U-duct with a high-aspect-ratio trapezoidal cross section. For the LES, the WALE subgrid-scale model was employed, and its inflow boundary condition was provided by a concurrent LES of incompressible fully-developed flow in a straight duct with the same cross section and flow conditions as the U-duct. LES results are presented for turbulent kinetic energy, Reynolds stresses, pressure–strain rate, turbulent diffusion, turbulent transport, and velocity–temperature correlations, with a focus on how they are affected by the U-turn region of the U-duct. The LES results were also used to assess three commonly used RANS models: the realizable k-ε with the two-layer model in the near-wall region, the two-equation shear-stress transport model, and the seven-equation stress-omega Reynolds stress model. Results obtained show steady and unsteady RANS to incorrectly predict the effects of unsteady flow separation. The results obtained also identified the terms in the RANS models that need to be modified and suggested how turbulent diffusion should be modeled when there is unsteady flow separation.