2016
DOI: 10.1016/j.biortech.2016.03.093
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Adaptive evolution of Schizochytrium sp. by continuous high oxygen stimulations to enhance docosahexaenoic acid synthesis

Abstract: Adaptive laboratory evolution (ALE) is an effective method in changing the strain characteristics. Here, ALE with high oxygen as a selection pressure was applied to improve the production capacity of Schizochytrium sp. Results showed that cell dry weight (CDW) of endpoint strain was 32.4% higher than that of starting strain. But slight lipid accumulation impairment was observed. These major performance changes were accompanied with enhanced isocitrate dehydrogenase enzyme activity and reduced ATP:citrate lyase… Show more

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Cited by 90 publications
(45 citation statements)
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“…As is well known, oxygen supply is a crucial factor for cell growth and lipid accumulation in Schizochytrium sp. In recent years, we focused on investigating the influence of oxygen on DHA production and developed diverse oxygen control strategies to increase DHA production [ 27 29 ]. However, the specific expression of genes involved in the cell response to oxygen has not yet been explored at the transcriptional level for Schizochytrium sp.…”
Section: Discussionmentioning
confidence: 99%
“…As is well known, oxygen supply is a crucial factor for cell growth and lipid accumulation in Schizochytrium sp. In recent years, we focused on investigating the influence of oxygen on DHA production and developed diverse oxygen control strategies to increase DHA production [ 27 29 ]. However, the specific expression of genes involved in the cell response to oxygen has not yet been explored at the transcriptional level for Schizochytrium sp.…”
Section: Discussionmentioning
confidence: 99%
“… 10% and 20% CO 2 97 days Enhanced CO 2 fixation capability and carotenoids accumulation [ 123 ] Schizochytrium sp. Agitation at 230 rpm 40 days Maximum cell dry weight and DHA yield were observed [ 131 ] …”
Section: Adaptive Laboratory Evolutionmentioning
confidence: 99%
“…Many studies have been conducted to optimize the media composition and culture conditions, including nutrient deprivation, strain adaption, temperature shift and genetic engineering to enhance the lipid accumulation efficiency in Schizochytrium sp. (Sakaguchi et al, 2012;Ren et al, 2014Ren et al, , 2015Sun et al, 2014Sun et al, , 2016. However, Schizochytrium sp.…”
Section: Introductionmentioning
confidence: 99%