Adaptive in vivo device for theranostics of inflammation: Real-time monitoring of interferon-γ and aspirin

Cao, Chaomin; Jin, Ronghua; Wei, Hui; Liu, Zhongning; Ni, Shengnan; Liu, Guojun; Young, Howard A.; Chen, Xin; Liu, Guozhen

doi:10.1016/j.actbio.2019.10.021

Cited by 22 publications

(29 citation statements)

References 39 publications

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“…[ 26 ] A molecular beacon aptamer based biosensing device has been developed toward the near real‐time [ 36 ] and real‐time monitoring of IFN‐ γ . [ 121 ] Such a platform has been proven sensitive for the detection of cytokines in the pg mL −1 range. Specifically, we developed a proof‐of‐concept in vivo sensing device for simultaneously monitoring IFN‐ γ at a sensitivity of 10 pg mL −1 and the subsequent release of aspirin triggered by IFN‐ γ .…”

Section: Quantification Of Cytokinesmentioning

confidence: 99%

Cytokines: From Clinical Significance to Quantification

et al. 2021

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Cytokines are critical mediators that oversee and regulate immune and inflammatory responses via complex networks and serve as biomarkers for many diseases. Quantification of cytokines has significant value in both clinical medicine and biology as the levels provide insights into physiological and pathological processes and can be used to aid diagnosis and treatment. Cytokines and their clinical significance are introduced from the perspective of their pro‐ and anti‐inflammatory effects. Factors affecting cytokines quantification in biological fluids, native levels in different body fluids, sample processing and storage conditions, sensitivity to freeze‐thaw, and soluble cytokine receptors are discussed. In addition, recent advances in in vitro and in vivo assays, biosensors based on different signal outputs and intracellular to extracellular protein expression are summarized. Various quantification platforms for high‐sensitivity and reliable measurement of cytokines in different scenarios are discussed, and commercially available cytokine assays are compared. A discussion of challenges in the development and advancement of technologies for cytokine quantification that aim to achieve real‐time multiplex cytokine analysis for point‐of‐care situations applicable for both biomedical research and clinical practice are discussed.

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Section: Quantification Of Cytokinesmentioning

confidence: 99%

Cytokines: From Clinical Significance to Quantification

et al. 2021

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“…Electrochemical (EC) biosensors are principled on the transformation of biochemical information which would be the cytokine concentrations into an analytically interpretable signal, such as current or voltage [ 55 ]. We identify five studies that were based on electrochemical detection: (1) a biosensor based on aptamer technology to release a drug upon the detection of target cytokine [ 31 ]; (2) a multiplexed electrochemical microfluidic immunoarray with eight 32-sensor electrochemical arrays connected with a miniaturized 8-port manifold [ 32 ]; (3) an immunoassay with a sequentially multiplexed amperometry on a single-chip potentiostat [ 33 ]; (4) a testing platform built on Proxim Technology (San Jose, CA, USA) [ 34 ]; and (5) a hybrid magneto-electrochemical miniaturized sensor [ 35 ].…”

Section: Resultsmentioning

confidence: 99%

“…All previously listed immunoassays delivered measures in vitro. One immunoassay in this family that could deliver measurements in vivo was the aptamer-based biosensor [ 31 ] (cf. 1st row in Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…We categorize the methods of the retained papers on cytokine detection into three different categories [ 25 ] based on the signal detection approach they utilize: (1) Optical signal detection [ 26 , 27 , 28 , 29 , 30 ], (2) Electrochemical signal detection [ 31 , 32 , 33 , 34 , 35 ], and (3) Colorimetric signal detection [ 5 , 36 , 37 ], LFA (Milenia Biotec) and LFA (Antagen hIL-8 XpressCard).…”

Section: Methodsmentioning

confidence: 99%

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Potential of Point-of-Care and At-Home Assessment of Immune Status via Rapid Cytokine Detection and Questionnaire-Based Anamnesis

Jamaludeen

Beyer

Billing

et al. 2021

Sensors

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Monitoring the immune system’s status has emerged as an urgent demand in critical health conditions. The circulating cytokine levels in the blood reflect a thorough insight into the immune system status. Indeed, measuring one cytokine may deliver more information equivalent to detecting multiple diseases at a time. However, if the reported cytokine levels are interpreted with considering lifestyle and any comorbid health conditions for the individual, this will promote a more precise assessment of the immune status. Therefore, this study addresses the most recent advanced assays that deliver rapid, accurate measuring of the cytokine levels in human blood, focusing on add-on potentials for point-of-care (PoC) or personal at-home usage, and investigates existing health questionnaires as supportive assessment tools that collect all necessary information for the concrete analysis of the measured cytokine levels. We introduced a ten-dimensional featuring of cytokine measurement assays. We found 15 rapid cytokine assays with assay time less than 1 h; some could operate on unprocessed blood samples, while others are mature commercial products available in the market. In addition, we retrieved several health questionnaires that addressed various health conditions such as chronic diseases and psychological issues. Then, we present a machine learning-based solution to determine what makes the immune system fit. To this end, we discuss how to employ topic modeling for deriving the definition of immune fitness automatically from literature. Finally, we propose a prototype model to assess the fitness of the immune system through leveraging the derived definition of the immune fitness, the cytokine measurements delivered by a rapid PoC immunoassay, and the complementary information collected by the health questionnaire about other health factors. In conclusion, we discovered various advanced rapid cytokine detection technologies that are promising candidates for point-of-care or at-home usage; if paired with a health status questionnaire, the assessment of the immune system status becomes solid and we demonstrated potentials for promoting the assessment tool with data mining techniques.

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“…In support of this notion, we have previously observed similar pattern of inflammatory cytokines response in cortex region with a protection against depression in the BXD21/TyJ RI strain without oxidative stress response 20 . In order to maintain normal homeostasis complex interactions occur between cytokines, inflammation, and the adaptive and innate responses 34 . In the BXD21/TyJ RI strain, the innate activation of cytokines observed herein concomitant with the short immobility time and thus reduced depression-like behavior 20 provides additional impetus for studying novel antidepressants in a BXD RI animal model of innate inflammation.…”

Section: Discussionmentioning

confidence: 99%

The BXD21/TyJ recombinant inbred strain as a model for innate inflammatory response in distinct brain regions

López-Granero

Ferrer

Santos

et al. 2020

Sci Rep

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Oxidative stress and inflammatory cytokines affect the human brain, increasing the risk for mood and cognitive disorders. Such risk might be selective to brain-specific regions. Here, we determined whether BXD recombinant inbred (RI) mice strains are more suitable than C57BL/6J mice for the understanding of the relationship between antioxidant response and inflammatory responses. We hypothesized that inflammatory responses could be independent of antioxidant response and be inherent to brain-specific regions. This hypothesis will be addressed by the analyses of mRNA expression. We explored, at 7-months-of-age, the innate activation of proinflammatory cytokines (tumor necrosis factor alpha (TNFα) and interleukin 6 (IL-6), as well as Kelch-like ECH-associating protein 1 (Keap1), nuclear factor erythroid 2 related factor 2 (Nrf2) and glutathione peroxidase 1 (Gpx1) mRNA in both male and female BXD84/RwwJ RI, BXD21/TyJ RI and control strain (C57BL/6J mice). We report that: (1) The cerebellum is more sensitive to antioxidant response in the BXD21/TyJ RI strain; (2) The cerebellum, hippocampus and striatum show increased levels of cytokines in the BXD21/TyJ RI strain; (3) The BXD RI strain has lower brain weight relative to control strain (C57BL/6 mice). In conclusion, our novel data show the utility of the BXD21/TyJ RI strain mice in offering mechanistic insight into Nrf2's role in the inflammatory system. Central nervous system (CNS) dysfunction is frequently accompanied by oxidative stress and inflammatory responses 1. Oxidative damage and inflammatory cytokines influence brain function and result in increased risk for mood, behavioral and cognitive disorders 2-4. Thus, the general hypothesis is that antioxidant defenses and inflammatory cytokines are key elements in CNS pathologies 5 and psychiatric disorders 3,4,6,7. Several studies have revealed the mechanism by which continued oxidative stress can lead to chronic inflammation, which, in turn, could mediate most chronic diseases 8. The disruption of the inflammatory and oxidative stress pathways is associated with multiple neurotoxic exposures 9-11. Oxidative stress acts activating a variety of transcription factors, including Nrf2. Upon oxidative stress generation, Nrf2 dissociates from Kelch-like ECH-associating protein 1 (Keap1), and translocates into the nucleus where it binds to the antioxidant response element (ARE), and initiates antioxidant gene transcription thus restoring cellular redox homeostasis 12-15. Activation of these transcription factors can lead to the expression of different genes, including those for inflammatory cytokines and anti-inflammatory molecules 8. Indeed, Nrf2 is essential for protection against oxidative stress and it has also been shown to attenuate inflammation 16. Consistent with these observations, published data have corroborated dysregulation of inflammatory and oxidative systems both in behavioral and psychiatric disorders as a consequence of altered Nrf2 pathway functioning 7,15. Neuroinflammatory processes are established...

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Adaptive in vivo device for theranostics of inflammation: Real-time monitoring of interferon-γ and aspirin

Cited by 22 publications

References 39 publications

Cytokines: From Clinical Significance to Quantification

Cytokines: From Clinical Significance to Quantification

Potential of Point-of-Care and At-Home Assessment of Immune Status via Rapid Cytokine Detection and Questionnaire-Based Anamnesis

The BXD21/TyJ recombinant inbred strain as a model for innate inflammatory response in distinct brain regions

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