2021
DOI: 10.3389/fmicb.2021.737248
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Adaptive Laboratory Evolution of Microalgae: A Review of the Regulation of Growth, Stress Resistance, Metabolic Processes, and Biodegradation of Pollutants

Abstract: Adaptive laboratory evolution (ALE) experiments are a serviceable method for the industrial utilization of the microalgae, which can improve the phenotype, performance, and stability of microalgae to obtain strains containing beneficial mutations. In this article, we reviewed the research into the microalgae ALE test and assessed the improvement of microalgae growth, tolerance, metabolism, and substrate utilization by ALE. In addition, the principles of ALE and the key factors of experimental design, as well a… Show more

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Cited by 20 publications
(15 citation statements)
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References 78 publications
(141 reference statements)
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“…Furthermore, it also avoids the introduction of foreign genetic material into the target cells; thus, the improved strains pose no regulatory issues due to the long biosafety record of such technologies ( Figure 1 B) [ 48 , 49 ]. In adaptive laboratory evolution experiments, cell cultures are subjected to a continuous selective pressure over a long period of time [ 25 , 40 , 41 , 50 ]. Consequently, selection can act on all traits responsible for a suitable improvement under the environmental regimes of interest [ 25 , 40 , 41 , 49 , 51 ].…”
Section: Strategies For Microalgal Strain Improvementmentioning
confidence: 99%
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“…Furthermore, it also avoids the introduction of foreign genetic material into the target cells; thus, the improved strains pose no regulatory issues due to the long biosafety record of such technologies ( Figure 1 B) [ 48 , 49 ]. In adaptive laboratory evolution experiments, cell cultures are subjected to a continuous selective pressure over a long period of time [ 25 , 40 , 41 , 50 ]. Consequently, selection can act on all traits responsible for a suitable improvement under the environmental regimes of interest [ 25 , 40 , 41 , 49 , 51 ].…”
Section: Strategies For Microalgal Strain Improvementmentioning
confidence: 99%
“…In adaptive laboratory evolution experiments, cell cultures are subjected to a continuous selective pressure over a long period of time [ 25 , 40 , 41 , 50 ]. Consequently, selection can act on all traits responsible for a suitable improvement under the environmental regimes of interest [ 25 , 40 , 41 , 49 , 51 ]. Thus, spontaneous, adaptive and non-specific mutations are introduced into the genome and passed down from mother to daughter cells [ 41 , 51 , 52 ].…”
Section: Strategies For Microalgal Strain Improvementmentioning
confidence: 99%
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“…However, other parts have not been investigated at all. To approach the signals on the C-terminal extension, I think that application of protein-engineering technology, such as iterative saturation mutagenesis and adaptive laboratory evolution, is a good option [ 158 , 159 , 160 , 161 ]. After laboratory evolution under appropriate design and conditions, if sequence convergence can be detected on the C-terminal extension, which proves that the C-terminal extension carries some signals supplying benefit for surviving these conditions.…”
Section: Remaining Mysteries In the D1 Processingmentioning
confidence: 99%
“…High-light- and high-temperature-tolerances are especially important for outdoor applications. Adaptive laboratory evolution (ALE) in combination with systems biology approaches, were successfully applied for Chlamydomonas, Synechocystis , and Synechococcus ( Zhang et al, 2021b ; Srivastava et al, 2021 ; LaPanse et al, 2021 ; Dann et al, 2021 ; Mukherjee et al, 2020 ). Synechocystis sp.…”
Section: Pathway Engineeringmentioning
confidence: 99%