Adaptive optics for enhanced signal in CARS microscopy

Wright, Amanda J.; Poland, Simon P.; Girkin, John M.; Freudiger, Christian W.; Evans, Conor L.; Xie, X. Sunney

doi:10.1364/oe.15.018209

Cited by 97 publications

(64 citation statements)

References 29 publications

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“…Such adaptive optics method may not work when transmission signals acquired from reference and scattered beams lack sufficient correlation. Adaptive optics approaches have also been applied to CARS microscopy, by using the maximization of the CARS intensity as an objective function to optimize the shaping of the excitation wavefront [12].…”

Section: Introductionmentioning

confidence: 99%

“…Virtually all adaptive optics approaches are based on empirical optimization of experimentally accessible parameters, such as the signal intensity [8][9][10][11][12]. In this approach, the sample is considered a black box, which can be characterized by an effective transmission matrix that does not require a detailed understanding of the physical origin of the wavefront distortions.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of scattering on coherent anti-Stokes Raman scattering (CARS) signals

Ranasinghesagara¹,

Vito²,

Piazza³

et al. 2017

Opt. Express

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Abstract:We develop a computational framework to examine the factors responsible for scattering-induced distortions of coherent anti-Stokes Raman scattering (CARS) signals in turbid samples. We apply the Huygens-Fresnel Wave-based Electric Field Superposition (HF-WEFS) method combined with the radiating dipole approximation to compute the effects of scatteringinduced distortions of focal excitation fields on the far-field CARS signal. We analyze the effect of spherical scatterers, placed in the vicinity of the focal volume, on the CARS signal emitted by different objects (2µm diameter solid sphere, 2µm diameter myelin cylinder and 2µm diameter myelin tube). We find that distortions in the CARS signals arise not only from attenuation of the focal field but also from scattering-induced changes in the spatial phase that modifies the angular distribution of the CARS emission. Our simulations further show that CARS signal attenuation can be minimized by using a high numerical aperture condenser. Moreover, unlike the CARS intensity image, CARS images formed by taking the ratio of CARS signals obtained using xand y-polarized input fields is relatively insensitive to the effects of spherical scatterers. Our computational framework provide a mechanistic approach to characterizing scattering-induced distortions in coherent imaging of turbid media and may inspire bottom-up approaches for adaptive optical methods for image correction.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of scattering on coherent anti-Stokes Raman scattering (CARS) signals

Ranasinghesagara¹,

Vito²,

Piazza³

et al. 2017

Opt. Express

View full text Add to dashboard Cite

show abstract

“…For this and other reasons, sensorless AO techniques (that is AO setups which do not use a WS) have been developed, obtaining extremely good results, usually at the expenses of only a reduced correction speed. Among the others, the sensorless AO techniques which makes use of optimization algorithms have been very successful: for example, since their first use (Judson & Rabitz 1992) random search algorithms (genetic, simplex or simulated annealing algorithms) demonstrated to be very effective in femtosecond lasers for both parametric amplifiers pulse compression (Bartels et al, 2000), focalization (Villoresi et al, 2004), and microscopy (Wright et al, 2007). Also the capability of realizing a fast analysis of a reference point image has been successfully used in some applications .…”

Section: Introductionmentioning

confidence: 99%

Innovative Membrane Deformable Mirrors

Bonora¹,

Bortolozzo²,

Naletto³

et al. 2012

Topics in Adaptive Optics

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“…Defined as deviations from ideal optical wavefronts, aberrations can be caused by the optical imaging system or by the sample itself. Adaptive optics provides a means to correct aberrations by physically modifying the effective pupil phase profile of the objective lens, enabling significant improvements in resolution and SNR (20)(21)(22)(23)(24). Adaptive optics has enabled high-resolution imaging of the rods and cones of the human retina, with confocal microscopy (24) and OCT (25)(26)(27).…”

mentioning

confidence: 99%

Computational adaptive optics for broadband optical interferometric tomography of biological tissue

Adie¹,

Graf²,

Ahmad³

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

191

157

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Aberrations in optical microscopy reduce image resolution and contrast, and can limit imaging depth when focusing into biological samples. Static correction of aberrations may be achieved through appropriate lens design, but this approach does not offer the flexibility of simultaneously correcting aberrations for all imaging depths, nor the adaptability to correct for sample-specific aberrations for high-quality tomographic optical imaging. Incorporation of adaptive optics (AO) methods have demonstrated considerable improvement in optical image contrast and resolution in noninterferometric microscopy techniques, as well as in optical coherence tomography. Here we present a method to correct aberrations in a tomogram rather than the beam of a broadband optical interferometry system. Based on Fourier optics principles, we correct aberrations of a virtual pupil using Zernike polynomials. When used in conjunction with the computed imaging method interferometric synthetic aperture microscopy, this computational AO enables object reconstruction (within the single scattering limit) with ideal focal-plane resolution at all depths. Tomographic reconstructions of tissue phantoms containing subresolution titanium-dioxide particles and of ex vivo rat lung tissue demonstrate aberration correction in datasets acquired with a highly astigmatic illumination beam. These results also demonstrate that imaging with an aberrated astigmatic beam provides the advantage of a more uniform depth-dependent signal compared to imaging with a standard Gaussian beam. With further work, computational AO could enable the replacement of complicated and expensive optical hardware components with algorithms implemented on a standard desktop computer, making high-resolution 3D interferometric tomography accessible to a wider group of users and nonspecialists.low-coherence tomography | three-dimensional microscopy | aberration compensation | holography | inverse scattering

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Adaptive optics for enhanced signal in CARS microscopy

Cited by 97 publications

References 29 publications

Effect of scattering on coherent anti-Stokes Raman scattering (CARS) signals

Effect of scattering on coherent anti-Stokes Raman scattering (CARS) signals

Innovative Membrane Deformable Mirrors

Computational adaptive optics for broadband optical interferometric tomography of biological tissue

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