Adaptive Strategies and Pathogenesis of Clostridium difficile from
            <i>In Vivo</i>
            Transcriptomics

Janoir, Claire; Denève, Cécile; Bouttier, Sylvie; Barbut, Frédéric; Hoÿs, Sandra; Caleechum, Laxmee; Chapeton-Montes, Diana; Pereira, Fátima C.; Henriques, Adriano O.; Collignon, Anne; Monot, Marc; Dupuy, Bruno

doi:10.1128/iai.01408-13

Cited by 18 publications

(36 citation statements)

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“…Accordingly, we found that σ B positively controls genes encoding surface‐associated proteins that may be involved in adhesion to epithelial cells or to extracellular‐matrix components. Among these genes, four genes, slpA , cwp27/CD0440 , cwp19/CD2767 and CD3145 are expressed early during colonisation in axenic mice, and their expression decreases at a late stage of infection, suggesting that these genes may be involved in the early stage of colonisation (Janoir et al ., ).…”

Section: Resultsmentioning

confidence: 97%

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The alternative sigma factor σ^B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

Kint

Janoir

Monot

et al. 2017

Environmental Microbiology

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170

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Clostridium difficile is a major cause of diarrhoea associated with antibiotherapy. Exposed to stresses in the gut, C. difficile can survive by inducing protection, detoxification and repair systems. In several firmicutes, most of these systems are controlled by the general stress response involving σ . In this work, we studied the role of σ in the physiopathology of C. difficile. We showed that the survival of the sigB mutant during the stationary phase was reduced. Using a transcriptome analysis, we showed that σ controls the expression of ∼25% of genes including genes involved in sporulation, metabolism, cell surface biogenesis and the management of stresses. By contrast, σ does not control toxin gene expression. In agreement with the up-regulation of sporulation genes, the sporulation efficiency is higher in the sigB mutant than in the wild-type strain. sigB inactivation also led to increased sensitivity to acidification, cationic antimicrobial peptides, nitric oxide and ROS. In addition, we showed for the first time that σ also plays a crucial role in oxygen tolerance in this strict anaerobe. Finally, we demonstrated that the fitness of colonisation by the sigB mutant is greatly affected in a dixenic mouse model of colonisation when compared to the wild-type strain.

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Section: Resultsmentioning

confidence: 97%

“…Sporulation is rapidly induced during the colonisation process in axenic mice (Janoir et al ., ). Therefore, we monitored the number of spores in faecal and caecal contents as well as in the caecal mucosa (Supporting Information Fig.…”

Section: Resultsmentioning

confidence: 97%

The alternative sigma factor σ^B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

Kint

Janoir

Monot

et al. 2017

Environmental Microbiology

Self Cite

170

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“…Nevertheless, the use of a germ‐free mouse model can have important applications as recently shown by Janoir et al . (). These researchers utilised germ‐free C3H mice to study the C. difficile transcriptome over the course of infection (Janoir et al ., ).…”

Section: Gnotobiotic Mouse Models Used To Study CDImentioning

confidence: 97%

Small animal models for the study ofClostridium difficiledisease pathogenesis

Hutton

Mackin

Chakravorty

et al. 2014

FEMS Microbiol Lett

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Clostridium difficile is the leading cause of bacterial antibiotic-associated diarrhoea in hospitals in the developed world. Despite this notoriety, the complex mechanisms employed by this pathogen to overcome innate host defences and induce fulminant disease are poorly understood. Various animal models have been used extensively for C. difficile research to study disease pathogenesis. Until recently, the most commonly used C. difficile disease model has utilised hamsters; however, mouse and pig models have now been developed that unravel different aspects of C. difficile pathology. This review summarises key aspects of the small animal models currently used in C. difficile studies with a specific focus on major differences between them. Furthermore, this review highlights the advantages and disadvantages of each model and illustrates that careful consideration is required when selecting models for use in C. difficile research.

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“…Si bien los cuatro factores sigma están presentes en los genomas que hasta la fecha han sido secuenciados de C. difficile, y los cambios morfológicos durante el desarrollo de la espora son similares a los de B. subtilis, la regulación temporal difiere significativamente de este segundo 35 . Estudios transcriptómicos han demostrado que C. difficile comienza a esporular cuatro hs después de iniciada la infección en un modelo murino de IACD, observándose esporas maduras 14 h después de este inicio 36 . Recientemente, Deakinet y cols.…”

Section: Formación De La Espora De C Difficile Durante La Iacdunclassified

“…A pesar de que no existe evidencia directa de esporulación y persistencia de esporas de C. difficile en el tracto colónico de humanos, es posible que estos procesos sean similares a los reportados en modelos murinos 36,37 . Estos datos concuerdan con estudios clínicos donde 50% de los pacientes que se recuperan de un episodio de IACD se convierten en diseminadores asintomáticos de sus esporas por un período de 1-4 semanas después de la antibioterapia 26,38 .…”

Section: Formación De La Espora De C Difficile Durante La Iacdunclassified

Esporas de Clostridium difficile y su relevancia en la persistencia y transmisión de la infección

Barra-Carrasco

Hernández-Rocha

Ibáñez

et al. 2014

Rev. chil. infectol.

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Clostridium difficile spores and its relevance in the persistence and transmission of the infection C. difficile is an anaerobic spore former pathogen and the most important etiologic agent of nosocomial and community acquired antibiotics associated diarrheas. C. difficile infections (CDI) are responsible for an elevated rate of morbidity in developed and developing countries. Although the major virulence factors responsible for clinical symptoms of CDI are the two toxins TcdA and TcdB, C. difficile spores are the main vehicle of infection, persistence and transmission of CDI. Recent work has unrevealed unique properties of C. difficile spores that make them remarkable morphotypes of persistence and transmission in the host, including their resistance to antibiotics, the host immune response and disinfectants. The present review summarizes relevant aspects of C. difficile spore biology that have major implications from a clinical and medical perspective.

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Adaptive Strategies and Pathogenesis of Clostridium difficile from In Vivo Transcriptomics

Cited by 18 publications

References 0 publications

The alternative sigma factor σ^B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

The alternative sigma factor σ^B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

Small animal models for the study ofClostridium difficiledisease pathogenesis

Esporas de Clostridium difficile y su relevancia en la persistencia y transmisión de la infección

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Adaptive Strategies and Pathogenesis of Clostridium difficile from In Vivo Transcriptomics

Cited by 18 publications

References 0 publications

The alternative sigma factor σB plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

The alternative sigma factor σB plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

Small animal models for the study ofClostridium difficiledisease pathogenesis

Esporas de Clostridium difficile y su relevancia en la persistencia y transmisión de la infección

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The alternative sigma factor σ^B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection

The alternative sigma factor σ^B plays a crucial role in adaptive strategies of Clostridium difficile during gut infection