Adaptive Variations of Sediment Microbial Communities and Indication of Fecal-Associated Bacteria to Nutrients in a Regulated Urban River

Abstract

Anthropogenic activities strongly influence river habitat conditions and surrounding landscape patterns. A major challenge is to understand how these changes impact microbial community composition and structure. Here, a comprehensive analysis combining physicochemical characteristics in sediment with sequencing targeting the V4 region of the 16S rRNA gene was conducted to test the hypothesis that diverse habitat conditions induce dissimilarity of microbial community composition and structure in a regulated urban river. The results suggested that observed species richness and Shannon–Wiener diversity had a decreasing variation along the land use intensified gradient, while beta diversity also revealed significant separation of microbial community structure between headwaters and urban reaches. Total nitrogen (TN), total phosphorus (TP), oxidation–reduction potential (ORP) and total organic carbon (TOC) in sediment were the dominant factors in structuring bacterial and archaeal community assemblages. Further analysis in dominant fecal-associated bacteria indicated that elevated nutrient concentrations may significantly (p < 0.05) increase the relative abundance of Clostridium and Acinetobacter in sediment. The findings highlight the pivotal roles of alpha diversity and fecal-associated bacteria in understanding the dynamics of microbial communities in a regulated urban river ecosystem.

Structural Diversity of Bacterial Communities and Its Relation to Environmental Factors in the Surface Sediments from Main Stream of Qingshui River

“…The southern part belongs to a warm temperate semi-humid zone, the middle part belongs to a middle temperate semiarid zone, and the northern part belongs to a middle temperate arid zone [16]. Existing research has focused on the Guyuan section in the south of the Qingshui River and the confluence of the Yellow River in the north [24,25]. The overall understanding of bacteria in the Qingshui River is still lacking.…”

Insights on the particle-attached riverine archaeal community shifts linked to seasons and to multipollution during a Mediterranean extreme storm event

“…Archaea have been studied in only a few river environments (Herfort et al 2009;Hu et al 2016Hu et al , 2018Cannon et al 2017;Samson et al 2019;Lei et al 2020;Cao et al 2020;Pinto et al 2020;Shen et al 2021) and to the best of our knowledge, no study has characterized their diversity over different seasons in lotic ecosystems. Here, eDNA was extracted from riverine water samples from summer, autumn, and winter and characterized using high-throughput…”

“…Among the few papers in the literature that have linked archaeal diversity to environmental parameters, all addressed the effect of one family of parameters on changes in archaeal communities, such as nutrients (Herfort et al 2009;Hu et al 2016Hu et al , 2018Lei et al 2020;Cao et al 2020) or metals (Mahamoud Ahmed et al 2020;Shen et al 2021). This is, therefore, the first study on the Archaea domain where both diversity and a large panoply of physicochemical parameters (notably nutrients, trace metals, pharmaceuticals, and pesticides) have been analyzed for the same samples.…”

Variability of the structure of winter microbial communities in Chelyabinsk lakes

“…The rate at which urban areas around cities grow are exceeding the rate of population growth (Li et al, 2021), which worsens the hygienic properties of fresh water and, consequently, human health (Xie et al, 2021). Anthropogenic transformation of lakes leads to the introduction of non-typical bacteria (including fecal) into the water systems and can change the natural composition of the microbial community of freshwater (Numberger et al, 2019;Cao et al, 2020;Yang et al, 2020). Normally, communities of natural microorganisms are able to limit the growth of pathogens sporadically polluting water bodies.…”