Adding by subtracting : Impairing tomato susceptibility genes to obtain resistance to Verticillium wilt

Hanika, Katharina

doi:10.18174/531120

Cited by 2 publications

(3 citation statements)

References 210 publications

(449 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…dahliae resistance in tomato (Hanika, 2020), auxin signalling and transport mutants showed enhanced resistance against Fusarium oxysporum in Arabidopsis (Kidd et al, 2011;Lyons et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Auxins have also been implicated in susceptibility to vascular pathogens. While auxin receptor and transporter mutants of Arabidopsis show enhanced resistance to V. dahliae (Fousia et al ., 2018), and deletion of the vacuolar auxin transporter WAT1 resulted in enhanced V. dahliae resistance in tomato (Hanika, 2020), auxin signalling and transport mutants showed enhanced resistance against Fusarium oxysporum in Arabidopsis (Kidd et al ., 2011; Lyons et al ., 2015).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

A singleVerticillium dahliaeeffector determines pathogenicity on tomato by targeting auxin response factors

Faino

Fiorin

et al. 2022

Preprint

View full text Add to dashboard Cite

Verticillium dahliae is a xylem-invading fungal pathogen that causes devastating vascular wilt diseases on hundreds of plant hosts, including tomato (Solanum lycopersicum). Although individual V. dahliae strains are typically characterized by their broad host range, differential pathogenicity occurs on nearly all hosts. Currently, the molecular basis underlying such pathogenicity differences remains unknown. We used comparative genomics to identify a single effector gene that specifically occurs in tomato-pathogenic V. dahliae strains and is expressed during tomato colonization. Functional analyses showed that this Tom1 effector governs pathogenicity on tomato, as Tom1 deletion prohibited tomato colonization, while introduction of Tom1 into non-pathogenic V. dahliae or into saprophytic sister species V. tricorpus and V. nubilum resulted in disease. Through proteomics-based approaches, auxin response factors (ARFs) were identified as in planta targets of Tom1. Intriguingly, repression of SlARF2a expression by virus-induced gene silencing fully impaired V. dahliae colonization of tomato, solidifying its role as susceptibility target.Collectively, our findings indicate that a single effector, Tom1, mediates pathogenicity of V. dahliae on tomato by targeting auxin response factors.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A singleVerticillium dahliaeeffector determines pathogenicity on tomato by targeting auxin response factors

Faino

Fiorin

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Chapter 5 axr4 revealed that these plants display reduced symptoms and less V. dahliae biomass accumulation when compared with wild-type plants (Fousia et al, 2018). Furthermore, WAT1, a vacuolar auxin transporter, was demonstrated to play an important role in tomato susceptibility to V. dahliae, since targeted deletion of its encoding gene lead to enhanced plant resistance (Hanika, 2020). Moreover, auxin signaling mutants arf1, arf2, axr2, axr3, and sgt1b, as well as auxin transport mutants big, axr4, aux1, and pin2 of A. thaliana showed reduced disease symptoms when challenged with the wilt pathogen F. oxysporum, although fungal biomass was not quantified (Kidd et al, 2011;Lyons et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Functional analysis of effector proteins of the vascular wilt fungus Verticillium dahliae

Fiorin

View full text Add to dashboard Cite

In order to establish disease, plant pathogens exploit effectors as molecular tools that manipulate host processes to support host colonization. Verticillium dahliae is a soil-borne fungal pathogen that causes vascular wilt disease in hundreds of dicotyledonous species. For particular hosts, V. dahliae isolates have been grouped in pathotypes based on differential aggressiveness. Based on their disease phenotypes, V. dahliae strains are typically assigned to the defoliating or the non-defoliating pathotype on cotton. A single V. dahliae effector, named the D effector, was previously demonstrated to be necessary and sufficient for the induction of defoliation by strains of the defoliating pathotype, and to act as pathogenicity determinant on this host, as well as on the model plant Arabidopsis thaliana. Thus far, the mechanism underlying D effector activity remains unknown. In this study, we used heterologously produced effector protein to investigate biochemical properties of the D effector in planta. Effector infiltration in cotton cotyledons induced yellowing, vein browning as well as necrosis, and transcript profiling showed that photosynthesis was strongly repressed, while secondary metabolism and response to stress were induced in infiltrated cotyledons. These observations indicate that the D effector triggers stress responses in cotton. In addition, we examined the effect of the D protein in cotton tissues at cellular level. Cells exhibiting reticulate cell wall reinforcements typical of tracheary elements were observed adjacent to xylem vessels and even on the epidermis, suggesting that the D effector induces ectopic xylem differentiation.

show abstract

Adding by subtracting : Impairing tomato susceptibility genes to obtain resistance to Verticillium wilt

Cited by 2 publications

References 210 publications

A singleVerticillium dahliaeeffector determines pathogenicity on tomato by targeting auxin response factors

A singleVerticillium dahliaeeffector determines pathogenicity on tomato by targeting auxin response factors

Functional analysis of effector proteins of the vascular wilt fungus Verticillium dahliae

Contact Info

Product

Resources

About