Addition of a protease to low crude protein density diets of broiler chickens

Abstract

A total of 864 1-day-old Ross 308 broiler chickens with an average body weight of 40 ± 1.1 g were used to determine the effect of adding protease to broiler chickens diets with different crude protein (CP) density on growth performance, carcase characteristics, excreta microbiota, blood constituents, and nutrient digestibility. The chicks were allotted in pens with 18 birds/pen and 12 pen/treatment. Treatments were T1: basal diet, T2: T1 − 1.0% CP, T3: T2 + 0.05% protease, T4: T2 + 0.1% protease. Results showed that supplementing low CP diets with protease alleviated the negative effects of lowering dietary CP on BWG and FCR (P < .05) on days 1-21. Addition of protease to low CP diets improved BWG on days 1-35 (P < .05). Feeding the chickens with low CP diet reduced (P < .05) digestibility of dry matter (DM), but the addition of protease improved digestibility of DM. The results showed that lowering dietary CP had detrimental influence (P < .05) on digestibility of total essential amino acids (TEAA) and total nonessential amino acids (TNEAA). It is concluded that reduction of dietary CP had a negative effect on broiler chickens and the addition of protease alleviated the negative effects and improved chickens performance.

Effects of dietary protein levels and protease supplementation on growth performance, carcass traits, meat quality, and standardized ileal digestibility of amino acid in Pekin ducks fed a complex diet

“… Farhat and Chavez (1999) observed changes in breast muscle thickness using ultrasound showing values of 8.42, 7.26, and 6.93 mm for high (23%), medium (19%), and low protein (17%) programs, respectively. Mohammadigheisar et al. (2018) found that feeding broiler chickens with LP diets had no effect on breast meat yield.…”

“…(2002) found that supplemented protease had been applied to LP diets without deleterious effects on the performance of broiler chickens. Supplemental exogenous proteases have been found to improve protein digestibility, energy efficiency, and performance indices in broilers ( Fru-Nji et al., 2011 ), even in a LP diet ( Cowieson et al., 2017 , Lei et al., 2017 , Morales et al., 2017 , Law et al., 2018 , Mohammadigheisar and Kim, 2018 ), which indicated that reducing dietary CP levels with exogenous supplementation can be used for production. However, to our knowledge, no data are available concerning the supplementation of protease to reduced CP levels of diets in ducks.…”

Effect of Enzymes Application in Feed with Reduced Concentrations of Energy and Crude Protein in Broiler Chickens

“…반대로 xylanase를 사 료에 배합하였을 때 Lactobacillus 등 장내 균총 변화에 영향 을 미치지 않았다 (Lu et al, 2009). 또한, 본 연구와 유사한 이 전 연구에서 조단백질 수준을 낮추고, 프로테아제 효소제를 급여하는 실험에서도 마찬가지로 총균수, 유산균수, 대장균수 에서 유의적인 차이는 발생하지 않았다 (Mohammadigheisar and Kim, 2018 2 Control diet, balanced diet; T1, diet with reduction of 100 kcal ME/kg and 1% of CP and addition of 4,000 μ/g xylanase, 400 μ/g α-amylase, and 8,000 μ/g protease; T2, diet with reduction of 100 kcal ME/kg and 1% of CP and addition of 800 μ/g βmannanase; T3, diet with reduction of 100 kcal ME/kg and 1% of CP and addition of 2,200 μ/g xylanase and 200 μ/g βglucanase; T4, diet with reduction of 100 kcal ME/kg and 1% of CP and addition of 1,000 μ/g xylanase. 3 SEM, standard error of the mean.…”

Effects of broiler chicks fed the low crude protein diet supplemented with Achyranthes japonica Nakai root extract on growth performance and carcass traits

“…In order to reduce the environmental pollution and feed cost of poultry husbandry, a method of reducing crude protein (CP) content in diet was put forward. However, it has been reported that reducing crude protein in diet has negative effects on the performance of birds (Mohammadigheisar and Kim, 2018).…”