Addition of Chitosan to Silicate Cross-Linked PEO for Tuning Osteoblast Cell Adhesion and Mineralization

Gaharwar, Akhilesh K.; Schexnailder, Patrick J.; Jin, Qiao; Wu, Chi‐Chuan; Schmidt, Gudrun

doi:10.1021/am100609t

Cited by 68 publications

(60 citation statements)

References 53 publications

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“…3 Clay consumption (geophagy) is commonly observed in reptiles, birds and mammals. 171,146 Several theories such as removal of toxins present in the diet, treatment of gastrointestinal ailments such as diarrhea via the antimicrobial components of clay minerals, mineral supplementation, and alleviating stomach hyperacidity have been suggested for the geophagic behavior of animals. 47 In many cases of geophagy, minerals from the kaolin group were identied as major components consumed among the clay minerals.…”

Section: Cationic-cm In Animal Healthmentioning

confidence: 99%

Biomedical applications of cationic clay minerals

2015

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Clay minerals have been a subject of interest owing to their ready availability in nature, a wide range of applications in various industries, and particularly their current and potential biomedical applications.They have been widely used for curative and protective purposes by humans since ancient times.Cationic clay minerals possess specific physicochemical characteristics such as high surface reactivity (high adsorption, cation exchange, colloidal or swelling capacity), good rheological behavior, high acidabsorbing capacity, and high dispersibility in water, which renders them suitable for various biomedical applications. Only a few reviews have exclusively discussed the biomedical applications of clay minerals, and to our knowledge, there is no updated review focusing on cationic clay minerals and their applications in pharmaceuticals, cosmetics, and regenerative medicine. In this review, we provide a brief introduction on natural, synthetic, and hybrid cationic clay minerals followed by a detailed discussion about their applications in biological systems.

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Section: Cationic-cm In Animal Healthmentioning

confidence: 99%

Biomedical applications of cationic clay minerals

2015

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“…5,15,16 Gaharwar et al used chitosan to modify the material properties of PEO-silicate gels to tune cellular adhesion and control biomineralization on a cell-biomaterial interface. 17 Many investigators have also evaluated cell-specific properties on gels made by different crosslinkers. [18][19][20] The multifunctional gels created previously, as summarized by Leach et al, 21 depict the potential of current hydrogels in controlling cellular behavior, integration of engineered materials with host or transplanted tissue, and inductive factor presentation.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Pandit

Zuidema

Venuto

et al. 2013

Tissue Engineering Part A

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The use of hydrogels for bone regeneration has been limited due to their inherent low modulus to support cell adhesion and proliferation as well as their susceptibility to bacterial infections at the wound site. To overcome these limitations, we evaluated multifunctional polysaccharide hydrogels of varying stiffness to obtain the optimum stiffness at which the gels (1) induce proliferation of human dermal fibroblasts, human umbilical vascular endothelial cells (HUVECs), and murine preosteoblasts (MC3T3-E1), (2) induce osteoblast differentiation and mineralization, and (3) exhibit an antibacterial activity. Rheological studies demonstrated that the stiffness of hydrogels made of a polysaccharide blend of methylcellulose, chitosan, and agarose was increased by crosslinking the chitosan component to different extents with increasing amounts of genipin. The gelation time decreased (from 210 to 60 min) with increasing genipin concentrations. Proliferation of HUVECs decreased by 10.7 times with increasing gel stiffness, in contrast to fibroblasts and osteoblasts, where it increased with gel stiffness by 6.37 and 7.8 times, respectively. At day 14 up to day 24, osteoblast expression of differentiation markers-osteocalcin, osteopontin-and early mineralization marker-alkaline phosphatase, were significantly enhanced in the 0.5% (w/v) crosslinked gel, which also demonstrated enhanced mineralization by day 25. The antibacterial efficacy of the hydrogels decreased with the increasing degree of crosslinking as demonstrated by biofilm formation experiments, but gels crosslinked with 0.5% (w/v) genipin still demonstrated significant bacterial inhibition. Based on these results, gels crosslinked with 0.5% (w/v) genipin, where 33% of available groups on chitosan were crosslinked, exhibited a stiffness of 502 -64.5 Pa and demonstrated the optimal characteristics to support bone regeneration.

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“…While charged chitosan enhanced cell adhesion and growth, PEO inhibited protein and cell adhesion. Additionally, Laponite and chitosan‐based NC hydrogel promoted the differentiation of osteoblast cells showing that this scaffold can be further used for bone repair studies . Another Laponite/PEO NC hydrogel as biomaterials was described by the same group.…”

Section: Nanocomposite Hydrogels Of Nanomaterials As Biomaterialsmentioning

confidence: 98%

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

2014

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In biotechnology, SAMs and NC hydrogels of functional nanomaterials are of high interest as 2D and 3D cell culture systems, respectively, to mimic natural ECM and to control cell behaviors. Advanced biotechnological approaches often use nanoscale topography together with suitable surface functionalization, as a model of ECM, to control cell behaviors and tissue formation. SAMs of NMs are effective ECM models as 2D surfaces due to their larger nanostructured surface areas which provide higher molecular density by functionalization on a planar substrate than molecular SAMs. Additionally, NC hydrogels, produced by cross-linking of organic polymers with NMs, are excellent candidates as 3D cell culture systems owing to their optical, cell-compatibility and the extraordinary mechanical properties.

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Addition of Chitosan to Silicate Cross-Linked PEO for Tuning Osteoblast Cell Adhesion and Mineralization

Cited by 68 publications

References 53 publications

Biomedical applications of cationic clay minerals

Biomedical applications of cationic clay minerals

Evaluation of Multifunctional Polysaccharide Hydrogels with Varying Stiffness for Bone Tissue Engineering

Self-assembled Monolayers and Nanocomposite Hydrogels of Functional Nanomaterials for Tissue Engineering Applications

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