Addition of conjugated linoleic acid in whole milk improves lipid profile in high fat diet induced hypercholesterolemia of rats

Abstract

185 Farmawati et al., Addition of conjugated linoleic acid in whole milk improves lipid profilein high fat diet induced hypercholesterolemia of rats J Med Sci, Volume 48, No. 4, 2016 October: 185-192 ABSTRACTConjugated linoleic acid (CLA) is an isomer of linoleic acid that has been shown to have many beneficial effects in prevention of atherosclerosis, hypertension, cardiovascular diseases and improve immune function. Although majority of CLA in the diet are derived from dairy product such as milk, however, the content of CLA in milk is affected by cow's diet. The aim of this study was to investigate the beneficial effect of CLA supplementation in milk for improving lipid profile in high fat diet of rats. Twenty four male Sprague Dawley rats aged 8 weeks were given high fat diet for 3 weeks to induce hypercholesterolemia. Six rats were maintained in standard diet as control. Rats then were divided into 4 groups i.e. normal control, negative control, high fat diet+CLA 0.5%, high fat diet + CLA 0.5% supplemented skim milk, and high fat diet + CLA 0.5% supplemented whole milk. Blood sample was drawn after high fat diet induced hypercholesterolemia and after 4 weeks of treatment for total cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL cholesterol), and high density lipoprotein cholesterol (HDL cholesterol) analysis. Body weight was measured each week. Results showed that body weight was significantly increase in all groups received high fat diet (p<0.05). There was no significant difference in body weight between treatment group (p>0.05). Total cholesterol, triglyceride, and LDL cholesterol was significantly decrease in whole milk followed by significant increase in HDL cholesterol level. Skim milk supplemented with CLA had only modest effect on triglyceride and HDL cholesterol level. In conclusion, CLA supplementation in whole milk improves lipid profile in high fat diet. ABSTRAKAsam linoleat terkonjugasi adalah isomer asam linoleat yang terbukti mempunyai manfaat dalam mencegah aterosklerosis, hipertensi, penyakit kardiovaskuler dan meningkatkan sistem imun. Meskipun sebagian besar asam linoleat dalam diet berasal dari produk makanan sehari-hari seperti susu, namum demikian kandungan asam linoleat dalam susu dipengaruhi oleh diet sapi penghasil susu. Penelitian ini bertujuan untuk mengkaji manfaat asam linoleat dalam suplemen susus dalam memperbaiki profil lipid pada tikus diet lemak tinggi. Dua puluh empat tikus jantan Sprague Dawly berumur 8 minggu diberi diet lemak tinggi selama 3 minggu untuk menginduksi hiperkolesterolemia. Enam ekor tikus diberi diet makan standar sebagai control. Tikus kemudian dibagi menjadi empat kelompok yaitu kelompok kontrol normal, kelompok kontrol negatif, kelompok diet tinggi lemak dengan J Med Sci, Volume 48, No. 4, 2016 October: 185-192 186 asam linoleat 0.5%, kelompok diet tinggi lemak dengan asam linoleat 0.5% dalam suplemen susu skim, kelompok diet tinggi lemak dengan asam linoleat 0.5% dalam suplemen susu susu murni. Sampel darah diambim setelah di...