Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

Evaluation of Cartilage Repair by Mesenchymal Stem Cells Seeded on a PEOT/PBT Scaffold in an Osteochondral Defect

“…Furthermore, the MSCs were seen to be attached to the scaffold struts and distributed throughout the 3D construct as proposed by Jansen for optimal cartilage repair 6 . With respect to the MSC differentiation behaviour in vitro, GAG accumuluation after 21 days culture in the presence of TGFβ 3 in both normoxic and hypoxic conditions compared well to other studies 3,18 , with GAG/DNA values in hypoxia twice that observed in normoxia.…”

Development of a gene-activated scaffold platform for tissue engineering applications using chitosan-pDNA nanoparticles on collagen-based scaffolds

“…At pre-determined time-points, fluorescent microscopy was also used to visualise cells expressing GFP using a Leica DMIL microscope (Leica Microsystems, Switzerland) and version 3. Three different collagen-based scaffolds -collagen alone, collagen-hydroxyapatite (CHA) [35] and collagen-hyaluronic acid (CHyA) [36] were fabricated using a lyophilisation technique developed by O'Brien et al [39]. The collagen slurry was made by adding 1.8 g of bovine tendon collagen (Integra Life Sciences, USA) to 360 mL of 0.5M glacial acetic acid (HOAc) and blending at 15,000 rpm for 90 mins using an overhead blender (Ultra Turrax T18 Overhead Blended, IKA Works Inc., USA) at a constant temperature of 4°C.…”

“…This type of chitosan has not been tested on MSCs previously but has shown potential in cell lines such as HEK293 cells and cervical cancer (HeLa) cells as well as in vivo in corneal and retinal applications [26,31,32]. The optimal chitosan nanoparticle formulations were then applied to three different collagen-based scaffolds; collagen, collagen hydroxyapatite (CHA) and collagen hyaluronic acid (CHyA) which have previously been developed within our lab for repair of bone and cartilage [33][34][35][36] and the transfection efficiencies of the gene-activated scaffolds were determined.…”

A biomimetic multi-layered collagen-based scaffold for osteochondral repair

“…This study set out to develop a collagen-based multi-layered scaffold with distinct but seamlessly integrated layers that mimic the structure and composition of osteochondral tissue while also showing potential for use clinically as a cell-free scaffold that would allow the attachment, proliferation and infiltration of the host's own cells recruited from the bone marrow while also providing an optimised intermediate layer, seamlessly integrated and designed to mimic the calcified cartilaginous interface once implanted in vivo. We hypothesised that an ideal scaffold for osteochondral repair could be produced by combining a base layer consisting of a collagen hydroxyapatite scaffold exhibiting osteoinductive properties and potential for bone repair [29] with a collagen-HA-glycosaminoglycan intermediate calcified cartilage layer, and finally a pro-chondrogenic collagen-hyaluronic acid based cartilaginous layer [30].…”