2012
DOI: 10.1016/j.bej.2012.04.010
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Addition of protease during starch liquefaction affects free amino nitrogen, fusel alcohols and ethanol production of fermented maize and whole and decorticated sorghum mashes

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Cited by 29 publications
(13 citation statements)
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“…Then, hydrolyzates were saccharified with amyloglucosidase (Dextrozyme, Novozymes, Bagsvaerd, Denmark) in preparation for fermentation trials. The saccharification was performed at 60°C during 17 h in an incubator shaker (VWR International, Model RF1575) and the worts were diluted with distilled water to 12 or 20°P concentrations [ 21 ]. The gravity (expressed as °P) was measured using a digital refractometer (CATAGO HRS 500, Bellevue, WA, USA).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation
“…Then, hydrolyzates were saccharified with amyloglucosidase (Dextrozyme, Novozymes, Bagsvaerd, Denmark) in preparation for fermentation trials. The saccharification was performed at 60°C during 17 h in an incubator shaker (VWR International, Model RF1575) and the worts were diluted with distilled water to 12 or 20°P concentrations [ 21 ]. The gravity (expressed as °P) was measured using a digital refractometer (CATAGO HRS 500, Bellevue, WA, USA).…”
Section: Methodsmentioning
confidence: 99%
“…After the °Plato (°P, gravity) concentration was adjusted, the worts were pasteurized in a water bath and transferred to sterilized Erlenmeyer flasks without additional supplementation. Finally, worts were inoculated in sterile conditions (Labconco Model 36212, Kansas City, MI, USA) and incubated at 30°C without mixing for 72 h fermentation [ 21 ].…”
Section: Methodsmentioning
confidence: 99%
“…The ethanol production is based on the quantity of consumed sugars in fermented wort (17), and thus is positively correlated with wort fermentability. In the present study, the sugar supplementation improving wort fermentability could increase the ethanol production ( Fig.…”
Section: Fermentation Characteristicsmentioning
confidence: 99%
“…Fumi et al also argued that maize‐adjunct worts have a lower total nitrogen compound content than all‐malt worts and free amino nitrogen is nearly double that in all‐malt compared with adjunct worts. However, Perez‐Carrillo et al argued that the free nitrogen source content in wort increased as a result of added protease, and the concentration of the free nitrogen source in corn wort (60%) was twice that found in sorghum wort (30%). Starch granules in the corn endosperm are embedded in a continuous protein matrix and enclosed in highly pitted thin walls ; therefore, researchers are investigating techniques to enhance the conversion of corn starch into fermentable sugar.…”
Section: Introductionmentioning
confidence: 99%