Additions to The Knowledge of Tubakia (Tubakiaceae, Diaporthales) in China

Abstract

The species of Tubakia (Tubakiaceae, Diaporthales, Sordariomycetes) have often been reported as endophytes and pathogens on woody plants. During the investigation of Tubakia species from Fagaceae trees in China, 46 isolates were obtained from diseased leaves and seeds. The characterization of these isolates was based on the observation of morphological characters, the effect of temperature on mycelial growth rate, as well as the combined genes of ITS, tef1 and tub2. As a result, six species were identified: Tubakia americana, T. cyclobalanopsidis sp. nov., T. dryinoides, T. koreana, T. paradryinoides and T. quercicola sp. nov. Among these, T. koreana and T. paradryinoides were firstly discovered in China. Pathogenicity tests were conducted using the conidial suspension on young, excised leaves for these six species, which showed that they were mildly pathogenic to four Fagacece hosts: C. mollissima, Q. acutissima, Q. aliena var. acutiserrata and Q. variabilis.

Phaeotubakia lithocarpicola gen. et sp. nov. (Tubakiaceae, Diaporthales) from leaf spots in China

“…China and Japan, where Fagaceae hosts are the most common hosts (Harrington and McNew 2018). Obovoideisporodochium lithocarpi and several new Tubakia species (T. cyclobalanopsidis and T. quercicola) recently discovered from trees of Fagaceae (Zhang et al 2021;Zhu et al 2022), and Phaeotubakia lithocarpicola proposed in the present study support this phenomenon well. More taxa of Tubakiaceae may be revealed by more investigations of fungal diversity on Fagaceae in the future.…”

“…Phaeotubakia is distinguished from Apiognomonioides, Ellipsoidisporodochium, Involutiscutellula, Oblongisporothyrium, Obovoideisporodochium, Paratubakia, Racheliella, Saprothyrium and Sphaerosporithyrium by having brown to dark brown conidia (Braun et al 2018;Zhang et al 2021). Several species of Tubakia are known to have brown conidia, which is similar to Phaeotubakia lithocarpicola (Braun et al 2018;Zhu et al 2022). However, they are phylogenetically distinct (Fig.…”

“…Conidia of Apiognomonioides, Ellipsoidisporodochium, Oblongisporothyrium, Obovoideisporodochium and Saprothyrium species are known to be hyaline (Braun et al 2018;Zhang et al 2021;Liu et al 2022). Conidia of Involutiscutellula, Paratubakia and Sphaerosporithyrium species are hyaline to slightly pigmented (Braun et al 2018), while conidia of Racheliella and Tubakia species are hyaline to pigmented (Braun et al 2014(Braun et al , 2018Zhu et al 2022).…”

“…Tubakiaceae species are known to be endophytes in leaves and twigs of many tree species, but can also cause conspicuous symptoms on host leaves as plant pathogens (Harrington et al 2012;Braun et al 2018;Zhu et al 2022). Nearly all tubakiaceous species are reported from Fagaceae, such as species of Castanea, Castanopsis, Fagus, Lithocarpus and Quercus (Braun et al 2018;Morales-Rodríguez et al 2021).…”

Diversity of Monochaetia Species from Fagaceous Leaf Spots in China and Pathogenicity for Chinese Chestnut

“…Fungal species diversity occurring on fagaceous leaves has been recently studied based on morphological and phylogenetic features, revealing many interesting and new species of Gnomoniopsis , Neopestalotiopsis , Pestalotiopsis , Tubakia , and other genera ( 3 – 6 ). Monochaetia is a pestalotioid genus that was initially recognized as a subgenus of Pestalotia (as Pestolozzia ), being characterized by the presence of a single apical appendage ( 7 ).…”