Additive cell protective and oxidative stress reducing effects of combined treatment with cromolyn sodium and masitinib on MPTP-induced toxicity in SH-SY5Y neuroblastoma cells

Erol, Azize Yasemin Göksu; Koçanci, Fatma Gonca; Dora, Devrim Demir; Uysal, Hilmi

doi:10.1016/j.cbi.2022.109808

Cited by 5 publications

(5 citation statements)

References 53 publications

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“…10 mg MPTP was dissolved in 1 ml of double distilled water. The solution was filter-sterilized and stored in dark at À20 C. Concentration titration assay of MPTP, including four different concentrations, as 100, 500, 1000, and 1500 μM, for cell viability was performed, as described in our previous study (Goksu Erol et al, 2022).…”

Section: Reagents and Chemicalsmentioning

confidence: 99%

“…Among these various concentrations, 1000 μM was selected as it yielded an approximate survival rate of 65-70% in (d)-SH-SY5Y cells (Data not shown). This concentration was subsequently employed in our experimental applications to establish our in vitro neurodegenerative models (Goksu Erol et al, 2022).…”

Section: Sh-sy5y Cellsmentioning

confidence: 99%

“…Collectively, these advantages have led us to test the protective properties of LentiVIP gene delivery against microglial toxicity and toxin‐mediated neurodegeneration on an in vitro neurodegenerative model. In this context, we constructed a neurodegenerative model by treating neuron‐like human differentiated (d)‐SH‐SY5Y cells with 1‐methyl‐4‐phenyl‐1,2,3,6‐tetrahydropyridine (MPTP), which is frequently used to build ‘in vivo’ and ‘in vitro’ PD models (Chen et al, 2018; Goksu Erol et al, 2022). This toxin is metabolized into the toxic cation 1‐methyl‐4‐phenylpyridinium (MPP + ) by the enzyme, monoamine oxidase B (MAO‐B) (Frim et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

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Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Goksu,

Kocanci,

Akinci

et al. 2024

Eur J of Neuroscience

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A common pathological hallmark of neurodegenerative disorders is neuronal cell death, accompanied by neuroinflammation and oxidative stress. The vasoactive intestinal peptide (VIP) is a pleiotropic peptide that combines neuroprotective and immunomodulatory actions. The gene therapy field shows long‐term promise for treating a wide range of neurodegenerative diseases (ND). In this study, we aimed to investigate the in vitro efficacy of transduction of microglia using lentiviral gene therapy vectors encoding VIP (LentiVIP). Additionally, we tested the protective effects of the secretome derived from LentiVIP‐infected “immortalized human” microglia HMC3 cells, and cells treated with Synthetic VIP (SynVIP), against toxin‐induced neurodegeneration. First, LentiVIP, which stably expresses VIP, was generated and purified. VIP secretion in microglial conditioned media (MG CM) for LentiVIP‐infected HMC3 microglia cells was confirmed. Microglia cells were activated with lipopolysaccharide, and groups were formed as follows: 1) Control, 2) SynVIP‐treated, or 3) LentiVIP‐transduced. These MG CM were applied on an in vitro neurodegenerative model formed by differentiated (d)‐SH‐SY5Y cells. Then, cell survival analysis and apoptotic nuclear staining, besides measurement of oxidative/inflammatory parameters in CM of cells were performed. Activated MG CM reduced survival rates of both control and toxin‐applied (d)‐SH‐SY5Y cells, whereas LentiVIP‐infected MG CM and SynVIP‐treated ones exhibited better survival rates. These findings were supported by apoptotic nuclear evaluations of (d)‐SH‐SY5Y cells, alongside oxidative/inflammatory parameters in their CM. LentiVIP seems worthy of further studies for the treatment of ND because of the potential of gene therapy to treat diseases effectively with a single injection.

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Section: Reagents and Chemicalsmentioning

confidence: 99%

Section: Sh-sy5y Cellsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Goksu,

Kocanci,

Akinci

et al. 2024

Eur J of Neuroscience

Self Cite

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“…29,31,32 In the differentiated form, SH-SY5Y cells exhibit morphological similarities to human neurons, such as increased polarization, elongation of neurites, and the formation of functional synapses. 33,34 Moreover, differentiation of SH-SY5Y cells results in their withdrawal from the cell cycle, leading to a homogeneous population of neuronal cells. 29,31,35 Thus, SH-SY5Y cells are extensively employed in screening studies to evaluate the neurotrophic properties and neurotoxicity of pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, SH‐SY5Y cells can differentiate into a more mature human neuronal phenotype 29,31,32 . In the differentiated form, SH‐SY5Y cells exhibit morphological similarities to human neurons, such as increased polarization, elongation of neurites, and the formation of functional synapses 33,34 . Moreover, differentiation of SH‐SY5Y cells results in their withdrawal from the cell cycle, leading to a homogeneous population of neuronal cells 29,31,35 .…”

Section: Introductionmentioning

confidence: 99%

Beyond boundaries: Neuroprotective effects of steroids and ecdysteroids in SH‐SY5Y cells ‐ A systematic review

Tayeb,

Njangiru,

Minorics

2024

Neuroprotection

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Steroids and ecdysteroids have been shown to exhibit a range of biological effects, including anti‐inflammatory, anticancer, and neuroprotective. This systematic review aims to highlight the evidence‐based neuroprotective and antioxidant effects of steroids and ecdysteroids in SH‐SY5Y neuroblastoma cells. A comprehensive literature search was conducted on May 11, 2023, without publication source restrictions, using various electronic databases, including PubMed, Web of Science (WoS), Scopus, and Cumulative Index to Nursing and Allied Health Literature. Of 103 articles, only 20 studies were included for investigating the neuroprotective effects of steroids and ecdysteroids in SH‐SY5Y cells exposed to oxidative stress or neurotoxic agents. The risk of bias and quality assessment of the included studies were evaluated in accordance with the Nature Publication Quality Improvement Project specific criteria. The selected studies reported the antioxidant effects of the tested compounds on SH‐SY5Y cells and demonstrated their ability to scavenge free radicals and prevent lipid peroxidation. These findings suggest that neurosteroids have potential therapeutic applications for the prevention and treatment of neurodegenerative diseases characterized by oxidative stress and neuronal damage. Some studies have investigated the molecular mechanisms underlying the neuroprotective and antioxidant effects of steroids and ecdysteroids in SH‐SY5Y cells. These mechanisms include the activation of antioxidant enzymes, such as superoxide dismutase and glutathione peroxidase, and the modulation of various signaling pathways, including the phosphoinositide 3‐kinase/protein kinase B and mitogen‐activated protein kinase/extracellular signal‐regulated kinase pathways. This review provides evidence that the tested compounds have remarkable neuroprotective and antioxidant effects in human neuroblastoma SH‐SY5Y cells.

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A review article on the development of dopaminergic neurons and establishment of dopaminergic neuron–based in vitro models by using immortal cell lines or stem cells to study and treat Parkinson's disease

Göksu

2024

Intl J of Devlp Neuroscience

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The primary pathological hallmark of Parkinson's disease (PD) is the degeneration of dopaminergic (DA) neurons in the substantia nigra pars compacta, a critical midbrain region. In vitro models based on DA neurons provide a powerful platform for investigating the cellular and molecular mechanisms of PD and testing novel therapeutic strategies. A deep understanding of DA neuron development, including the signalling pathways and transcription factors involved, is essential for advancing PD research. This article first explores the differentiation and maturation processes of DA neurons in the midbrain, detailing the relevant signalling pathways. It then compares various in vitro models, including primary cells, immortalized cell lines, and stem cell‐based models, focusing on the advantages and limitations of each. Special attention is given to the role of immortalized and stem cell models in PD research. This review aims to guide researchers in selecting the most appropriate model for their specific research goals. Ethical considerations and clinical implications of using stem cells in PD research are also discussed.

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Additive cell protective and oxidative stress reducing effects of combined treatment with cromolyn sodium and masitinib on MPTP-induced toxicity in SH-SY5Y neuroblastoma cells

Cited by 5 publications

References 53 publications

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Microglia cells treated with synthetic vasoactive intestinal peptide or transduced with LentiVIP protect neuronal cells against degeneration

Beyond boundaries: Neuroprotective effects of steroids and ecdysteroids in SH‐SY5Y cells ‐ A systematic review

A review article on the development of dopaminergic neurons and establishment of dopaminergic neuron–based in vitro models by using immortal cell lines or stem cells to study and treat Parkinson's disease

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