Additive Effect of Vitamin K2 and Risedronate on Long Bone Mass in Hypophysectomized Young Rats

Iwamoto, Jun; Takeda, Tsuyoshi; Satō, Yoshihiro; Yeh, James K.

doi:10.1538/expanim.56.103

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…RAL (Amoun Co, Nasr City, Cairo, Egypt) was dissolved in 20% w/v hydroxypropyl‐ß‐cyclodextrin (Sigma –Aldrich chemie Gmbh, Steinheim, Germany) and given orally by stomach tube to rats (3 mg/kg bw, three times/week) . RIS (Alkan, Pharma Co, October City, Egypt) was dissolved in sterile saline and given subcutaneously at 5 μg/kg bw, twice/week . Control ORX group was given the vehicles (1 mL of 20% w/v hydroxypropyl‐ß‐cyclodextrin by stomach tube three times/week and 0.3 mL sterile saline subcutaneously twice/week).…”

Section: Methodsmentioning

confidence: 99%

Modulation of bone turnover in orchidectomized rats treated with raloxifene and risedronate

Khedr

El-Ashmawy

El-Bahrawy

et al. 2012

Fundamemntal Clinical Pharma

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Osteoporosis is a reduction in bone mineral density (BMD). It develops less often in men than in women. This study aimed to evaluate the bone protective effects of raloxifene (RAL), risedronate (RIS), and their combination on osteoporotic male rats. Forty male Wister rats (12 weeks) were randomly divided into five groups: sham-operated group (n = 8), orchidectomized (ORX) group (n = 7), RAL group (n = 9), RIS group (n = 7) and RAL + RIS group (n = 7). RAL was orally administered at 3 mg/kg three times/week, and RIS was given subcutaneously at 5 μg/kg, twice weekly. After 6 weeks of treatment, serum cathepsin-K, alkaline (ALP) and acid phosphatase activities, serum osteocalcin, serum Ca²⁺, and Pi were determined. Urinary Ca²⁺ and deoxypyridinoline levels, BMD, and Ca²⁺ content of femur ash were estimated. Histochemical localization of ALP activity of tibia and histomorphometry was examined. As compared to sham, ORX rats showed a significant increase in bone turnover markers, and histochemical activity of ALP was increased markedly in proximal tibia of ORX rats, whereas BMD and Ca²⁺ content of femur ash were reduced after ORX. These changes were modulated after treatment with RAL and RIS or both to ORX rats; BMD of femur was improved by each treatment, and bone turnover markers were reduced as compared to ORX vehicle group. We concluded that orchidectomy induced osteoporosis and increased bone turnover in male rats because of withdrawal of sex hormones. Both RAL and RIS could treat osteoporosis in ORX rats; they reduced bone turnover markers and maintained BMD.

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Section: Methodsmentioning

confidence: 99%

Modulation of bone turnover in orchidectomized rats treated with raloxifene and risedronate

Khedr

El-Ashmawy

El-Bahrawy

et al. 2012

Fundamemntal Clinical Pharma

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“…Furthermore, as with vitamin K2, DHNA has a naphthoquinone skeleton. Vitamin K2 is produced by microbes and improves bone metabolism by facilitating calcium fixation [32]. Since DHNA has a naphthoquinone skeleton, we assumed that like vitamin K2, DHNA would be therapeutically effective for osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

Suppressive effects of 1,4-dihydroxy-2-naphthoic acid administration on bone resorption

et al. 2009

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Effects of combination treatment with alendronate and vitamin K2 on bone mineral density and strength in ovariectomized mice

et al. 2010

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Bisphosphonates increase bone mineral density (BMD) by suppressing remodeling space and elongating the duration of mineralization. Menatetrenone (vitamin K(2)) reduces the incidence of fractures by improving bone quality through enhanced gamma-carboxylation of bone glutamic acid residues of osteocalcin in osteoporotic patients. This study investigated the effects of combination treatment with alendronate (ALN) and vitamin K(2) on BMD and bone strength in ovariectomized (OVX) mice. Thirty-three female mice, 16 weeks of age, were assigned to four groups: (1) OVX-control group; (2) oral vitamin K(2) group; (3) subcutaneous ALN group; and (4) ALN + vitamin K(2) group. The treatment was started 4 weeks after OVX and continued for 4 weeks. BMD, geometric parameters measured by peripheral quantitative computed tomography, and mechanical strength at the femoral metaphysis and mid-diaphysis were evaluated after an 8-week treatment period. ALN alone significantly increased total BMD (20%, P < 0.05) and trabecular BMD (25%, P < 0.05), but not the mechanical parameters of the femur, compared with the OVX-control group. Combination treatment with ALN and vitamin K(2) increased not only total BMD (15%, P < 0.05) and trabecular BMD (32%, P < 0.05) but also maximum load (33%, P < 0.05) and breaking energy (25%, P < 0.05) of compression test at the distal metaphysis, and maximum load (20%, P < 0.05) and breaking force (33%, P < 0.05) of three-point bending test at the mid-diaphysis compared with the OVX-control group. These results suggest that ALN, alone or in combination with vitamin K(2), showed significant improvement in BMD, but that the combination treatment was more effective than ALN alone for improving bone strength in OVX mice.

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Additive Effect of Vitamin K2 and Risedronate on Long Bone Mass in Hypophysectomized Young Rats

Abstract: Hypophysectomy (HX) arrests bone growth and induces osteopenia in the long

Cited by 5 publications

References 42 publications

Modulation of bone turnover in orchidectomized rats treated with raloxifene and risedronate

Modulation of bone turnover in orchidectomized rats treated with raloxifene and risedronate

Suppressive effects of 1,4-dihydroxy-2-naphthoic acid administration on bone resorption

Effects of combination treatment with alendronate and vitamin K2 on bone mineral density and strength in ovariectomized mice

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