Additive manufacturing of natural biopolymers and composites for bone tissue engineering

Bose, Susmita; Koski, Caitlin; Vu, Ashley A.

doi:10.1039/d0mh00277a

Cited by 92 publications

(47 citation statements)

References 97 publications

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“…Treatment of bone defects are a big challenge for modern medicine. The biggest thing of research in biomaterials for tissue engineering is to design and prepare suitable three dimensional scaffold and finding a new, adequate solutions is extremely important ( Figure 2 ) [ 34 ]. The latest trend in biomedical engineering is the search for mixtures based on natural polymers that would demonstrate compatibility with human tissues [ 35 ].…”

Section: Introductionmentioning

confidence: 99%

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

et al. 2021

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Blending of different biopolymers, e.g., collagen, chitosan, silk fibroin and cross-linking modifications of these mixtures can lead to new materials with improved physico-chemical properties, compared to single-component scaffolds. Three-dimensional scaffolds based on three-component mixtures of silk fibroin, collagen and chitosan, chemically cross-linked, were prepared and their physico-chemical and biological properties were evaluated. A mixture of EDC (N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride) and NHS (N-hydroxysuccinimide) was used as a cross-linking agent. FTIR was used to observe the position of the peaks characteristic for collagen, chitosan and silk fibroin. The following properties depending on the scaffold structure were studied: swelling behavior, liquid uptake, moisture content, porosity, density, and mechanical parameters. Scanning Electron Microscopy imaging was performed. Additionally, the biological properties of these materials were assessed, by metabolic activity assay. The results showed that the three-component mixtures, cross-linked by EDC/NHS and prepared by lyophilization method, presented porous structures. They were characterized by a high swelling degree. The composition of scaffolds has an influence on mechanical properties. All of the studied materials were cytocompatible with MG-63 osteoblast-like cells.

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Section: Introductionmentioning

confidence: 99%

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

et al. 2021

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“…Over the last few years, additive manufacturing (AD) approaches have also gained a lot of attention as an alternative fabrication route to organize cells in 3D due to their potential to provide precise spatiotemporal control over biophysical and biochemical cues necessary to reproduce a biomimetic microenvironment (Murr, 2016 ; Bose et al, 2020 ; Nikolaev et al, 2020 ; Sun et al, 2020 ). AD approaches include techniques such as three-dimensional printing (3DP) ( Figures 4D,E ), light-assisted bioprinting (Trautmann et al, 2018 ), fused deposition modeling (FDM), selective laser sintering (SLS), that, along with advances in biomaterials and bioinks, enable precise deposition of materials into custom shapes and patterns to replicate complex tissue architectures, not possible using conventional techniques, and with high control and reproducibility (Melchels et al, 2012 ; Cui et al, 2017 ).…”

Section: Building Blocks For Developing Human Tissue Equivalentsmentioning

confidence: 99%

Advances in Engineering Human Tissue Models

Moysidou

Barberio

Owens

2021

Front. Bioeng. Biotechnol.

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Research in cell biology greatly relies on cell-based in vitro assays and models that facilitate the investigation and understanding of specific biological events and processes under different conditions. The quality of such experimental models and particularly the level at which they represent cell behavior in the native tissue, is of critical importance for our understanding of cell interactions within tissues and organs. Conventionally, in vitro models are based on experimental manipulation of mammalian cells, grown as monolayers on flat, two-dimensional (2D) substrates. Despite the amazing progress and discoveries achieved with flat biology models, our ability to translate biological insights has been limited, since the 2D environment does not reflect the physiological behavior of cells in real tissues. Advances in 3D cell biology and engineering have led to the development of a new generation of cell culture formats that can better recapitulate the in vivo microenvironment, allowing us to examine cells and their interactions in a more biomimetic context. Modern biomedical research has at its disposal novel technological approaches that promote development of more sophisticated and robust tissue engineering in vitro models, including scaffold- or hydrogel-based formats, organotypic cultures, and organs-on-chips. Even though such systems are necessarily simplified to capture a particular range of physiology, their ability to model specific processes of human biology is greatly valued for their potential to close the gap between conventional animal studies and human (patho-) physiology. Here, we review recent advances in 3D biomimetic cultures, focusing on the technological bricks available to develop more physiologically relevant in vitro models of human tissues. By highlighting applications and examples of several physiological and disease models, we identify the limitations and challenges which the field needs to address in order to more effectively incorporate synthetic biomimetic culture platforms into biomedical research.

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“…Several strategies, such as electrowriting, electrospinning, and AM, have been developed and being utilized to model hierarchical tissue engineering. [ 60–63 ] Electrowriting and electrospinning use an applied voltage to induce the collection of a stable fluid on a collector plate. The main difference between these two methods is the electrical instabilities caused by the applied voltage.…”

Section: Fabrication Tools and Techniquesmentioning

confidence: 99%

Recent Advances in Regenerative Tissue Fabrication: Tools, Materials, and Microenvironment in Hierarchical Aspects

Ratri

Brilian

Setiawati

et al. 2021

Advanced NanoBiomed Research

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As part of regenerative medicine, artificial, hierarchical tissue engineering is a favorable approach to satisfy the needs of patients for new tissues and organs to replace those with defects caused by age, disease, or trauma or to correct congenital disabilities. However, the application of tissue engineering faces critical issues, such as the biocompatibility of the fabricated tissues and organs, the scaffolding, the complex biomechanical processes within cells, and the regulation of cell biology. Although fabrication strategies, including the traditional bioprinting, photolithography, and organ‐on‐a‐chip methods, as well as combinations of fabrication processes, face many challenges, they are methods that can be used in hierarchical tissue engineering. The strategic approach to synthetic, hierarchical tissue engineering is to use a combination of several technologies incorporating material science, cell biology, additive manufacturing (AM), on‐a‐chip strategies, and biomechanics. Herein, in a review, the current materials and biofabrication strategies of various artificial hierarchical tissues are discussed based on the level of tissue complexity from nano to macrosize and the adaptive interactions between cells and the scaffolding surrounding the incorporated cells.

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Additive manufacturing of natural biopolymers and composites for bone tissue engineering

Abstract:
Through additive manufacturing (AM) of natural biopolymers, significant progress has been made in the field of biomedical devices and bone tissue engineering of low load bearing applications like maxillofacial, bone defects, and dental.

Cited by 92 publications

References 97 publications

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

Advances in Engineering Human Tissue Models

Recent Advances in Regenerative Tissue Fabrication: Tools, Materials, and Microenvironment in Hierarchical Aspects

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Additive manufacturing of natural biopolymers and composites for bone tissue engineering

Abstract: Through additive manufacturing (AM) of natural biopolymers, significant progress has been made in the field of biomedical devices and bone tissue engineering of low load bearing applications like maxillofacial, bone defects, and dental.

Cited by 92 publications

References 97 publications

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

Biomaterials with Potential Use in Bone Tissue Regeneration—Collagen/Chitosan/Silk Fibroin Scaffolds Cross-Linked by EDC/NHS

Advances in Engineering Human Tissue Models

Recent Advances in Regenerative Tissue Fabrication: Tools, Materials, and Microenvironment in Hierarchical Aspects

Contact Info

Product

Resources

About

Abstract:
Through additive manufacturing (AM) of natural biopolymers, significant progress has been made in the field of biomedical devices and bone tissue engineering of low load bearing applications like maxillofacial, bone defects, and dental.