Using the linear elastic finite element method, we investigated how defects significantly influence the integrity of 3D-printed parts made from biodegradable material by experimental techniques and numerical simulations. A defective flaw was incorporated into the tensile test dog-bone sample using Computer-Aided Design and processed by slicing software. Three distinct raster angles examine two sets of samples, one featuring intact specimens and the other with the introduced defects. An open-source 3D printer was used to fabricate both sets of samples, utilizing biodegradable PLA material. In finite element analysis, we employed a highly detailed model that precisely accounted for the geometry and dimensions of the extruded 3D-printed filament, accurately replicating the actual configuration of the 3D-printed samples to an extent. Our study involved a thorough comparative analysis between the experimental results and the FEA simulations. Our findings uncovered a consistent trend for the intact and defective samples under tensile load. Specifically, in the intact case, the samples with a zero-degree raster orientation presented the highest resistance to failure and displayed minimal elongation. Remarkably, these conclusions paralleled our observations of the defective samples as well. Finite element analysis revealed that the stresses, including Principal, Max shear, and Von Mises, were remarkably higher at the 3D-printed samples’ outer surface than the inner layers, reflecting that the failure starts at the outer surface since they exceeded the theoretical values, indicating a significant discrepancy between the simulated and anticipated values.