2017
DOI: 10.1039/c7sc03362a
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Addressing the autofluorescence issue in deep tissue imaging by two-photon microscopy: the significance of far-red emitting dyes

Abstract: We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.

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Cited by 150 publications
(136 citation statements)
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“…As mentioned above, TPFM overcomes autofluorescence from intrinsic biomolecules using TPA-active dyes with emission maximaa bove 625 nm. [4] It is knownt hat planarization and p-expansion of ac hromophore often resultsi nt he bathochromic shift of absorption and emission maxima. Our recent reports have shown an elegant approach for the expansiono f the diketopyrrolopyrrole p-system, based on ad ouble intramolecular Friedel-Crafts reaction.…”
Section: Design and Synthesismentioning
confidence: 99%
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“…As mentioned above, TPFM overcomes autofluorescence from intrinsic biomolecules using TPA-active dyes with emission maximaa bove 625 nm. [4] It is knownt hat planarization and p-expansion of ac hromophore often resultsi nt he bathochromic shift of absorption and emission maxima. Our recent reports have shown an elegant approach for the expansiono f the diketopyrrolopyrrole p-system, based on ad ouble intramolecular Friedel-Crafts reaction.…”
Section: Design and Synthesismentioning
confidence: 99%
“…Secondly, TPFM overcomes autofluorescence issues intrinsic to biomolecules due to the long excitation wavelengths. Thirdly, it grants an unprecedented imaging depth in the order of hundreds of micrometers whilst allowing three‐dimensional resolution at the submicrometer level.…”
Section: Introductionmentioning
confidence: 99%
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“…AF can be attributed to biomolecules such as NADH, folic acid, and retinol which have emission maxima in the range of 450-500 nm, and riboflavin, flavin coenzymes, and flavoproteins which have emission maxima in the range of 520-540 nm. 18 . AF creates a background that can impair the detection of dim markers emitting light at the same wavelengths In spectral flow cytometry, the spectral profile of unstained cells can be collected and treated as an independent parameter (AF), which allows the AF signature to be extracted using the unmixing algorithm.…”
Section: D) Autofluorescence (Af)mentioning
confidence: 99%
“…Finally,weevaluated the ability of DMONs-PEI to deliver mCherry mRNA( with the same length to EGFP mRNA) in vivo in BALB/c mice to avoid the autofluorescence interference. [20] Strong mCherry signal was detected after 48 hi na xillary,i nguinal and mesenteric lymph nodes for DMONs-PEI-mCherry mRNAt reated mice,w hile in vivo-jetPEI group treated mice exhibited fluorescence signal mainly at injection site and limited in lymph nodes (Figure 4A). Thea verage radiant efficiencya ts ignal sites was calculated and plotted in Figure 4B,w here DMONs-PEI exhibited comparable radiant efficiency to in vivo-jetPEI.…”
mentioning
confidence: 97%