AdeG efflux pump as the main Tigecycline resistance in Acinetobacter baumannii

Abbas, Jalileh Ebn; Salimizand, Himen; Hassanzadeh, Sepideh; Ramazanzadeh, Rashid

doi:10.1016/j.genrep.2020.100689

Cited by 7 publications

(8 citation statements)

References 23 publications

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“…Furthermore, it is documented that A . baumannii can use other efflux pump-associated genes [ 14 , 65 ] and superfamily [ 22 ] to develop a multidrug-resistance profile. In addition, researchers reported that the AdeABC efflux pump need, in some cases, a synergistic action with other resistance mechanisms to express a level of resistance to particular antibiotic classes [ 67 ].…”

Section: Discussionmentioning

confidence: 99%

“…Literature supports that it is not just the presence of the AdeABC efflux pump-associated gene but their overexpression triggered by a mutation that induce resistance to antibiotics [15,63,64]. Furthermore, it is documented that A. baumannii can use other efflux pumpassociated genes [14,65] and superfamily [22] to develop a multidrug-resistance profile. In addition, researchers reported that the AdeABC efflux pump need, in some cases, a synergistic action with other resistance mechanisms to express a level of resistance to particular antibiotic classes [67].…”

Section: Discussionmentioning

confidence: 99%

“…A . baumannii has demonstrated several antimicrobial resistance mechanisms [ 8 – 10 ] against antibiotics such as aminoglycosides, carbapenems, fluoroquinolones, cephalosporins, tetracyclines, sulbactams, rifampicins [ 9 ], as well as colistin [ 11 – 13 ] and tigecycline [ 8 , 14 ], the so called last resort drugs used to treat its infections [ 8 , 11 – 14 ]. Moreover, literature has reported resistance to recently developed antibiotics such as eravacycline [ 15 ] and cefiderocol [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…e bacterium causes pneumonia, bacteraemia, septicaemia [5], meningitis [6], and urinary tract infections [7] often seen in patients undergoing invasive procedures such as urinary catheter, tracheal intubation, and those with underlying conditions [2]. A. baumannii has demonstrated several antimicrobial resistance mechanisms [8][9][10] against antibiotics such as aminoglycosides, carbapenems, fluoroquinolones, cephalosporins, tetracyclines, sulbactams, rifampicins [9], as well as colistin [11][12][13] and tigecycline [8,14], the so called last resort drugs used to treat its infections [8,[11][12][13][14]. Moreover, literature has reported resistance to recently developed antibiotics such as eravacycline [15] and cefiderocol [16].…”

Section: Introductionmentioning

confidence: 99%

“…e overexpression of efflux pumps in A. baumannii has been associated with an increased resistance to antibiotics such as tigecycline [14,23], carbapenems [24], minocycline, gentamycin, doxycycline, and tetracycline [25]. Several researchers support that among the resistancenodulation-cell division superfamily, the adverse effect of ATP-binding cassette (AdeABC) is the most associated with resistance in A. baumannii [26,27].…”

Section: Introductionmentioning

confidence: 99%

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Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Nogbou

Nkawane

Ntshane

et al. 2021

International Journal of Microbiology

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Acinetobacter baumannii (A. baumannii) has developed several resistance mechanisms. The bacteria have been reported as origin of multiple outbreaks. This study aims to investigate the use of efflux pumps and quinolone resistance-associated genotypic mutations as mechanisms of resistance in A. baumannii isolates at a tertiary hospital. A total number of 103 A. baumannii isolates were investigated after identification and antimicrobial susceptibility testing by VITEK2 followed by PCR amplification of blaOXA-51. Conventional PCR amplification of the AdeABC efflux pump (adeB, adeS, and adeR) and quinolone (parC and gyrA) resistance genes were performed, followed by quantitative real-time PCR of AdeABC efflux pump genes. Phenotypic evaluation of efflux pump expression was performed by determining the difference between the MIC of tigecycline before and after exposure to an efflux pump inhibitor. The Sanger sequencing method was used to sequence the parC and gyrA amplicons. A phylogenetic tree was drawn using MEGA 4.0 to evaluate evolutionary relatedness of the strains. All the collected isolates were blaOXA-51-positive. High resistance to almost all the tested antibiotics was observed. Efflux pump was found in 75% of isolates as a mechanism of resistance. The study detected parC gene mutation in 60% and gyrA gene mutation in 85%, while 37% of isolates had mutations on both genes. A minimal evolutionary distance between the isolates was reported. The use of the AdeABC efflux pump system as an active mechanism of resistance combined with point mutation mainly in gyrA was shown to contribute to broaden the resistance spectrum of A. baumannii isolates.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Nogbou

Nkawane

Ntshane

et al. 2021

International Journal of Microbiology

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Antimicrobial resistance and biofilm formation capacity among Acinetobacter baumannii strains isolated from patients with burns and ventilator‐associated pneumonia

Khoshnood

Sadeghifard

Mahdian

et al. 2022

Clinical Laboratory Analysis

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Background Acinetobacter baumannii is a pathogen responsible for nosocomial infections, especially in patients with burns and ventilator‐associated pneumonia (VAP). The aims of this study was to compare the biofilm formation capacity, antimicrobial resistance patterns and molecular typing based on PFGE (Pulsed‐Field Gel Electrophoresis) in A. baumannii isolated from burn and VAP patients. Materials and Methods A total of 50 A. baumannii isolates were obtained from burn and VAP patients. In this study, we assessed antimicrobial susceptibility, biofilm formation capacity, PFGE fingerprinting, and the distribution of biofilm‐related genes (csuD, csuE, ptk, ataA, and ompA). Results Overall, 74% of the strains were multidrug resistant (MDR), and 26% were extensively drug‐resistant (XDR). Regarding biofilm formation capacity, 52%, 36%, and 12% of the isolates were strong, moderate, and weak biofilm producers. Strong biofilm formation capacity significantly correlated with XDR phenotype (12/13, 92.3%). All the isolates harbored at least one biofilm‐related gene. The most prevalent gene was csuD (98%), followed by ptk (90%), ataA (88%), ompA (86%), and csuE (86%). Harboring all the biofilm‐related genes was significantly associated with XDR phenotype. Finally, PFGE clustering revealed 6 clusters, among which cluster No. 2 showed a significant correlation with strong biofilm formation and XDR phenotype. Conclusion Our findings revealed the variable distribution of biofilm‐related genes among MDR and XDR A. baumannii isolates from burn and VAP patients. A significant correlation was found between strong biofilm formation capacity and XDR phenotype. Finally, our results suggested that XDR phenotype was predominant among strong‐biofilm producer A. baumannii in our region.

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AdeABC, AdeFGH, and AdeIJK efflux pumps as key factors in tigecycline resistance of Acinetobacter baumannii: a study from Western Balkan hospitals

Novović,

Radovanović,

Gajić

et al. 2024

Eur J Clin Microbiol Infect Dis

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AdeG efflux pump as the main Tigecycline resistance in Acinetobacter baumannii

Cited by 7 publications

References 23 publications

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Efflux Pump Activity and Mutations Driving Multidrug Resistance in Acinetobacter baumannii at a Tertiary Hospital in Pretoria, South Africa

Antimicrobial resistance and biofilm formation capacity among Acinetobacter baumannii strains isolated from patients with burns and ventilator‐associated pneumonia

AdeABC, AdeFGH, and AdeIJK efflux pumps as key factors in tigecycline resistance of Acinetobacter baumannii: a study from Western Balkan hospitals

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