Adenine Arabinoside Monophosphate (Vidarabine Phosphate) in Combination with Human Leukocyte Interferon in the Treatment of Chronic Hepatitis B

García, Gabriel; Smith, Coleman I.; Weissberg, Jed; Eisenberg, Mark; Bissett, Jack; Nair, Prem V.; Mastre, Barbara; Rosno, S; Roskamp, Deborah; Waterman, Karen

doi:10.7326/0003-4819-107-2-278

Cited by 82 publications

(14 citation statements)

References 23 publications

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“…This difference is probably explained by the nature of the medium and its effects on hepatocytes and/ or other cells. Many antiviral compounds have been evaluated as possible therapeutical agents for chronic hepatitis B (14,15). The pyrophosphate analog PFA and the 5'-triphosphates of the family of 2'-fluoroarabinosyl pyrimidine analogs (FIAC, FMAU, FEAU) had been found to be efficient inhibitors of hepadnavirus DNA polymerase in uitro (16,17).…”

Section: Discussionmentioning

confidence: 99%

Prolonged duck hepatitis B virus replication in duck hepatocytes cocultivated with rat epithelial cells: A useful system for antiviral testing

et al. 1989

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Duck cultured hepatocytes from Pekin ducks naturally infected by duck hepatitis B virus can remain functional twice longer if a coculture system with rat liver epithelial cells is used instead of ordinary primary culture. The use of a selective medium in which ornithine and lactate replaced arginine and glucose, respectively, allowed viral replication initiated in vivo to be maintained in the coculture for 2 months. Several antiviral compounds including the pyrophosphate analog (phosphonoformic acid) or nucleoside analogs (9 beta-arabinofuranosyl AMP, 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-iodocytosine, 1,2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-5-ethyluracil and 1,2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl thymine) were studied in both culture systems for their ability to inhibit duck hepatitis B virus replication. Hepatocytes were treated for 7 days with 1,2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl-5-ethyluracil (10 microM) and 1,2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl thymine (0.5 microM) or for 14 days with 9 beta-arabinofuranosyl AMP (90 microM), phosphonoformic acid (100 microM) and 1-(2'-deoxy-2'-fluoro-beta-D-arabinofuranosyl)-5-iodocytosine (6 microM). The effects of the drugs on viral replication were monitored by testing for duck hepatitis B virus DNA in the culture supernatant and in the cells by molecular hybridization.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 99%

Prolonged duck hepatitis B virus replication in duck hepatocytes cocultivated with rat epithelial cells: A useful system for antiviral testing

et al. 1989

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“…The use of the polymerase chain reaction (PCR), in addition to serological assays, has shown that the presence of HCV RNA in serum is a marker of infection, infectivity, and viral replication [Weiner 0 1992 WILEY-LISS, INC. Kaneko et al, 1990;Okamoto et al, 1990;Garson et al, 1990;Chayama et al, 1991; Farci et al, 19911. Analogous to the role of hepatitis B virus (HBV) DNA during the course of infection with HBV [Hadziyannis et al, 1983;Kaneko, 1985;Matsuyama et al, 1985;Garcia et al, 1987;Kuhns et al, 1989;Hoofnagle et al, 1988; Perrillo et al, 19901, the amount of HCV RNA may also correlate with the progression of hepatitis and may be a prognostic marker of treatment. Thus, the availability of an assay for quantitation of HCV RNA is important.…”

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confidence: 99%

Quantitation of hepatitis C virus RNA by competitive polymerase chain reaction

Kaneko

Murakami

Unoura

et al. 1992

Journal of Medical Virology

100

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Since the amount of hepatitis C virus (HCV) RNA might be correlated with the degree of severity of hepatitis and response to treatment, quantitation of HCV RNA in serum was established using competitive polymerase chain reaction (PCR). Known amounts of a plasmid containing HCV-cDNA were co-amplified with a standard dilution series of a competitive template which shared the primers' sequences but differed from the wild type cDNA in having a deletion. Accurate quantitation was obtained by comparing the amount of both products. Quantitation of serum HCV RNA was carried out in two patients' serum samples which were also used to infect chimpanzees. The concentration of HCV RNA in these two sera was calculated to be 1 pg/ml (non-infectious at 10(-3) dilution) and 1-10 pg/ml (infectious at 10(-5) dilution). The procedure was subsequently used to analyze serial changes in serum HCV RNA in three patients who were treated with alpha-interferon. During treatment, the levels of alanine aminotransferase showed a significant decrease in all patients and the amount of HCV RNA fell from 1 fg/ml, 1 pg/ml, and greater than 10 pg/ml to 0.1 fg/ml, 100 fg/ml, and 1 pg/ml, respectively. The decrease in the amount of HCV RNA after treatment was related to the initial amount of serum HCV RNA. These results suggest that quantitation of HCV RNA may be useful not only for understanding the course of HCV infection but also for evaluating treatment for HCV infection.

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“…Clinical trials reported varying rates of sustained response (Perrillo et al, 1985;Trepo et al, 1984). It became apparent that longer term dosing was associated, as reported with several other nucleoside analogues, with significant neurotoxicity and the development of peripheral neuropathy (Garcia et al, 1987). 2) is a minus enantiomer and it is thought that this may help explain the very low rates of side-effects noted with this agent.…”

Section: Nucleoside Analoguesmentioning

confidence: 99%

Treatment of Chronic Viral Hepatitis

Main

McCarron

1998

Antivir Chem Chemother

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Interferon-α therapy has proved effective for up to 40% of patients with adult-acquired chronic hepatitis B virus (HBV) infection and for 20-25% of those with chronic hepatitis C virus (HCV) infection. Nucleoside analogues, such as lamivudine and famciclovir, are showing promise as antiviral agents for chronic HBV and the combination of interferon-α and ribavirin is proving to be successful therapy for 40-50% of patients with chronic HCV. In this article we review current therapy and discuss future strategies of the therapy of chronic viral hepatitis.

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Adenine Arabinoside Monophosphate (Vidarabine Phosphate) in Combination with Human Leukocyte Interferon in the Treatment of Chronic Hepatitis B

Cited by 82 publications

References 23 publications

Prolonged duck hepatitis B virus replication in duck hepatocytes cocultivated with rat epithelial cells: A useful system for antiviral testing

Prolonged duck hepatitis B virus replication in duck hepatocytes cocultivated with rat epithelial cells: A useful system for antiviral testing

Quantitation of hepatitis C virus RNA by competitive polymerase chain reaction

Treatment of Chronic Viral Hepatitis

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