Adenine Nucleotide Levels of Canine Kidneys during Hypothermic Aerobic or Anaerobic Storage in Collins’ Solution

Abstract

The influence of retrograde oxygen persufflation (ROP) on renal adenine nucleotide, glucose and lactate contents was examined in canine kidneys during up to 5 days of hypothermic preservation. Organs with or without predamage in 30-min normothermic ischemia were preserved and the results compared to kidneys after hypothermic ischemic storage (HIS): ROP preservation resulted in a significantly higher maintenance of cellular energy-rich substances than in HIS with even normal adenine nucleotide contents and energy charge potentials for the first 24 h. As in HIS preservation of normal kidneys, where the total adenine nucleotide content (SAN) paralleled the loss of viability for the first 1–2 days, in ROP were SAN and even the lactate content also correlated to renal viability for 2–3 days. But in contrast to this correlation, which would favor adenine nucleotide determinations for viability testing, in predamaged kidneys no correlation of SAN or lactate with renal viability was found during HIS or ROP.Thus the determination of cellular energy-rich adenine nucleotides and lactate accumulation confirms the superiority of ROP over HIS preservation. But the SAN or lactate tests lose their prognostic value for posttransplant renal viability if normothermic ischemia precedes the preservation period.

Cold Ischaemic Injury in Kidney Transplantation

“…The gas is then allowed to escape through small perforations in the surface of the organ. Reports of its application date back to the 1970s [110,111]. Experimentally, there has been renewed interest in this technique showing a beneficial effect on graft function when compared to CS and HMP techniques [112,113].…”

Simulation of free radical reactions in biology and medicine: A new two-compartment kinetic model of intracellular lipid peroxidation☆

“…Superoxide ions (O 2 -) are thought to be generated by the action of xanthine oxidase upon accumulated xanthine or hypoxanthine substrates, 19 as well as by the actions of mitochondria and activated leukocytes. Both xanthine oxidase and hypoxanthine have been shown to be elevated in postischemic tissues, 19,21,22 owing both to the conversion of xanthine dehydrogenase to xanthine oxidase and to the accumulation of hypoxanthine in ischemic tissues. The hydroxyl radicals (HO) formed in reaction (3) might then attack cellular proteins (RH) and lipids (LH) directly or via intermediate radicals (R) of longer half-life (reactions 4-6).…”