Adenosine triphosphate in human semen: a quantitative estimate of fertilizing potential

Comhaire, Frank; Vermeulen, L.; Ghédira, Kamel; Más, Jorge; Irvine, Stewart; Callipolitis, Georges

doi:10.1016/s0015-0282(16)47363-2

Cited by 79 publications

(31 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition we demonstrated that isolated sperm cells contain ATP (data not shown). The presence of this nucleotide is known to be an indicator of life (10), and its measurement is used to test the fertility of human semen (4). Moreover the presence of FCR+ (positive) sperm cells in pollen which is apparently FCR negative implies that sperm cells have a stronger resistance than the pollen grains which contain them.…”

Section: Resultsmentioning

confidence: 99%

Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum

Matthys‐Rochon¹,

Vergne²,

Detchepare³

et al. 1987

Plant Physiol.

View full text Add to dashboard Cite

ABSTRACIA technical procedure is described to follow the in vitro release of the 'male germ unit' and the sperm cells from three tricellular pollen species (Brassica, Zea, and Triticum). The condition of the sperm cell was controlled using light microscopy. In addition, for the first time, the sperm cells viability has been checked by the fluorochromatic reaction test. These preliminary results indicate that this procedure appears to be a prerequisite for the successful preparation of purified viable sperm cells.In flowering plants, the sexual process of double fertilization is still relatively unknown especially the mechanisms of cell fusions. Two sperm cells (SCl and SC2)2 produced by the pollen grain are required: one fuses with the egg to form the embryo and the second fuses with the central cell to give rise to the nutritive endosperm. Today it is a challenge to understand the role played by each sperm cell. With that in mind, attempts have been made to prepare naked pollen gametophytes (sporoplasts) (12) and sperm cell nuclei (11).On the other hand, Russell (16,17) (3) were used. The details of the technical conditions are reported in Table I. RESULTS AND DISCUSSIONThe pollen species used in this work are tricellular: a vegetative cell containing two sperm cells whose nuclei may be visualized in situ by the DNA fluorochrome DAPI (Fig. 1). The isolation ofsperm cells first involves the finding ofan appropriate medium and technique to break the thick pollen wall. To obtain them free of damage, the release of intact sperm cells must be controlled throughout the isolation procedure. Finally their viability has to be verified and checked.Thus pollen grains were incubated in a BK sucrose medium to induce a light osmotic shock. For Zea and Brassica pollen a gentle grinding is coupled with the osmotic shock. In Zea pollen congestion of organelles prevents accurate observation of sperm cells which led us to find the following convenient and rapid method: using a light microscope equipped with both fluorescence and phase contrast systems to simultaneously detect the nuclei with a DNA probe (EB or DAPI) and to determine the cellular state by phase contrast microscopy. Under these conditions the release of sperm cells follows a remarkable 'time table. ' When pollen grains discharge their contents, the vegetative nucleus and the two sperm cells appear simultaneously. These three subunits are linked to form a structured set, which has previously been termed the MGU (5), demonstrated from electron microscopic data. We present the first light microscopic observation ofthe in vitro MGU in Brassica (Fig. 2). In Triticum and Zea pollens the abundance ofstorage products (starch grains) makes the MGU structure more difficult to liberate in good condition. After pollen discharge, the two sperm cells separate from the vegetative nucleus. Then they appear as elongated cells with a reduced 'active' cytoplasm, connected with a membranous tract (Fig. 3, a and b

show abstract

Section: Resultsmentioning

confidence: 99%

Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum

Matthys‐Rochon¹,

Vergne²,

Detchepare³

et al. 1987

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…Approaches have involved tests of osmoregulatory ability (Jeyendran, Van der Ven, Perez-Pelaez, Crabo & Zaneveld, 1984), metabolic status by measurement of ATP content (Comhaire et al, 1983), objective determination of quantitative and qualitative aspects of sperm motility (Overstreet, Katz, Hanson & Fonseca, 1979;Aitken et ai, 1983;Aitken, Best, Warner & Templeton, 1984), the ability of spermatozoa to penetrate bovine (Alexander, 1981;Borghi & Asch, 1983) and human (Kremer & Jager, 1982) cervical mucus, and intrinsic fertilizing ability by the use of zona pellucida-free hamster oocytes (Yanagimachi, Yanagimachi & Rogers, 1976;Yanagimachi, 1984) and human zonae pellucidae (Overstreet, Yanagimachi, Katz, Hayashi & Hanson, 1980;Overstreet, 1982;Gould et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Relationship between human sperm motility characteristics and sperm penetration into human cervical mucus in vitro

Mortimer¹,

Pandya²,

Sawers³

1986

Reproduction

136

View full text Add to dashboard Cite

“…According to the literature, seminal ATP was detected only after effective sperm membrane lysis that caused ATP to leak out [ 101. Positive correlations between ATP concentration and sperm concentration have been established as well as between ATP concentration and the performance of spermatozoa in hamster-egg-penetration tests and artificial insemination with donor by various workers [5,8,121. ATP concentration did not correlate with fertilization and could not be used by Irvine and Aitken to distinguish fertile from infertile donors in an artificial insemination program [12].…”

Section: Discussionmentioning

confidence: 99%

Sperm Adenosine Triphosphate Concentrations Before and After Freezing as a Marker of Cryolysis

Bosman¹,

Toit

Wessels

et al. 1994

Archives of Andrology

View full text Add to dashboard Cite

Adenosine triphosphate in human semen: a quantitative estimate of fertilizing potential

Cited by 79 publications

References 6 publications

Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum

Male Germ Unit Isolation from Three Tricellular Pollen Species: Brassica oleracea, Zea mays, and Triticum aestivum

Relationship between human sperm motility characteristics and sperm penetration into human cervical mucus in vitro

Sperm Adenosine Triphosphate Concentrations Before and After Freezing as a Marker of Cryolysis

Contact Info

Product

Resources

About