Adenovirus Assembly

Philipson, Lennart

doi:10.1007/978-1-4684-7935-5_8

Cited by 10 publications

(9 citation statements)

References 132 publications

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“…(1,12,16). It is also known that the 100K protein physically associates with hexon monomers in the cytoplasm (facilitating their transport into the nucleus); failure of this transport mechanism also results in the degradation of hexon monomers in the cytoplasm (4,15). We also noted that lack of 100K function also appeared to decrease the overall accumulation levels of penton and fiber proteins without affecting the relative rates of synthesis of these proteins.…”

Section: Discussionsupporting

confidence: 50%

“…Functions of the 100K protein include the transport of newly synthesized hexon monomers (the major structural protein of the Ad capsid) from the cytoplasm to the nucleus and trimerization of hexon monomers (4). Without this activity, hexon monomers are degraded in the cytoplasm (15). 100K also acts as a "scaffolding platform" for the assembly of virus capsids, although the 100K protein has not been found to be physically incorporated into mature Ad capsids (13).…”

Section: Helper Virus-independent E1-deleted Adenovirus ([E1ϫ]mentioning

confidence: 99%

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Adenovirus Vectors with the 100K Gene Deleted and Their Potential for Multiple Gene Therapy Applications

Hodges¹,

Evans

Everett³

et al. 2001

J Virol

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Section: Discussionsupporting

confidence: 50%

Section: Helper Virus-independent E1-deleted Adenovirus ([E1ϫ]mentioning

confidence: 99%

Adenovirus Vectors with the 100K Gene Deleted and Their Potential for Multiple Gene Therapy Applications

Hodges¹,

Evans

Everett³

et al. 2001

J Virol

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“…For adenovirus, only 10 to 20% of the viral structural polypeptides are assembled into new adenovirus particles, which may explain the low titer produced by this virus (23). These viral precursors, which would be present in the prepared virus stock, may consume or shield UV radiation from infectious adenovirus particles.…”

Section: Resultsmentioning

confidence: 99%

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Thurston-Enriquez

Haas

Jacangelo³

et al. 2003

Appl Environ Microbiol

238

221

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Little information regarding the effectiveness of UV radiation on the inactivation of caliciviruses and enteric adenoviruses is available. Analysis of human calicivirus resistance to disinfectants is hampered by the lack of animal or cell culture methods that can determine the viruses' infectivity. The inactivation kinetics of enteric adenovirus type 40 (AD40), coliphage MS-2, and feline calicivirus (FCV), closely related to the human caliciviruses based on nucleic acid organization and capsid architecture, were determined after exposure to low-pressure UV radiation in buffered demand-free (BDF) water at room temperature. In addition, UV disinfection experiments were also carried out in treated groundwater with FCV and AD40. AD40 was more resistant than either FCV or coliphage MS-2 in both BDF water and groundwater. The doses of UV required to achieve 99% inactivation of AD40, coliphage MS-2, and FCV in BDF water were 109, 55, and 16 mJ/cm 2 , respectively. The doses of UV required to achieve 99% inactivation of AD40, coliphage MS-2, and FCV in groundwater were slightly lower than those in BDF water. FCV was inactivated by 99% by 13 mJ/cm 2 in treated groundwater. A dose of 103 mJ/cm 2 was required for 99% inactivation of AD40 in treated groundwater. The results of this study indicate that if FCV is an adequate surrogate for human caliciviruses, then their inactivation by UV radiation is similar to those of other single-stranded RNA enteric viruses, such as poliovirus. In addition, AD40 appears to be more resistant to UV disinfection than previously reported.

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“…Both of the ITRs and the packaging signal are the cis-acting elements, which are necessary for viral DNA replication and packaging. 2,3 Transcriptional units A simplified map of the Ad5 genome with a few key landmarks is diagrammed in Figure 2. 4,5 The early (E) and late (L) regions of the genome that contain different transcription units are divided according to the onset of viral DNA replication.…”

Section: Important Cis Elementsmentioning

confidence: 99%

“…29,30 Viral particle assembly Assembly of the Ad virion generally has been described as consisting of the following steps: (a) formation of the major structural unit of capsid, (i.e., capsomers of hexon and penton), (b) assembly of empty capsids, (c) insertion of viral DNA into capsids, and (d) proteolytic cleavage of maturation. 3 During this process, the encapsidation mechanism of viral genome into viral particles is the most critical step and is of interest for the development of Ad vectors. Similar to the cis-acting packaging domains of Ad16 and Ad3 that are located within the left 390 bp, 31,32 the packaging signal of Ad5 is located in the left end of the viral genome (194 -380 bp) as seven AT-rich motifs of nucleotide sequences.…”

Section: Viral Dna Replicationmentioning

confidence: 99%

Development and application of adenoviral vectors for gene therapy of cancer

Zhang¹

1999

Cancer Gene Ther

165

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For successful gene vaccination and therapy of cancer, it is essential to develop gene delivery vectors that can meet clinical and social requirements. The need for improved vectors was clearly manifested during the peak of the first wave of gene therapy. Adenoviral (Ad) vectors have recently drawn the attention of many of those involved in the field of gene therapy for cancer because of their practical advantages and application potential. Many experiments, innovations, preclinical studies, and clinical trials have generated an overwhelming amount of data and literature concerning this vector system. It is hoped that the comprehensive review presented here, which includes the principles, potential, capacity, and limitations of the current Ad systems, will help to further the rational development of the system. The literature in this article is organized in an attempt to emphasize Ad vector development in the aspects of technical approaches, practical hurdles and strategies to overcome them, significant experimental results, recent advances, future directions, etc. The technical range of this review covers details that are intended to serve as a reference for advanced technical readers and provide a foundation for initiates in the field of Ad vector gene therapy. The material presented here is also intended for nontechnical persons who want to have an overview of the significance and potential of the technology.

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Adenovirus Assembly

Cited by 10 publications

References 132 publications

Adenovirus Vectors with the 100K Gene Deleted and Their Potential for Multiple Gene Therapy Applications

Adenovirus Vectors with the 100K Gene Deleted and Their Potential for Multiple Gene Therapy Applications

Inactivation of Feline Calicivirus and Adenovirus Type 40 by UV Radiation

Development and application of adenoviral vectors for gene therapy of cancer

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