Adenovirus genome types isolated from stools of children with gastroenteritis in São Paulo, Brazil

Hársi, Charlotte Marianna; Rolim, D P; Gomes, Saint Clair; Gilio, Alfredo Elias; Stewien, Klaus Eberhard; Baldacci, Evandro Roberto; Candeias, J. A. N.

doi:10.1002/jmv.1890450203

Cited by 31 publications

(26 citation statements)

References 29 publications

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“…Os protozoários tiveram escassa importância. Um percentual dos casos do GDA permaneceu sem identificação causal, indicando, indiretamente, que outros potenciais patógenos já reconhecidos possam estar implicados [13][14][15] . A maior parte das Escherichia coli detectadas pertencem a biovariedades freqüentemente não avaliadas rotineiramente nos laboratórios clínicos.…”

Section: Conclusãounclassified

“…O perfil etiológico da diarréia da criança tem sofrido alterações em anos recentes, com a identificação de vários microorganismos [13][14][15][16] . Além disso, outras alterações relevantes deste perfil decorreram da reavaliação do papel diarreiogênico de muitas cepas de Escherichia coli antes consideradas não patogênicas.…”

Section: Introductionunclassified

“…Alguns estudos realizados na área urbana da cidade de São Paulo avaliaram a etiologia das diarréias agudas e mostraram uma predominância dos quadros bacterianos sobre os virais 8,10,15,21,22 . Dentre os agentes bacterianos, sempre preponderou a Escherichia coli, principalmente do grupo enteropatogênico clássico (EPEC), que chegou a ser detectada em até 30 a 40% dos casos de diarréia na década de 80 8,21 .…”

Section: Introductionunclassified

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Perfil etiológico das diarréias agudas de crianças atendidas em São Paulo

Souza¹,

Martinez²,

Taddei³

et al. 2002

J. Pediatr. (Rio J.)

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Section: Conclusãounclassified

Section: Introductionunclassified

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Perfil etiológico das diarréias agudas de crianças atendidas em São Paulo

Souza¹,

Martinez²,

Taddei³

et al. 2002

J. Pediatr. (Rio J.)

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“…Un 2,8% de prevalencia a nivel nacional demuestra que este porcentaje se acerca a la prevalencia mas baja reportada en los países desarrollados que es del 2% (18,19).…”

Section: Resultsunclassified

Astrovirus (HAstV) como agente causal de diarrea en niños Colombianos: Siete años de estudio

Gutiérrez

Matiz

Ulloa

et al. 2005

nova

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ResumenCon el objeto de determinar el comportamiento de los Astrovirus como agentes causantes de diarrea en algunas regiones colombianas, se colectaron 1147 muestras de diarrea de niños menores de 5 años que acudieron a los servicios de consulta externa de cuatro centros de salud de regiones de Bogotá, Facatativa, Cartagena y Quibdo durante el periodo comprendido entre 1996 y el año 2002. La determinación de la presencia viral se realizó por medio de pruebas de ELISA encontrando una prevalencia global de 2,8%. De las muestras positivas se seleccionaron 14 a las cuales se les obtuvo su ARN, se realizaron pruebas de RT-PCR y secuenciación de un segmento de 348 pb del ORF 2 del genoma viral para elaborar dendrogramas con el método de Neiborg joining y así, determinar su comportamiento filogenético. Las conclusiones generales de este estudio fueron:que en Colombia los HAstV poseen comportamientos endémicos y que las cepas colombianas no han sufrido cambios evolutivos que les permitan distanciarse de cepas de otros países.Palabras claves: Diarrea, Astrovirus humano (HastV), RTPCR, filogenética. AbstractAstrovirus (HAstV) causing diarrea in colombian children: seven yeasr of study: In order to find the behavior of the Astrovirus isolates from diarrheic samples taken from children under 5 in four different cities of Colombian, there were tested 1147 samples between 1996 and 2002. The diagnosis of HastV was done by EIA and there were found 2,8% of global prevalence. Fourteen of the positive samples were typed by RT PCR and sequencing the 384bp region of the ORF2 segment of the viral genome (which codes for the capsid protein). Neighbor joining was done in order to make the phylogenetic study. The conclusions of the study were that in Colombia, HastV has an endemic behavior and the Colombian strains, obtained from samples of geographically distant or the same places, show that there is no genetic variability among the same genotype and among strains with the same typed but collected out of this country.

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“…At the present, the enteric adenoviruses of specie F, HAdV-40 and HAdV-41, are the third cause of non-bacterial diarrhea among young children (3,9). HAdV-41 is fatal in 50% of immunosupressed individuals infected (11).…”

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confidence: 99%

Longitudinal study on occurrence of adenoviruses and hepatitis A virus in raw domestic sewage in the city of Limeira, São Paulo

Barrella¹,

Garrafa²,

Monezi³

et al. 2009

Braz. J. Microbiol.

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The aim of this study was to verify the presence and annual distribution of adenoviruses and hepatitis A virus in domestic sewage in the city of Limeira, São Paulo. Fifty samples with a volume of 8 liters each were collected weekly from December 2004 to December 2005. The viruses were concentrated by filtration through positively charged ZP60S filter membranes, followed by ultracentrifugation. Human adenoviruses (HAdV) were detected by PCR followed by nested-PCR and screening for species F was done by restriction of the PCR product with TaqI endonuclease. Virus infectivity assays were performed by inoculation of concentrates onto HEp-2 cell monolayers. RT-PCR was used for the detection of hepatitis A virus. HAdV were detected in all samples, and 64% of samples were positive for infectious virus. Species F was present in 82% of the samples. Hepatitis A virus was detected in 48% of the samples. These results demonstrate that HAdV and HAV were present in the domestic sewage of Limeira throughout the period of study, demonstrating the importance of an adequate treatment before the disposal in the environment.

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Adenovirus genome types isolated from stools of children with gastroenteritis in São Paulo, Brazil

Cited by 31 publications

References 29 publications

Perfil etiológico das diarréias agudas de crianças atendidas em São Paulo

Perfil etiológico das diarréias agudas de crianças atendidas em São Paulo

Astrovirus (HAstV) como agente causal de diarrea en niños Colombianos: Siete años de estudio

Longitudinal study on occurrence of adenoviruses and hepatitis A virus in raw domestic sewage in the city of Limeira, São Paulo

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