Adenovirus-mediated gene transfer in olfactory neuronsin vivo

Zhao, Haiqing; Otaki, Joji M.; Firestein, Stuart

doi:10.1002/(sici)1097-4695(199608)30:4<521::aid-neu7>3.0.co;2-5

Cited by 39 publications

(19 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They used a strong OMP promoter to drive the expression in the nasal epithelium (Yoshihara et al 1999). They also showed that with adenoviral expression of WGA driven by the ubiquitous promoter CAG, transneuronal labeling was still observed, although already weaker (Zhao et al 1996). The AAV2/7 viral vector used in our study might not be the most suitable serotype for nasal transduction.…”

Section: Discussionmentioning

confidence: 77%

Evaluation of WGA–Cre-dependent topological transgene expression in the rodent brain

et al. 2016

View full text Add to dashboard Cite

Novel neuromodulation techniques in the field of brain research, such as optogenetics, prompt to target specific cell populations. However, not every subpopulation can be distinguished based on brain area or activity of specific promoters, but rather on topology and connectivity. A fascinating tool to detect neuronal circuitry is based on the transsynaptic tracer, wheat germ agglutinin (WGA). When expressed in neurons, it is transported throughout the neuron, secreted, and taken up by synaptically connected neurons. Expression of a WGA and Cre recombinase fusion protein using a viral vector technology in Cre-dependent transgenic animals allows to trace neuronal network connections and to induce topological transgene expression. In this study, we applied and evaluated this technology in specific areas throughout the whole rodent brain, including the hippocampus, striatum, substantia nigra, and the motor cortex. Adeno-associated viral vectors (rAAV) encoding the WGA-Cre fusion protein under control of a CMV promoter were stereotactically injected in Rosa26-STOP-EYFP transgenic mice. After 6 weeks, both the number of transneuronally labeled YFP ? / mCherry -cells and the transduced YFP ? /mCherry ? cells were quantified in the connected regions. We were able to trace several connections using WGA-Cre transneuronal labeling; however, the labeling efficacy was region-dependent. The observed transneuronal labeling mostly occurred in the anterograde direction without the occurrence of multi-synaptic labeling. Furthermore, we were able to visualize a specific subset of newborn neurons derived from the subventricular zone based on their connectivity.

show abstract

Section: Discussionmentioning

confidence: 77%

Evaluation of WGA–Cre-dependent topological transgene expression in the rodent brain

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Expression of the recombinant rat I7 odor receptor gene can be induced in rat olfactory epithelia with an engineered adenovirus vector 15 . Upon infection, I7 receptor expression is driven in many sensory neurons.…”

Section: Resultsmentioning

confidence: 99%

The molecular receptive range of an odorant receptor

2000

Self Cite

View full text Add to dashboard Cite

An odor perception is the brain's interpretation of the activation pattern of many peripheral sensory neurons that are differentially sensitive to a wide variety of odors. The sensitivity of these neurons is determined by which of the thousand or so odor receptor proteins they express on their surface. Understanding the odor code thus requires mapping the receptive range of odorant receptors. We have adopted a pharmacological approach that uses a large and diverse pool of odorous compounds to characterize the molecular receptive field of an odor receptor. We found a high specificity for certain molecular features, but high tolerance for others-a strategy that enables the olfactory apparatus to be both highly discriminating, and able to recognize several thousand odorous compounds.

show abstract

“…Intense staining was observed in the olfactory axons and their targets in the glomerular layer of the olfactory bulb with maximal expression at 3-10 days post-infection Zhao et al (1996) Expression levels were greater in the olfactory versus respiratory epithelia Aujeszky's disease (Pseudorabies)…”

Section: Metalsmentioning

confidence: 99%

Intranasal Administration of Neurotoxicants in Animals: Support for the Olfactory Vector Hypothesis of Parkinson’s Disease

et al. 2011

View full text Add to dashboard Cite

The causes of Parkinson's disease (PD) are unknown, but there is evidence that exposure to environmental agents, including a number of viruses, toxins, agricultural chemicals, dietary nutrients, and metals, is associated with its development in some cases. The presence of smell loss and the pathological involvement of the olfactory pathways in the early stages of PD are in accord with the tenants of the olfactory vector hypothesis. This hypothesis postulates that some forms of PD may be caused or catalyzed by environmental agents that enter the brain via the olfactory mucosa. In this article, we provide an overview of evidence implicating xenobiotics agents in the etiology of PD and review animal, mostly rodent, studies in which toxicants have been introduced into the nose in an attempt to induce behavioral or neurochemical changes similar to those seen in PD. The available data suggest that this route of exposure results in highly variable outcomes, depending upon the involved xenobiotic, exposure history, and the age and species of the animals tested. Some compounds, such as rotenone, paraquat, and 6-hydroxydopamine, have limited capacity to reach and damage the nigrostriatal dopaminergic system via the intranasal route. Others, such as 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), readily enter the brain via this route in some species and influence the function of the nigrostriatal pathway. Intranasal infusion of MPTP in some rodents elicits a developmental sequence of behavioral and neurochemical changes that closely mimics that seen in PD. For this reason, such an MPTP rodent model appears to be an ecologically valid means for assessing novel palliative treatments for both the motor and non-motor symptoms of PD. More research is needed, however, on this and other ecologically valid models.

show abstract

Adenovirus-mediated gene transfer in olfactory neuronsin vivo

Cited by 39 publications

References 18 publications

Evaluation of WGA–Cre-dependent topological transgene expression in the rodent brain

Evaluation of WGA–Cre-dependent topological transgene expression in the rodent brain

The molecular receptive range of an odorant receptor

Intranasal Administration of Neurotoxicants in Animals: Support for the Olfactory Vector Hypothesis of Parkinson’s Disease

Contact Info

Product

Resources

About